Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia
Abstract Metabolomics may shed light on treatment response in childhood acute lymphoblastic leukemia (ALL), however, most assessments have analyzed bone marrow or cerebrospinal fluid (CSF), which are not collected during all phases of therapy. Blood is collected frequently and with fewer risks, but...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99147-6 |
| _version_ | 1818991062132195328 |
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| author | Jeremy M. Schraw J. P. Woodhouse Melanie B. Bernhardt Olga A. Taylor Terzah M. Horton Michael E. Scheurer M. Fatih Okcu Karen R. Rabin Philip J. Lupo Austin L. Brown |
| author_facet | Jeremy M. Schraw J. P. Woodhouse Melanie B. Bernhardt Olga A. Taylor Terzah M. Horton Michael E. Scheurer M. Fatih Okcu Karen R. Rabin Philip J. Lupo Austin L. Brown |
| author_sort | Jeremy M. Schraw |
| collection | DOAJ |
| description | Abstract Metabolomics may shed light on treatment response in childhood acute lymphoblastic leukemia (ALL), however, most assessments have analyzed bone marrow or cerebrospinal fluid (CSF), which are not collected during all phases of therapy. Blood is collected frequently and with fewer risks, but it is unclear whether findings from marrow or CSF biomarker studies may translate. We profiled end-induction plasma, marrow, and CSF from N = 10 children with B-ALL using liquid chromatography-mass spectrometry. We estimated correlations between plasma and marrow/CSF metabolite abundances detected in ≥ 3 patients using Spearman rank correlation coefficients (r s ). Most marrow metabolites were detected in plasma (N = 661; 81%), and we observed moderate-to-strong correlations (median r s 0.62, interquartile range [IQR] 0.29–0.83). We detected 328 CSF metabolites in plasma (90%); plasma-CSF correlations were weaker (median r s 0.37, IQR 0.07–0.70). We observed plasma-marrow correlations for metabolites in pathways associated with end-induction residual disease (pyruvate, asparagine) and plasma-CSF correlations for a biomarker of fatigue (gamma-glutamylglutamine). There is considerable overlap between the plasma, marrow, and CSF metabolomes, and we observed strong correlations for biomarkers of clinically relevant phenotypes. Plasma may be suitable for biomarker studies in B-ALL. |
| first_indexed | 2024-12-20T20:04:17Z |
| format | Article |
| id | doaj.art-8c04e83546a4413485aff2fad63ba637 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:04:17Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8c04e83546a4413485aff2fad63ba6372022-12-21T19:27:58ZengNature PortfolioScientific Reports2045-23222021-10-011111810.1038/s41598-021-99147-6Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemiaJeremy M. Schraw0J. P. Woodhouse1Melanie B. Bernhardt2Olga A. Taylor3Terzah M. Horton4Michael E. Scheurer5M. Fatih Okcu6Karen R. Rabin7Philip J. Lupo8Austin L. Brown9Section of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineSection of Hematology-Oncology, Department of Pediatrics, Baylor College of MedicineAbstract Metabolomics may shed light on treatment response in childhood acute lymphoblastic leukemia (ALL), however, most assessments have analyzed bone marrow or cerebrospinal fluid (CSF), which are not collected during all phases of therapy. Blood is collected frequently and with fewer risks, but it is unclear whether findings from marrow or CSF biomarker studies may translate. We profiled end-induction plasma, marrow, and CSF from N = 10 children with B-ALL using liquid chromatography-mass spectrometry. We estimated correlations between plasma and marrow/CSF metabolite abundances detected in ≥ 3 patients using Spearman rank correlation coefficients (r s ). Most marrow metabolites were detected in plasma (N = 661; 81%), and we observed moderate-to-strong correlations (median r s 0.62, interquartile range [IQR] 0.29–0.83). We detected 328 CSF metabolites in plasma (90%); plasma-CSF correlations were weaker (median r s 0.37, IQR 0.07–0.70). We observed plasma-marrow correlations for metabolites in pathways associated with end-induction residual disease (pyruvate, asparagine) and plasma-CSF correlations for a biomarker of fatigue (gamma-glutamylglutamine). There is considerable overlap between the plasma, marrow, and CSF metabolomes, and we observed strong correlations for biomarkers of clinically relevant phenotypes. Plasma may be suitable for biomarker studies in B-ALL.https://doi.org/10.1038/s41598-021-99147-6 |
| spellingShingle | Jeremy M. Schraw J. P. Woodhouse Melanie B. Bernhardt Olga A. Taylor Terzah M. Horton Michael E. Scheurer M. Fatih Okcu Karen R. Rabin Philip J. Lupo Austin L. Brown Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia Scientific Reports |
| title | Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia |
| title_full | Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia |
| title_fullStr | Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia |
| title_full_unstemmed | Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia |
| title_short | Comparison of the blood, bone marrow, and cerebrospinal fluid metabolomes in children with b-cell acute lymphoblastic leukemia |
| title_sort | comparison of the blood bone marrow and cerebrospinal fluid metabolomes in children with b cell acute lymphoblastic leukemia |
| url | https://doi.org/10.1038/s41598-021-99147-6 |
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