Producing cadmium-free Indica rice by overexpressing OsHMA3
Background: Considerable proportions of rice grains produced in some areas in southern China contain high concentrations of cadmium (Cd), leading to unsafe levels of dietary Cd intake. Cultivars of Indica rice, widely grown in southern China, are particularly prone to high Cd accumulation in the gra...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-05-01
|
| Series: | Environment International |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412018332197 |
| _version_ | 1798045244581740544 |
|---|---|
| author | Chenni Lu Lingxiao Zhang Zhong Tang Xin-Yuan Huang Jian Feng Ma Fang-Jie Zhao |
| author_facet | Chenni Lu Lingxiao Zhang Zhong Tang Xin-Yuan Huang Jian Feng Ma Fang-Jie Zhao |
| author_sort | Chenni Lu |
| collection | DOAJ |
| description | Background: Considerable proportions of rice grains produced in some areas in southern China contain high concentrations of cadmium (Cd), leading to unsafe levels of dietary Cd intake. Cultivars of Indica rice, widely grown in southern China, are particularly prone to high Cd accumulation in the grain. Effective methods are needed to decrease Cd accumulation in Indica rice. Methods: OsHMA3, encoding a tonoplast Cd transporter, was overexpressed in an elite Indica rice cultivar (Zhongjiazao 17) driven by CaMV 35S promoter. The effects on Cd translocation, accumulation and tolerance, as well as on the agronomic traits and micronutrient concentrations were evaluated. Results: OsHMA3 overexpression markedly decreased Cd translocation from roots to shoots and increased Cd tolerance. OsHMA3 overexpression decreased Cd concentrations in brown rice by 94–98%, to levels just above the detection limit, when rice plants were grown in two Cd-contaminated paddy soils. OsHMA3 overexpression generally had no significant effect on grain yield and the concentrations of the essential micronutrients including zinc, iron, copper and manganese in field trials. Conclusion: Overexpression of OsHMA3 is a highly effective method to reduce Cd accumulation in Indica rice, producing rice grains that were almost Cd free with little effect on grain yield or essential micronutrient concentrations. Keywords: Cadmium, Food safety, OsHMA3, Genetic modification, Rice |
| first_indexed | 2024-04-11T23:19:05Z |
| format | Article |
| id | doaj.art-8c108c1828b847608bc33c7bd5c31bfb |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-04-11T23:19:05Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-8c108c1828b847608bc33c7bd5c31bfb2022-12-22T03:57:32ZengElsevierEnvironment International0160-41202019-05-01126619626Producing cadmium-free Indica rice by overexpressing OsHMA3Chenni Lu0Lingxiao Zhang1Zhong Tang2Xin-Yuan Huang3Jian Feng Ma4Fang-Jie Zhao5State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, JapanState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author.Background: Considerable proportions of rice grains produced in some areas in southern China contain high concentrations of cadmium (Cd), leading to unsafe levels of dietary Cd intake. Cultivars of Indica rice, widely grown in southern China, are particularly prone to high Cd accumulation in the grain. Effective methods are needed to decrease Cd accumulation in Indica rice. Methods: OsHMA3, encoding a tonoplast Cd transporter, was overexpressed in an elite Indica rice cultivar (Zhongjiazao 17) driven by CaMV 35S promoter. The effects on Cd translocation, accumulation and tolerance, as well as on the agronomic traits and micronutrient concentrations were evaluated. Results: OsHMA3 overexpression markedly decreased Cd translocation from roots to shoots and increased Cd tolerance. OsHMA3 overexpression decreased Cd concentrations in brown rice by 94–98%, to levels just above the detection limit, when rice plants were grown in two Cd-contaminated paddy soils. OsHMA3 overexpression generally had no significant effect on grain yield and the concentrations of the essential micronutrients including zinc, iron, copper and manganese in field trials. Conclusion: Overexpression of OsHMA3 is a highly effective method to reduce Cd accumulation in Indica rice, producing rice grains that were almost Cd free with little effect on grain yield or essential micronutrient concentrations. Keywords: Cadmium, Food safety, OsHMA3, Genetic modification, Ricehttp://www.sciencedirect.com/science/article/pii/S0160412018332197 |
| spellingShingle | Chenni Lu Lingxiao Zhang Zhong Tang Xin-Yuan Huang Jian Feng Ma Fang-Jie Zhao Producing cadmium-free Indica rice by overexpressing OsHMA3 Environment International |
| title | Producing cadmium-free Indica rice by overexpressing OsHMA3 |
| title_full | Producing cadmium-free Indica rice by overexpressing OsHMA3 |
| title_fullStr | Producing cadmium-free Indica rice by overexpressing OsHMA3 |
| title_full_unstemmed | Producing cadmium-free Indica rice by overexpressing OsHMA3 |
| title_short | Producing cadmium-free Indica rice by overexpressing OsHMA3 |
| title_sort | producing cadmium free indica rice by overexpressing oshma3 |
| url | http://www.sciencedirect.com/science/article/pii/S0160412018332197 |
| work_keys_str_mv | AT chennilu producingcadmiumfreeindicaricebyoverexpressingoshma3 AT lingxiaozhang producingcadmiumfreeindicaricebyoverexpressingoshma3 AT zhongtang producingcadmiumfreeindicaricebyoverexpressingoshma3 AT xinyuanhuang producingcadmiumfreeindicaricebyoverexpressingoshma3 AT jianfengma producingcadmiumfreeindicaricebyoverexpressingoshma3 AT fangjiezhao producingcadmiumfreeindicaricebyoverexpressingoshma3 |