Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula
Abstract Background Plant architecture, which is mostly determined by shoot branching, plays an important role in plant growth and development. Thus, it is essential to explore the regulatory molecular mechanism of branching patterns based on the economic and ecological importance. In our previous w...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-11-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-2098-y |
| _version_ | 1819016687529230336 |
|---|---|
| author | Rui Han Chenrui Gu Ranhong Li Wendi Xu Shuo Wang Chaoyi Liu Chang Qu Su Chen Guifeng Liu Qibin Yu Jing Jiang Huiyu Li |
| author_facet | Rui Han Chenrui Gu Ranhong Li Wendi Xu Shuo Wang Chaoyi Liu Chang Qu Su Chen Guifeng Liu Qibin Yu Jing Jiang Huiyu Li |
| author_sort | Rui Han |
| collection | DOAJ |
| description | Abstract Background Plant architecture, which is mostly determined by shoot branching, plays an important role in plant growth and development. Thus, it is essential to explore the regulatory molecular mechanism of branching patterns based on the economic and ecological importance. In our previous work, a multiple-branches birch mutant br was identified from 19 CINNAMOYL-COENZYME A REDUCTASE 1 (CCR1)-overexpressed transgenic lines, and the expression patterns of differentially expressed genes in br were analyzed. In this study, we further explored some other characteristics of br, including plant architecture, wood properties, photosynthetic characteristics, and IAA and Zeatin contents. Meanwhile, the T-DNA insertion sites caused by the insertion of exogenous BpCCR1 in br were identified to explain the causes of the mutation phenotypes. Results The mutant br exhibited slower growth, more abundant and weaker branches, and lower wood basic density and lignin content than BpCCR1 transgenic line (OE2) and wild type (WT). Compared to WT and OE2, br had high stomatal conductance (Gs), transpiration rate (Tr), but a low non-photochemical quenching coefficient (NPQ) and chlorophyll content. In addition, br displayed an equal IAA and Zeatin content ratio of main branches’ apical buds to lateral branches’ apical buds and high ratio of Zeatin to IAA content. Two T-DNA insertion sites caused by the insertion of exogenous BpCCR1 in br genome were found. On one site, chromosome 2 (Chr2), no known gene was detected on the flanking sequence. The other site was on Chr5, with an insertion of 388 bp T-DNA sequence, resulting in deletion of 107 bp 5′ untranslated region (UTR) and 264 bp coding sequence (CDS) on CORONATINE INSENSITIVE 1 (BpCOII). In comparison with OE2 and WT, BpCOI1 was down-regulated in br, and the sensitivity of br to Methyl Jasmonate (MeJA) was abnormal. Conclusions Plant architecture, wood properties, photosynthetic characteristics, and IAA and Zeatin contents in main and lateral branches’ apical buds changed in br over the study’s time period. One T-DNA insertion was identified on the first exon of BpCOI1, which resulted in the reduction of BpCOI1 expression and abnormal perception to MeJA in br. These mutation phenotypes might be associated with a partial loss of BpCOI1 in birch. |
| first_indexed | 2024-12-21T02:51:35Z |
| format | Article |
| id | doaj.art-8c0649086c0e49bc80b8c6dc5488e402 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T02:51:35Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-8c0649086c0e49bc80b8c6dc5488e4022022-12-21T19:18:25ZengBMCBMC Plant Biology1471-22292019-11-0119111610.1186/s12870-019-2098-yCharacterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendulaRui Han0Chenrui Gu1Ranhong Li2Wendi Xu3Shuo Wang4Chaoyi Liu5Chang Qu6Su Chen7Guifeng Liu8Qibin Yu9Jing Jiang10Huiyu Li11State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityInstitute of Food and Agricultural Sciences, Citrus Research and Education Center, University of FloridaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityAbstract Background Plant architecture, which is mostly determined by shoot branching, plays an important role in plant growth and development. Thus, it is essential to explore the regulatory molecular mechanism of branching patterns based on the economic and ecological importance. In our previous work, a multiple-branches birch mutant br was identified from 19 CINNAMOYL-COENZYME A REDUCTASE 1 (CCR1)-overexpressed transgenic lines, and the expression patterns of differentially expressed genes in br were analyzed. In this study, we further explored some other characteristics of br, including plant architecture, wood properties, photosynthetic characteristics, and IAA and Zeatin contents. Meanwhile, the T-DNA insertion sites caused by the insertion of exogenous BpCCR1 in br were identified to explain the causes of the mutation phenotypes. Results The mutant br exhibited slower growth, more abundant and weaker branches, and lower wood basic density and lignin content than BpCCR1 transgenic line (OE2) and wild type (WT). Compared to WT and OE2, br had high stomatal conductance (Gs), transpiration rate (Tr), but a low non-photochemical quenching coefficient (NPQ) and chlorophyll content. In addition, br displayed an equal IAA and Zeatin content ratio of main branches’ apical buds to lateral branches’ apical buds and high ratio of Zeatin to IAA content. Two T-DNA insertion sites caused by the insertion of exogenous BpCCR1 in br genome were found. On one site, chromosome 2 (Chr2), no known gene was detected on the flanking sequence. The other site was on Chr5, with an insertion of 388 bp T-DNA sequence, resulting in deletion of 107 bp 5′ untranslated region (UTR) and 264 bp coding sequence (CDS) on CORONATINE INSENSITIVE 1 (BpCOII). In comparison with OE2 and WT, BpCOI1 was down-regulated in br, and the sensitivity of br to Methyl Jasmonate (MeJA) was abnormal. Conclusions Plant architecture, wood properties, photosynthetic characteristics, and IAA and Zeatin contents in main and lateral branches’ apical buds changed in br over the study’s time period. One T-DNA insertion was identified on the first exon of BpCOI1, which resulted in the reduction of BpCOI1 expression and abnormal perception to MeJA in br. These mutation phenotypes might be associated with a partial loss of BpCOI1 in birch.http://link.springer.com/article/10.1186/s12870-019-2098-yBetula platyphylla × Betula pendulaMultiple-branches mutantT-DNAInsertion and deletionBpCOI1 |
| spellingShingle | Rui Han Chenrui Gu Ranhong Li Wendi Xu Shuo Wang Chaoyi Liu Chang Qu Su Chen Guifeng Liu Qibin Yu Jing Jiang Huiyu Li Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula BMC Plant Biology Betula platyphylla × Betula pendula Multiple-branches mutant T-DNA Insertion and deletion BpCOI1 |
| title | Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula |
| title_full | Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula |
| title_fullStr | Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula |
| title_full_unstemmed | Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula |
| title_short | Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula |
| title_sort | characterization and t dna insertion sites identification of a multiple branches mutant br in betula platyphylla betula pendula |
| topic | Betula platyphylla × Betula pendula Multiple-branches mutant T-DNA Insertion and deletion BpCOI1 |
| url | http://link.springer.com/article/10.1186/s12870-019-2098-y |
| work_keys_str_mv | AT ruihan characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT chenruigu characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT ranhongli characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT wendixu characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT shuowang characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT chaoyiliu characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT changqu characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT suchen characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT guifengliu characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT qibinyu characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT jingjiang characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula AT huiyuli characterizationandtdnainsertionsitesidentificationofamultiplebranchesmutantbrinbetulaplatyphyllabetulapendula |