<i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance
Heat shock proteins (HSPs) play important roles in various stress conditions. In this study, <i>CmHSP90.5</i>, whose expression is induced by heat and salt, was cloned from a chrysanthemum (<i>Chrysanthemum morifolium</i>) ‘<i>Jinba</i>’ and expressed in <i>...
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MDPI AG
2022-06-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/6/532 |
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| author | Xinhui Wang Jianpeng Wu Yue Wang Yuhan Jiang Fei Li Yu Chen Jiafu Jiang Likai Wang Zhiyong Guan Fadi Chen Sumei Chen |
| author_facet | Xinhui Wang Jianpeng Wu Yue Wang Yuhan Jiang Fei Li Yu Chen Jiafu Jiang Likai Wang Zhiyong Guan Fadi Chen Sumei Chen |
| author_sort | Xinhui Wang |
| collection | DOAJ |
| description | Heat shock proteins (HSPs) play important roles in various stress conditions. In this study, <i>CmHSP90.5</i>, whose expression is induced by heat and salt, was cloned from a chrysanthemum (<i>Chrysanthemum morifolium</i>) ‘<i>Jinba</i>’ and expressed in <i>Arabidopsis</i>. We found that CmHSP90.5 localized in the chloroplast. The heterologous expression of <i>CmHSP90.5</i> weakened the heat tolerance of <i>Arabidopsis</i> and reduced the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), indicating that CmHSP90.5-mediated heat stress sensitivity may be partially due to the regulation of active oxygen cleavage. The levels of expression of <i>AtHSP101</i>, <i>AtHSP15.7</i>, and <i>AtHSP17.6C</i> in <i>CmHSP90.5</i>-overexpressing plants decreased compared with those in wild-type (WT) plants under heat stress, indicating that these HSPs and CmHSP90.5 coregulate a plant’s heat stress tolerance. In addition, the salt stress tolerance of the <i>CmHSP90.5</i>-overexpressing <i>Arabidopsis</i> decreased compared with that of WT plants; <i>CmHSP90.5</i>-overexpressing plants showed increased Na<sup>+</sup> levels and decreased K<sup>+</sup> and proline levels compared with those of WT plants. Interestingly, the expression of stress-related genes, such as the Na<sup>+</sup>/H<sup>+</sup> antiporter encoding gene <i>SOS1</i>, high-affinity K+ transporter encoding gene <i>HKT1;1</i>, and proline synthesis gene <i>AtP5CS1</i>, decreased in <i>CmHSP90.5</i>-overexpressing plants under salt stress compared with those expressions in WT plants. Our findings lay a foundation for understanding the roles of <i>CmHSP90.5</i> in response to abiotic stresses in <i>chrysanthemum</i>. |
| first_indexed | 2024-03-09T23:39:05Z |
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| spelling | doaj.art-8c08207a3ff94f61bfe614eef1de4eac2023-11-23T16:55:44ZengMDPI AGHorticulturae2311-75242022-06-018653210.3390/horticulturae8060532<i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress ToleranceXinhui Wang0Jianpeng Wu1Yue Wang2Yuhan Jiang3Fei Li4Yu Chen5Jiafu Jiang6Likai Wang7Zhiyong Guan8Fadi Chen9Sumei Chen10State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaHeat shock proteins (HSPs) play important roles in various stress conditions. In this study, <i>CmHSP90.5</i>, whose expression is induced by heat and salt, was cloned from a chrysanthemum (<i>Chrysanthemum morifolium</i>) ‘<i>Jinba</i>’ and expressed in <i>Arabidopsis</i>. We found that CmHSP90.5 localized in the chloroplast. The heterologous expression of <i>CmHSP90.5</i> weakened the heat tolerance of <i>Arabidopsis</i> and reduced the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), indicating that CmHSP90.5-mediated heat stress sensitivity may be partially due to the regulation of active oxygen cleavage. The levels of expression of <i>AtHSP101</i>, <i>AtHSP15.7</i>, and <i>AtHSP17.6C</i> in <i>CmHSP90.5</i>-overexpressing plants decreased compared with those in wild-type (WT) plants under heat stress, indicating that these HSPs and CmHSP90.5 coregulate a plant’s heat stress tolerance. In addition, the salt stress tolerance of the <i>CmHSP90.5</i>-overexpressing <i>Arabidopsis</i> decreased compared with that of WT plants; <i>CmHSP90.5</i>-overexpressing plants showed increased Na<sup>+</sup> levels and decreased K<sup>+</sup> and proline levels compared with those of WT plants. Interestingly, the expression of stress-related genes, such as the Na<sup>+</sup>/H<sup>+</sup> antiporter encoding gene <i>SOS1</i>, high-affinity K+ transporter encoding gene <i>HKT1;1</i>, and proline synthesis gene <i>AtP5CS1</i>, decreased in <i>CmHSP90.5</i>-overexpressing plants under salt stress compared with those expressions in WT plants. Our findings lay a foundation for understanding the roles of <i>CmHSP90.5</i> in response to abiotic stresses in <i>chrysanthemum</i>.https://www.mdpi.com/2311-7524/8/6/532heat stresssalinityCmHSP90.5<i>chrysanthemum</i>transgenic <i>Arabidopsis</i> |
| spellingShingle | Xinhui Wang Jianpeng Wu Yue Wang Yuhan Jiang Fei Li Yu Chen Jiafu Jiang Likai Wang Zhiyong Guan Fadi Chen Sumei Chen <i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance Horticulturae heat stress salinity CmHSP90.5 <i>chrysanthemum</i> transgenic <i>Arabidopsis</i> |
| title | <i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance |
| title_full | <i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance |
| title_fullStr | <i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance |
| title_full_unstemmed | <i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance |
| title_short | <i>Chrysanthemum</i> <i>CmHSP90.5</i> as a Tool to Regulate Heat and Salt Stress Tolerance |
| title_sort | i chrysanthemum i i cmhsp90 5 i as a tool to regulate heat and salt stress tolerance |
| topic | heat stress salinity CmHSP90.5 <i>chrysanthemum</i> transgenic <i>Arabidopsis</i> |
| url | https://www.mdpi.com/2311-7524/8/6/532 |
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