Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]
In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies. However, in eukaryotes, the functional role of HGTs remains questionable, although current genomic tools are providing i...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2015-12-01
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| Online Access: | http://f1000research.com/articles/4-1523/v1 |
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| author | Punita Sharma Tanwee Das De Swati Sharma Ashwani Kumar Mishra Tina Thomas Sonia Verma Vandana Kumari Suman Lata Namita Singh Neena Valecha Kailash Chand Pandey Rajnikant Dixit |
| author_facet | Punita Sharma Tanwee Das De Swati Sharma Ashwani Kumar Mishra Tina Thomas Sonia Verma Vandana Kumari Suman Lata Namita Singh Neena Valecha Kailash Chand Pandey Rajnikant Dixit |
| author_sort | Punita Sharma |
| collection | DOAJ |
| description | In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies. However, in eukaryotes, the functional role of HGTs remains questionable, although current genomic tools are providing increased evidence of acquisition of novel traits within non-mating metazoan species. Here, we provide another transcriptomic evidence for the acquisition of massive plant genes in the mosquito, Anopheles culicifacies. Our multiple experimental validations including genomic PCR, RT-PCR, real-time PCR, immuno-blotting and immuno-florescence microscopy, confirmed that plant like transcripts (PLTs) are of mosquito origin and may encode functional proteins. A comprehensive molecular analysis of the PLTs and ongoing metagenomic analysis of salivary microbiome provide initial clues that mosquitoes may have survival benefits through the acquisition of nuclear as well as chloroplast encoded plant genes. Our findings of PLTs further support the similar questionable observation of HGTs in other higher organisms, which is still a controversial and debatable issue in the community of evolutionists. We believe future understanding of the underlying mechanism of the feeding associated molecular responses may shed new insights in the functional role of PLTs in the mosquito. |
| first_indexed | 2024-04-13T18:57:19Z |
| format | Article |
| id | doaj.art-8c197b2608e945978332582ef52f7729 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-04-13T18:57:19Z |
| publishDate | 2015-12-01 |
| publisher | F1000 Research Ltd |
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| series | F1000Research |
| spelling | doaj.art-8c197b2608e945978332582ef52f77292022-12-22T02:34:11ZengF1000 Research LtdF1000Research2046-14022015-12-01410.12688/f1000research.7534.18113Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]Punita Sharma0Tanwee Das De1Swati Sharma2Ashwani Kumar Mishra3Tina Thomas4Sonia Verma5Vandana Kumari6Suman Lata7Namita Singh8Neena Valecha9Kailash Chand Pandey10Rajnikant Dixit11Nano and Biotechnology Department, Guru Jambheshwar University, Haryana, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaNxGenBio Lifesciences, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaNano and Biotechnology Department, Guru Jambheshwar University, Haryana, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaHost-Parasite Interaction Biology Group, National Institute of Malaria Research, Delhi, IndiaIn prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies. However, in eukaryotes, the functional role of HGTs remains questionable, although current genomic tools are providing increased evidence of acquisition of novel traits within non-mating metazoan species. Here, we provide another transcriptomic evidence for the acquisition of massive plant genes in the mosquito, Anopheles culicifacies. Our multiple experimental validations including genomic PCR, RT-PCR, real-time PCR, immuno-blotting and immuno-florescence microscopy, confirmed that plant like transcripts (PLTs) are of mosquito origin and may encode functional proteins. A comprehensive molecular analysis of the PLTs and ongoing metagenomic analysis of salivary microbiome provide initial clues that mosquitoes may have survival benefits through the acquisition of nuclear as well as chloroplast encoded plant genes. Our findings of PLTs further support the similar questionable observation of HGTs in other higher organisms, which is still a controversial and debatable issue in the community of evolutionists. We believe future understanding of the underlying mechanism of the feeding associated molecular responses may shed new insights in the functional role of PLTs in the mosquito.http://f1000research.com/articles/4-1523/v1Directed Molecular EvolutionGenomics |
| spellingShingle | Punita Sharma Tanwee Das De Swati Sharma Ashwani Kumar Mishra Tina Thomas Sonia Verma Vandana Kumari Suman Lata Namita Singh Neena Valecha Kailash Chand Pandey Rajnikant Dixit Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved] F1000Research Directed Molecular Evolution Genomics |
| title | Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved] |
| title_full | Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved] |
| title_fullStr | Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved] |
| title_full_unstemmed | Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved] |
| title_short | Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved] |
| title_sort | deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito anopheles culicifacies an evolutionary puzzle version 1 referees 2 approved |
| topic | Directed Molecular Evolution Genomics |
| url | http://f1000research.com/articles/4-1523/v1 |
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