Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
Although several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/7/234 |
| _version_ | 1797406984564113408 |
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| author | Pearl Thompson Ana Lívia de Carvalho Bovolato Gisela Ibáñez-Redín Alexandre G. Brolo |
| author_facet | Pearl Thompson Ana Lívia de Carvalho Bovolato Gisela Ibáñez-Redín Alexandre G. Brolo |
| author_sort | Pearl Thompson |
| collection | DOAJ |
| description | Although several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This assay provided the sensitive detection of IgG antibodies against SARS-CoV-2 within 10 min. We started by synthesising, modifying, and characterising gold nanoparticles. Using these particles as a coloured label, we developed a lateral flow strip made of nitrocellulose, glass fibre, and cellulose material. We quantified our visual results using pictures acquired with a cell phone and calculated a limit of detection of 4 ng/mL of antibodies against the SARS-CoV-2 spike protein. |
| first_indexed | 2024-03-09T03:34:42Z |
| format | Article |
| id | doaj.art-8c113ff953394833b580fbccba24b470 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T03:34:42Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-8c113ff953394833b580fbccba24b4702023-12-03T14:50:11ZengMDPI AGChemosensors2227-90402022-06-0110723410.3390/chemosensors10070234Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell PhonePearl Thompson0Ana Lívia de Carvalho Bovolato1Gisela Ibáñez-Redín2Alexandre G. Brolo3Department of Chemistry, University of Victoria, Victoria, BC V8P 5C2, CanadaDepartment of Chemistry, University of Victoria, Victoria, BC V8P 5C2, CanadaDepartment of Chemistry, University of Victoria, Victoria, BC V8P 5C2, CanadaDepartment of Chemistry, University of Victoria, Victoria, BC V8P 5C2, CanadaAlthough several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This assay provided the sensitive detection of IgG antibodies against SARS-CoV-2 within 10 min. We started by synthesising, modifying, and characterising gold nanoparticles. Using these particles as a coloured label, we developed a lateral flow strip made of nitrocellulose, glass fibre, and cellulose material. We quantified our visual results using pictures acquired with a cell phone and calculated a limit of detection of 4 ng/mL of antibodies against the SARS-CoV-2 spike protein.https://www.mdpi.com/2227-9040/10/7/234lateral flowsalivaCOVID-19IgGSARS-CoV-2gold nanoparticles |
| spellingShingle | Pearl Thompson Ana Lívia de Carvalho Bovolato Gisela Ibáñez-Redín Alexandre G. Brolo Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone Chemosensors lateral flow saliva COVID-19 IgG SARS-CoV-2 gold nanoparticles |
| title | Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone |
| title_full | Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone |
| title_fullStr | Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone |
| title_full_unstemmed | Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone |
| title_short | Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone |
| title_sort | quantification of a covid 19 antibody assay using a lateral flow test and a cell phone |
| topic | lateral flow saliva COVID-19 IgG SARS-CoV-2 gold nanoparticles |
| url | https://www.mdpi.com/2227-9040/10/7/234 |
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