Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles
The use of metal oxide nanoparticles for the development of cost-effective glucose biosensors has been receiving increased attention. Enzymatic and non-enzymatic glucose sensor using polyethylene glycol (PEG) grafted manganese ferrite (PEG-MnFe _2 O _4 ) nanoparticles (NPs) modified onto a glassy ca...
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| Format: | Article |
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IOP Publishing
2020-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/abb32e |
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| author | Monunith A Arunima Rajan S Niroj Kumar Sahu |
| author_facet | Monunith A Arunima Rajan S Niroj Kumar Sahu |
| author_sort | Monunith A |
| collection | DOAJ |
| description | The use of metal oxide nanoparticles for the development of cost-effective glucose biosensors has been receiving increased attention. Enzymatic and non-enzymatic glucose sensor using polyethylene glycol (PEG) grafted manganese ferrite (PEG-MnFe _2 O _4 ) nanoparticles (NPs) modified onto a glassy carbon electrode (GCE) is reported in the present study. XRD and Raman studies confirmed the cubic spinel structure of MnFe _2 O _4. The immobilization of glucose oxidase (GOx) on PEG-MnFe _2 O _4 (GOx@PEG-MnFe _2 O _4 ) was validated using FTIR and TGA. Sensing electrodes exhibited well-defined redox peaks in 0.1 M phosphate buffered saline (PBS) solution at pH 7.4 against the reference electrode Ag/AgCl. GOx@PEG-MnFe _2 O _4 /GCE displayed a sensitivity of 1.985 μ A mM ^−1 cm ^−2 in the linear range of 1 to 20 mM with a limit of detection (LOD) of 0.132 mM whereas non-enzymatic sensor exhibited a sensitivity of 1.044 μ A mM ^−1 cm ^−2 in the linear range of 1 to 10 mM with a LOD of 0.099 mM. The lower Michaelis constant ( ${K}_{m}^{app}$ ) value indicates greater affinity towards glucose for the enzymatic sensor. GOx@PEG-MnFe _2 O _4 revealed selectivity specifically for glucose over various interferants such as fructose, lactic acid, sucrose, uric acid and ascorbic acid. In addition, this enzymatic sensor demonstrated better reproducibility and lifetime. |
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| language | English |
| last_indexed | 2024-03-12T15:34:33Z |
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| spelling | doaj.art-8c063dfc68cb4d4fb62dd46af890b54a2023-08-09T16:16:51ZengIOP PublishingMaterials Research Express2053-15912020-01-017909400110.1088/2053-1591/abb32eComparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticlesMonunith A0Arunima Rajan S1Niroj Kumar Sahu2https://orcid.org/0000-0002-0499-4108Centre for Nanotechnology Research, Vellore Institute of Technology, Vellore-632014, IndiaCentre for Nanotechnology Research, Vellore Institute of Technology, Vellore-632014, India; School of Advanced Sciences, Vellore Institute of Technology, Vellore-632014, IndiaCentre for Nanotechnology Research, Vellore Institute of Technology, Vellore-632014, IndiaThe use of metal oxide nanoparticles for the development of cost-effective glucose biosensors has been receiving increased attention. Enzymatic and non-enzymatic glucose sensor using polyethylene glycol (PEG) grafted manganese ferrite (PEG-MnFe _2 O _4 ) nanoparticles (NPs) modified onto a glassy carbon electrode (GCE) is reported in the present study. XRD and Raman studies confirmed the cubic spinel structure of MnFe _2 O _4. The immobilization of glucose oxidase (GOx) on PEG-MnFe _2 O _4 (GOx@PEG-MnFe _2 O _4 ) was validated using FTIR and TGA. Sensing electrodes exhibited well-defined redox peaks in 0.1 M phosphate buffered saline (PBS) solution at pH 7.4 against the reference electrode Ag/AgCl. GOx@PEG-MnFe _2 O _4 /GCE displayed a sensitivity of 1.985 μ A mM ^−1 cm ^−2 in the linear range of 1 to 20 mM with a limit of detection (LOD) of 0.132 mM whereas non-enzymatic sensor exhibited a sensitivity of 1.044 μ A mM ^−1 cm ^−2 in the linear range of 1 to 10 mM with a LOD of 0.099 mM. The lower Michaelis constant ( ${K}_{m}^{app}$ ) value indicates greater affinity towards glucose for the enzymatic sensor. GOx@PEG-MnFe _2 O _4 revealed selectivity specifically for glucose over various interferants such as fructose, lactic acid, sucrose, uric acid and ascorbic acid. In addition, this enzymatic sensor demonstrated better reproducibility and lifetime.https://doi.org/10.1088/2053-1591/abb32eglucose oxidasemanganese ferritenanoparticlesglucose sensingcyclic voltammetryamperometry |
| spellingShingle | Monunith A Arunima Rajan S Niroj Kumar Sahu Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles Materials Research Express glucose oxidase manganese ferrite nanoparticles glucose sensing cyclic voltammetry amperometry |
| title | Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles |
| title_full | Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles |
| title_fullStr | Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles |
| title_full_unstemmed | Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles |
| title_short | Comparative study of enzymatic and non-enzymatic detection of glucose using manganese ferrite nanoparticles |
| title_sort | comparative study of enzymatic and non enzymatic detection of glucose using manganese ferrite nanoparticles |
| topic | glucose oxidase manganese ferrite nanoparticles glucose sensing cyclic voltammetry amperometry |
| url | https://doi.org/10.1088/2053-1591/abb32e |
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