Isopod holobionts as promising models for lignocellulose degradation
Abstract Background Isopods have colonized all environments, partly thanks to their ability to decompose the organic matter. Their enzymatic repertoire, as well as the one of their associated microbiota, has contributed to their colonization success. Together, these holobionts have evolved several i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | http://link.springer.com/article/10.1186/s13068-020-01683-2 |
| _version_ | 1818554061587218432 |
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| author | Marius Bredon Benjamin Herran Joanne Bertaux Pierre Grève Bouziane Moumen Didier Bouchon |
| author_facet | Marius Bredon Benjamin Herran Joanne Bertaux Pierre Grève Bouziane Moumen Didier Bouchon |
| author_sort | Marius Bredon |
| collection | DOAJ |
| description | Abstract Background Isopods have colonized all environments, partly thanks to their ability to decompose the organic matter. Their enzymatic repertoire, as well as the one of their associated microbiota, has contributed to their colonization success. Together, these holobionts have evolved several interesting life history traits to degrade the plant cell walls, mainly composed of lignocellulose. It has been shown that terrestrial isopods achieve lignocellulose degradation thanks to numerous and diverse CAZymes provided by both the host and its microbiota. Nevertheless, the strategies for lignocellulose degradation seem more diversified in isopods, in particular in aquatic species which are the least studied. Isopods could be an interesting source of valuable enzymes for biotechnological industries of biomass conversion. Results To provide new features on the lignocellulose degradation in isopod holobionts, shotgun sequencing of 36 metagenomes of digestive and non-digestive tissues was performed from several populations of four aquatic and terrestrial isopod species. Combined to the 15 metagenomes of an additional species from our previous study, as well as the host transcriptomes, this large dataset allowed us to identify the CAZymes in both the host and the associated microbial communities. Analyses revealed the dominance of Bacteroidetes and Proteobacteria in the five species, covering 36% and 56% of the total bacterial community, respectively. The identification of CAZymes and new enzymatic systems for lignocellulose degradation, such as PULs, cellulosomes and LPMOs, highlights the richness of the strategies used by the isopods and their associated microbiota. Conclusions Altogether, our results show that the isopod holobionts are promising models to study lignocellulose degradation. These models can provide new enzymes and relevant lignocellulose-degrading bacteria strains for the biotechnological industries of biomass conversion. |
| first_indexed | 2024-12-12T09:34:13Z |
| format | Article |
| id | doaj.art-8c01cb38aba442d2b912ba03730825e7 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T09:34:13Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-8c01cb38aba442d2b912ba03730825e72022-12-22T00:28:46ZengBMCBiotechnology for Biofuels1754-68342020-03-0113111410.1186/s13068-020-01683-2Isopod holobionts as promising models for lignocellulose degradationMarius Bredon0Benjamin Herran1Joanne Bertaux2Pierre Grève3Bouziane Moumen4Didier Bouchon5Laboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de PoitiersLaboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de PoitiersLaboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de PoitiersLaboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de PoitiersLaboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de PoitiersLaboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de PoitiersAbstract Background Isopods have colonized all environments, partly thanks to their ability to decompose the organic matter. Their enzymatic repertoire, as well as the one of their associated microbiota, has contributed to their colonization success. Together, these holobionts have evolved several interesting life history traits to degrade the plant cell walls, mainly composed of lignocellulose. It has been shown that terrestrial isopods achieve lignocellulose degradation thanks to numerous and diverse CAZymes provided by both the host and its microbiota. Nevertheless, the strategies for lignocellulose degradation seem more diversified in isopods, in particular in aquatic species which are the least studied. Isopods could be an interesting source of valuable enzymes for biotechnological industries of biomass conversion. Results To provide new features on the lignocellulose degradation in isopod holobionts, shotgun sequencing of 36 metagenomes of digestive and non-digestive tissues was performed from several populations of four aquatic and terrestrial isopod species. Combined to the 15 metagenomes of an additional species from our previous study, as well as the host transcriptomes, this large dataset allowed us to identify the CAZymes in both the host and the associated microbial communities. Analyses revealed the dominance of Bacteroidetes and Proteobacteria in the five species, covering 36% and 56% of the total bacterial community, respectively. The identification of CAZymes and new enzymatic systems for lignocellulose degradation, such as PULs, cellulosomes and LPMOs, highlights the richness of the strategies used by the isopods and their associated microbiota. Conclusions Altogether, our results show that the isopod holobionts are promising models to study lignocellulose degradation. These models can provide new enzymes and relevant lignocellulose-degrading bacteria strains for the biotechnological industries of biomass conversion.http://link.springer.com/article/10.1186/s13068-020-01683-2LignocelluloseCAZymesShotgun metagenomicsMicrobiotaHolobiontTranscriptomics |
| spellingShingle | Marius Bredon Benjamin Herran Joanne Bertaux Pierre Grève Bouziane Moumen Didier Bouchon Isopod holobionts as promising models for lignocellulose degradation Biotechnology for Biofuels Lignocellulose CAZymes Shotgun metagenomics Microbiota Holobiont Transcriptomics |
| title | Isopod holobionts as promising models for lignocellulose degradation |
| title_full | Isopod holobionts as promising models for lignocellulose degradation |
| title_fullStr | Isopod holobionts as promising models for lignocellulose degradation |
| title_full_unstemmed | Isopod holobionts as promising models for lignocellulose degradation |
| title_short | Isopod holobionts as promising models for lignocellulose degradation |
| title_sort | isopod holobionts as promising models for lignocellulose degradation |
| topic | Lignocellulose CAZymes Shotgun metagenomics Microbiota Holobiont Transcriptomics |
| url | http://link.springer.com/article/10.1186/s13068-020-01683-2 |
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