Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice
Objective Pulegone (PGN) is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the (R)-(+) PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptorα (ERα) and ERβ receptors e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2018-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/journal/article/19689/download |
| _version_ | 1818193800280932352 |
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| author | Rohiyeh Souldouzi Mazdak Razi Ali Shalizar Jalali Ghader Jalilzadeh-Amin Saeedeh Amani |
| author_facet | Rohiyeh Souldouzi Mazdak Razi Ali Shalizar Jalali Ghader Jalilzadeh-Amin Saeedeh Amani |
| author_sort | Rohiyeh Souldouzi |
| collection | DOAJ |
| description | Objective
Pulegone (PGN) is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the (R)-(+) PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptorα (ERα) and ERβ receptors expressions.
Materials and Methods
In this experimental study, mature albino mice were divided into experimental (received 25 mg/kg, 50 mg/kg and 100 mg/kg PGN, orally for 35 days) and control (received 2% solution of Tween 80 as a PGN solvent, orally) groups. The mRNA levels of Erα, Erβ, p53, Bcl-2, and cytochrome p450 (Cyp19) as well as ovarian angiogenesis were analyzed through reverse transcription polymerase chain reaction and immunohistochemical techniques, respectively. Moreover, apoptosis of follicular cells, serum estrogen and progesterone levels and mRNA damage were investigated via using terminal transferase and biotin-16-dUTP staining, electrochemilunescence and fluorescent microscopy methods, respectively.
Results
The PGN reduced Erα, Erβ and Cyp19 expression at 50 mg/kg and 100 mg/kg doses, while significantly elevating p53 and reducing Bcl-2 expression. Finally, PGN impaired ovarian angiogenesis, increased apoptosis, elevated follicular atresia and reduced serum levels of estrogen and progesterone.
Conclusion
Chronic exposure to PGN (50 mg/kg and 100 mg/kg), severely affects ovarian aromatization, proto- oncogenes mRNA levels and expression of ERs. |
| first_indexed | 2024-12-12T00:52:09Z |
| format | Article |
| id | doaj.art-8c1b358ee7074fca8044e4d0193d4668 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-12T00:52:09Z |
| publishDate | 2018-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-8c1b358ee7074fca8044e4d0193d46682022-12-22T00:43:59ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142018-04-0120223124310.22074/cellj.2018.4798Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in MiceRohiyeh Souldouzi0Mazdak Razi1Ali Shalizar Jalali2Ghader Jalilzadeh-Amin3Saeedeh Amani4Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranObjective Pulegone (PGN) is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the (R)-(+) PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptorα (ERα) and ERβ receptors expressions. Materials and Methods In this experimental study, mature albino mice were divided into experimental (received 25 mg/kg, 50 mg/kg and 100 mg/kg PGN, orally for 35 days) and control (received 2% solution of Tween 80 as a PGN solvent, orally) groups. The mRNA levels of Erα, Erβ, p53, Bcl-2, and cytochrome p450 (Cyp19) as well as ovarian angiogenesis were analyzed through reverse transcription polymerase chain reaction and immunohistochemical techniques, respectively. Moreover, apoptosis of follicular cells, serum estrogen and progesterone levels and mRNA damage were investigated via using terminal transferase and biotin-16-dUTP staining, electrochemilunescence and fluorescent microscopy methods, respectively. Results The PGN reduced Erα, Erβ and Cyp19 expression at 50 mg/kg and 100 mg/kg doses, while significantly elevating p53 and reducing Bcl-2 expression. Finally, PGN impaired ovarian angiogenesis, increased apoptosis, elevated follicular atresia and reduced serum levels of estrogen and progesterone. Conclusion Chronic exposure to PGN (50 mg/kg and 100 mg/kg), severely affects ovarian aromatization, proto- oncogenes mRNA levels and expression of ERs.http://celljournal.org/journal/article/19689/downloadApoptosisAromatizationMiceOvaryPulegone |
| spellingShingle | Rohiyeh Souldouzi Mazdak Razi Ali Shalizar Jalali Ghader Jalilzadeh-Amin Saeedeh Amani Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice Cell Journal Apoptosis Aromatization Mice Ovary Pulegone |
| title | Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice |
| title_full | Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice |
| title_fullStr | Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice |
| title_full_unstemmed | Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice |
| title_short | Effect of (R)-(+) Pulegone on Ovarian Tissue; Correlation with Expression of Aromatase Cyp19 and Ovarian Selected Genes in Mice |
| title_sort | effect of r pulegone on ovarian tissue correlation with expression of aromatase cyp19 and ovarian selected genes in mice |
| topic | Apoptosis Aromatization Mice Ovary Pulegone |
| url | http://celljournal.org/journal/article/19689/download |
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