Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes
Context Anemarsaponin BII is one of the most active saponins isolated from Anemarrhena asphodeloides Bunge (Asparagaceae), a commonly used Chinese traditional paediatric medicine. Objective This study investigates the effects of anemarsaponin BII on the activity of CYP450s to provide more guidance f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2020.1835996 |
| _version_ | 1818832950487154688 |
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| author | Mingwei Wang Wei Jiang Juan Zhou Xiujuan Xue Changlong Yin |
| author_facet | Mingwei Wang Wei Jiang Juan Zhou Xiujuan Xue Changlong Yin |
| author_sort | Mingwei Wang |
| collection | DOAJ |
| description | Context Anemarsaponin BII is one of the most active saponins isolated from Anemarrhena asphodeloides Bunge (Asparagaceae), a commonly used Chinese traditional paediatric medicine. Objective This study investigates the effects of anemarsaponin BII on the activity of CYP450s to provide more guidance for the clinical use of anemarsaponin BII. Materials and methods Using various diagnostic substrates, the effects of a fixed concentration of anemarsaponin BII (100 μM) on the activity of eight main isoforms of CYP450s (CYP1A2, 2A6, 3A4, 2C8, 2C9, 2C19, 2D6 and 2E1) was first studied with pooled human liver microsomes (HLMs). Then, dose-dependent (0, 2.5, 5, 10, 25, 50 and 100 μM anemarsaponin BII) and time-dependent (0, 5, 10, 15 and 30 min) experiments were performed to obtain corresponding kinetic parameters. Results Anemarsaponin BII showed significant inhibitory effects on the activity of CYP3A4, 2D6 and 2E1 with the IC50 values of 13.67, 16.26 and 19.72 μM. Anemarsaponin BII acted as a non-competitive inhibitor of CYP3A4 with the KI value of 6.72 μM and competitive inhibitors of CYP2D6 and 2E1 with the KI values of 8.26 and 9.82 μM, respectively. Additionally, the inhibition of CYP3A4 was revealed to be time-dependent with the KI value of 4.88 μM and the Kinact value of 0.053/min. Conclusions The inhibitory effect of anemarsaponin BII on the activity of CYP3A4, 2D6 and 2E1 indicated the potential drug–drug interaction between anemarsaponin BII and drugs metabolized by these CYP450s. Further in vivo experiments are needed to validate the potential drug–drug interactions. |
| first_indexed | 2024-12-19T02:11:10Z |
| format | Article |
| id | doaj.art-8c14937705524d4d94e0d8d7abaf8f76 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-19T02:11:10Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-8c14937705524d4d94e0d8d7abaf8f762022-12-21T20:40:44ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-015811064106910.1080/13880209.2020.18359961835996Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomesMingwei Wang0Wei Jiang1Juan Zhou2Xiujuan Xue3Changlong Yin4Department of Pediatrics, Yidu Central Hospital of WeifangDepartment of Pediatrics, Yidu Central Hospital of WeifangDepartment of Pediatrics, Yidu Central Hospital of WeifangDepartment of Pediatrics, Yidu Central Hospital of WeifangDepartment of Pediatrics, Yidu Central Hospital of WeifangContext Anemarsaponin BII is one of the most active saponins isolated from Anemarrhena asphodeloides Bunge (Asparagaceae), a commonly used Chinese traditional paediatric medicine. Objective This study investigates the effects of anemarsaponin BII on the activity of CYP450s to provide more guidance for the clinical use of anemarsaponin BII. Materials and methods Using various diagnostic substrates, the effects of a fixed concentration of anemarsaponin BII (100 μM) on the activity of eight main isoforms of CYP450s (CYP1A2, 2A6, 3A4, 2C8, 2C9, 2C19, 2D6 and 2E1) was first studied with pooled human liver microsomes (HLMs). Then, dose-dependent (0, 2.5, 5, 10, 25, 50 and 100 μM anemarsaponin BII) and time-dependent (0, 5, 10, 15 and 30 min) experiments were performed to obtain corresponding kinetic parameters. Results Anemarsaponin BII showed significant inhibitory effects on the activity of CYP3A4, 2D6 and 2E1 with the IC50 values of 13.67, 16.26 and 19.72 μM. Anemarsaponin BII acted as a non-competitive inhibitor of CYP3A4 with the KI value of 6.72 μM and competitive inhibitors of CYP2D6 and 2E1 with the KI values of 8.26 and 9.82 μM, respectively. Additionally, the inhibition of CYP3A4 was revealed to be time-dependent with the KI value of 4.88 μM and the Kinact value of 0.053/min. Conclusions The inhibitory effect of anemarsaponin BII on the activity of CYP3A4, 2D6 and 2E1 indicated the potential drug–drug interaction between anemarsaponin BII and drugs metabolized by these CYP450s. Further in vivo experiments are needed to validate the potential drug–drug interactions.http://dx.doi.org/10.1080/13880209.2020.1835996cyp450 enzymescyp2d6cyp2e1drug–drug interaction |
| spellingShingle | Mingwei Wang Wei Jiang Juan Zhou Xiujuan Xue Changlong Yin Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes Pharmaceutical Biology cyp450 enzymes cyp2d6 cyp2e1 drug–drug interaction |
| title | Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes |
| title_full | Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes |
| title_fullStr | Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes |
| title_full_unstemmed | Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes |
| title_short | Anemarsaponin BII inhibits the activity of CYP3A4, 2D6, and 2E1 with human liver microsomes |
| title_sort | anemarsaponin bii inhibits the activity of cyp3a4 2d6 and 2e1 with human liver microsomes |
| topic | cyp450 enzymes cyp2d6 cyp2e1 drug–drug interaction |
| url | http://dx.doi.org/10.1080/13880209.2020.1835996 |
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