Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera)
Abstract Background Banana fruit scarring beetle (BFSB), Basilepta subcostata (Jac.) (Chrysomelidae:Coleoptera), is an important insect pest feeds on leaf and fingers, which affects the cosmetic value of the fruit. The pest is distributed in Assam, Bihar, West Bengal, Chhattisgarh, and North-eastern...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-03-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-021-00382-w |
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| author | Velavan Viswakethu Padmanaban Balakrishanan Loganathan Murugan Baskar Narayana swamy Uma Subbaraya |
| author_facet | Velavan Viswakethu Padmanaban Balakrishanan Loganathan Murugan Baskar Narayana swamy Uma Subbaraya |
| author_sort | Velavan Viswakethu |
| collection | DOAJ |
| description | Abstract Background Banana fruit scarring beetle (BFSB), Basilepta subcostata (Jac.) (Chrysomelidae:Coleoptera), is an important insect pest feeds on leaf and fingers, which affects the cosmetic value of the fruit. The pest is distributed in Assam, Bihar, West Bengal, Chhattisgarh, and North-eastern Hill regions of India. Results The pest is currently managed by foliar spray with insecticides. In order to identify eco-friendly control of the pest, attempts were made to isolate microbial agent and evaluate their potential to control the pest. A total of 27 entomopathogenic fugal isolates were obtained from Odoiporus longicollis (Oliver), Cosmopolites sordidus (Germar), Basilepta subcostata (Jac), and other insect Galleria mellonella (Fabr). Based on colony morphology, the collected fungal isolates were identified as Metarhizium spp. (17) and Beauveria spp. (10). Through ITS sequencing, the fungal isolates were further characterized at species level as B. bassiana (8), B. brongniartii (2), M. anisopliae (8), M. robertsii (6), M. guizhouense (2), and M. pinghaense (1). Their sequences were submitted in GenBank and obtained accession numbers. Among 27 isolates tested against B. subcostata under laboratory conditions, 3 isolates (M. anisopliae NRCBEPF-36, M. pinghaense NRCBEPF-7 and B. brongniartii NRCBEPF-27) recorded 100% beetle mortality, followed by 11 isolates with 95–99% and 13 isolates with 88–93% within 8 days of treatment. Conclusion This study highlights the two native North East India isolates B. brongniartii NRCBEPF-27 (MT151781) and M. anisopliae NRCBEPF-36 (MT140308) showed the significance to use as potential biocontrol agents against banana fruit scarring beetle B. subcostata. Further experiments under field conditions are required to evaluate their biological control efficacy against the pest. |
| first_indexed | 2024-12-17T07:53:30Z |
| format | Article |
| id | doaj.art-8c080ec84b9c4d22876b343c48767b27 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-17T07:53:30Z |
| publishDate | 2021-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-8c080ec84b9c4d22876b343c48767b272022-12-21T21:57:46ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422021-03-013111710.1186/s41938-021-00382-wEntomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera)Velavan Viswakethu0Padmanaban Balakrishanan1Loganathan Murugan2Baskar Narayana swamy3Uma Subbaraya4Division of Crop Protection, ICAR-National Research Center for BananaDivision of Crop Protection, ICAR-National Research Center for BananaDivision of Crop Protection, ICAR-National Research Center for BananaDivision of Crop Protection, ICAR-National Research Center for BananaDivision of Crop Protection, ICAR-National Research Center for BananaAbstract Background Banana fruit scarring beetle (BFSB), Basilepta subcostata (Jac.) (Chrysomelidae:Coleoptera), is an important insect pest feeds on leaf and fingers, which affects the cosmetic value of the fruit. The pest is distributed in Assam, Bihar, West Bengal, Chhattisgarh, and North-eastern Hill regions of India. Results The pest is currently managed by foliar spray with insecticides. In order to identify eco-friendly control of the pest, attempts were made to isolate microbial agent and evaluate their potential to control the pest. A total of 27 entomopathogenic fugal isolates were obtained from Odoiporus longicollis (Oliver), Cosmopolites sordidus (Germar), Basilepta subcostata (Jac), and other insect Galleria mellonella (Fabr). Based on colony morphology, the collected fungal isolates were identified as Metarhizium spp. (17) and Beauveria spp. (10). Through ITS sequencing, the fungal isolates were further characterized at species level as B. bassiana (8), B. brongniartii (2), M. anisopliae (8), M. robertsii (6), M. guizhouense (2), and M. pinghaense (1). Their sequences were submitted in GenBank and obtained accession numbers. Among 27 isolates tested against B. subcostata under laboratory conditions, 3 isolates (M. anisopliae NRCBEPF-36, M. pinghaense NRCBEPF-7 and B. brongniartii NRCBEPF-27) recorded 100% beetle mortality, followed by 11 isolates with 95–99% and 13 isolates with 88–93% within 8 days of treatment. Conclusion This study highlights the two native North East India isolates B. brongniartii NRCBEPF-27 (MT151781) and M. anisopliae NRCBEPF-36 (MT140308) showed the significance to use as potential biocontrol agents against banana fruit scarring beetle B. subcostata. Further experiments under field conditions are required to evaluate their biological control efficacy against the pest.https://doi.org/10.1186/s41938-021-00382-wBasilepta subcostataMetarhizium anisopliaeBeauveria bassianaITS regionBioassay |
| spellingShingle | Velavan Viswakethu Padmanaban Balakrishanan Loganathan Murugan Baskar Narayana swamy Uma Subbaraya Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera) Egyptian Journal of Biological Pest Control Basilepta subcostata Metarhizium anisopliae Beauveria bassiana ITS region Bioassay |
| title | Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera) |
| title_full | Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera) |
| title_fullStr | Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera) |
| title_full_unstemmed | Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera) |
| title_short | Entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle, Basilepta subcostata (Jac.) (Chrysomelidae: Coleoptera) |
| title_sort | entomopathogenic fungi as a promising biological control agent against banana fruit scarring beetle basilepta subcostata jac chrysomelidae coleoptera |
| topic | Basilepta subcostata Metarhizium anisopliae Beauveria bassiana ITS region Bioassay |
| url | https://doi.org/10.1186/s41938-021-00382-w |
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