| Summary: | <p>Abstract</p> <p>Background</p> <p>Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis.</p> <p>Results</p> <p>For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for <it>Portucel </it>and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10<sup>-13 </sup>mol/mm<sup>2 </sup>was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers.</p> <p>Conclusion</p> <p>It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).</p>
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