Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats
Background: Panax ginseng has a wide range of biological activities including anti-inflammatory, antioxidant, and immunomodulatory functions. Wild ginseng cambial meristematic cells (CMCs) were obtained from P. ginseng cambium. This study examined the protective mechanism of wild ginseng CMCs agains...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Journal of Ginseng Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315000354 |
| _version_ | 1818206228478689280 |
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| author | Seok-Joo Kim Hyo-Sun Choi Hong-Ik Cho Young-Woo Jin Eun-Kyong Lee Jeung Youb Ahn Sun-Mee Lee |
| author_facet | Seok-Joo Kim Hyo-Sun Choi Hong-Ik Cho Young-Woo Jin Eun-Kyong Lee Jeung Youb Ahn Sun-Mee Lee |
| author_sort | Seok-Joo Kim |
| collection | DOAJ |
| description | Background: Panax ginseng has a wide range of biological activities including anti-inflammatory, antioxidant, and immunomodulatory functions. Wild ginseng cambial meristematic cells (CMCs) were obtained from P. ginseng cambium. This study examined the protective mechanism of wild ginseng CMCs against d-galactosamine (GalN)-induced liver injury. GalN, a well-known hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals.
Methods: Hepatotoxicity was induced in rats using GalN (700 mg/kg, i.p.). Wild ginseng CMCs was administered orally once a day for 2 wks, and then 2 h prior to and 6 h after GalN injection.
Results: Wild ginseng CMCs attenuated the increase in serum aminotransferase activity that occurs 24 h after GalN injection. Wild ginseng CMCs also attenuated the GalN-induced increase in serum tumor necrosis factor-α, interleukin-6 level, and hepatic cyclooxygenase-2 protein and mRNA expression. Wild ginseng CMCs augmented the increase in serum interleukin -10 and hepatic heme oxygenase-1 protein and mRNA expression that was induced by GalN, inhibited the increase in the nuclear level of nuclear factor-kappa B, and enhanced the increase in NF-E2-related factor 2.
Conclusion: Our findings suggest that wild ginseng CMCs protects liver against GalN-induced inflammation by suppressing proinflammatory mediators and enhancing production of anti-inflammatory mediators. |
| first_indexed | 2024-12-12T04:09:42Z |
| format | Article |
| id | doaj.art-8c118d7450514723ae69f7242a8a83cd |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-12T04:09:42Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-8c118d7450514723ae69f7242a8a83cd2022-12-22T00:38:40ZengElsevierJournal of Ginseng Research1226-84532015-10-0139437638310.1016/j.jgr.2015.04.002Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in ratsSeok-Joo Kim0Hyo-Sun Choi1Hong-Ik Cho2Young-Woo Jin3Eun-Kyong Lee4Jeung Youb Ahn5Sun-Mee Lee6School of Pharmacy, Sungkyunkwan University, Suwon, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon, KoreaPlant Stem Cell Institute, Unhwa Corp., Jeonju, KoreaPlant Stem Cell Institute, Unhwa Corp., Jeonju, KoreaPlant Stem Cell Institute, Unhwa Corp., Jeonju, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon, KoreaBackground: Panax ginseng has a wide range of biological activities including anti-inflammatory, antioxidant, and immunomodulatory functions. Wild ginseng cambial meristematic cells (CMCs) were obtained from P. ginseng cambium. This study examined the protective mechanism of wild ginseng CMCs against d-galactosamine (GalN)-induced liver injury. GalN, a well-known hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals. Methods: Hepatotoxicity was induced in rats using GalN (700 mg/kg, i.p.). Wild ginseng CMCs was administered orally once a day for 2 wks, and then 2 h prior to and 6 h after GalN injection. Results: Wild ginseng CMCs attenuated the increase in serum aminotransferase activity that occurs 24 h after GalN injection. Wild ginseng CMCs also attenuated the GalN-induced increase in serum tumor necrosis factor-α, interleukin-6 level, and hepatic cyclooxygenase-2 protein and mRNA expression. Wild ginseng CMCs augmented the increase in serum interleukin -10 and hepatic heme oxygenase-1 protein and mRNA expression that was induced by GalN, inhibited the increase in the nuclear level of nuclear factor-kappa B, and enhanced the increase in NF-E2-related factor 2. Conclusion: Our findings suggest that wild ginseng CMCs protects liver against GalN-induced inflammation by suppressing proinflammatory mediators and enhancing production of anti-inflammatory mediators.http://www.sciencedirect.com/science/article/pii/S1226845315000354d-galactosamineheme oxygenase-1hepatitisPanax ginsengwild ginseng cambial meristematic cells |
| spellingShingle | Seok-Joo Kim Hyo-Sun Choi Hong-Ik Cho Young-Woo Jin Eun-Kyong Lee Jeung Youb Ahn Sun-Mee Lee Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats Journal of Ginseng Research d-galactosamine heme oxygenase-1 hepatitis Panax ginseng wild ginseng cambial meristematic cells |
| title | Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats |
| title_full | Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats |
| title_fullStr | Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats |
| title_full_unstemmed | Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats |
| title_short | Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats |
| title_sort | protective effect of wild ginseng cambial meristematic cells on d galactosamine induced hepatotoxicity in rats |
| topic | d-galactosamine heme oxygenase-1 hepatitis Panax ginseng wild ginseng cambial meristematic cells |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315000354 |
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