DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY
Objective of research: Development of methods for the determination of fenbendazole and its metabolites in milk by liquid chromatography coupled with tandem mass spectrometry.Materials and methods: Fenbendazole was administered orally to five cows. Samples of milk were taken on 1, 3, 5 and 10 days o...
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| Format: | Article |
| Language: | Russian |
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Federal Scientific Centre VIEV
2016-12-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/316 |
| _version_ | 1826570060910559232 |
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| author | P. P. Kochetkov A. I. Varlamova V. E. Abramov N. S. Misura E. V. Abramova S. B. Abramov N. I. Koshevarov I. A. Arkhipov |
| author_facet | P. P. Kochetkov A. I. Varlamova V. E. Abramov N. S. Misura E. V. Abramova S. B. Abramov N. I. Koshevarov I. A. Arkhipov |
| author_sort | P. P. Kochetkov |
| collection | DOAJ |
| description | Objective of research: Development of methods for the determination of fenbendazole and its metabolites in milk by liquid chromatography coupled with tandem mass spectrometry.Materials and methods: Fenbendazole was administered orally to five cows. Samples of milk were taken on 1, 3, 5 and 10 days of drug application. The research method includes a description of reagents, plates and equipment; mass-spectrometric conditions for analysis of fenbendazole and its metabolites; preparation of the equipment to operation; preparation of eluent solution; preparation of the chromatograph to analysis; determination of chromatographic parameters of standard drug samples; preparation of milk samples to analysis; establishment of parameters of extracts’ chromatography; procedure of calibration of fenbendazole and its metabolites in eluent.Results and discussion: When studying the pharmacokinetics of fenbendazole and its metabolites (sulfone and sulfoxide) in milk, it was found that the maximal concentrations were determined 24 h after drug administration and were 22,6 ng/ml for fenbendazole sulfone, 34,0 ng/ml for fenbendazole sulfoxide and 19,7 ng/ml for fenbendazole. 10 days after treatment, the concentrations of fenbendazole and its metabolites in milk did not exceed permitted values. |
| first_indexed | 2024-03-08T21:41:17Z |
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| id | doaj.art-8c133599f8e840a682aff67725f2d070 |
| institution | Directory Open Access Journal |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| last_indexed | 2025-03-14T11:49:14Z |
| publishDate | 2016-12-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj.art-8c133599f8e840a682aff67725f2d0702025-03-02T09:49:56ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432016-12-0138455456210.12737/23082316DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRYP. P. Kochetkov0A. I. Varlamova1V. E. Abramov2N. S. Misura3E. V. Abramova4S. B. Abramov5N. I. Koshevarov6I. A. Arkhipov7All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin; International Research Center for protection of human health, animals and environment.All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. SkryabinAll-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. SkryabinInternational Research Center for protection of human health, animals and environmentAll-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin; International Research Center for protection of human health, animals and environmentAll-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin; International Research Center for protection of human health, animals and environmentInternational Research Center for protection of human health, animals and environmentAll-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. SkryabinObjective of research: Development of methods for the determination of fenbendazole and its metabolites in milk by liquid chromatography coupled with tandem mass spectrometry.Materials and methods: Fenbendazole was administered orally to five cows. Samples of milk were taken on 1, 3, 5 and 10 days of drug application. The research method includes a description of reagents, plates and equipment; mass-spectrometric conditions for analysis of fenbendazole and its metabolites; preparation of the equipment to operation; preparation of eluent solution; preparation of the chromatograph to analysis; determination of chromatographic parameters of standard drug samples; preparation of milk samples to analysis; establishment of parameters of extracts’ chromatography; procedure of calibration of fenbendazole and its metabolites in eluent.Results and discussion: When studying the pharmacokinetics of fenbendazole and its metabolites (sulfone and sulfoxide) in milk, it was found that the maximal concentrations were determined 24 h after drug administration and were 22,6 ng/ml for fenbendazole sulfone, 34,0 ng/ml for fenbendazole sulfoxide and 19,7 ng/ml for fenbendazole. 10 days after treatment, the concentrations of fenbendazole and its metabolites in milk did not exceed permitted values.https://vniigis.elpub.ru/jour/article/view/316milkfenbendazolemetabolitesulfoxidesulfonepharmacokineticsliquid chromatographymass-spectrometry |
| spellingShingle | P. P. Kochetkov A. I. Varlamova V. E. Abramov N. S. Misura E. V. Abramova S. B. Abramov N. I. Koshevarov I. A. Arkhipov DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY Российский паразитологический журнал milk fenbendazole metabolite sulfoxide sulfone pharmacokinetics liquid chromatography mass-spectrometry |
| title | DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY |
| title_full | DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY |
| title_fullStr | DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY |
| title_full_unstemmed | DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY |
| title_short | DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY |
| title_sort | determination of fenbendazole and its metabolites in milk by the method of liquid chromatography coupled with tandem mass spectrometry |
| topic | milk fenbendazole metabolite sulfoxide sulfone pharmacokinetics liquid chromatography mass-spectrometry |
| url | https://vniigis.elpub.ru/jour/article/view/316 |
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