Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia
Background and Aim: Subclinical mastitis (SCM) in Etawah-grade (PE) goats in Yogyakarta, Indonesia, is commonly due to Staphylococcus aureus. At present, S. aureus from SCM in PE goats in Yogyakarta has not been characterized. Therefore, this study aimed to phenotypically characterize S. aureus, whi...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-11-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/November-2022/10.pdf |
| _version_ | 1811314022068256768 |
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| author | Widodo Suwito Widagdo Sri Nugroho Rahmat Setya Adji Andriani Andriani Eny Kusumaningtyas Tri Martini |
| author_facet | Widodo Suwito Widagdo Sri Nugroho Rahmat Setya Adji Andriani Andriani Eny Kusumaningtyas Tri Martini |
| author_sort | Widodo Suwito |
| collection | DOAJ |
| description | Background and Aim: Subclinical mastitis (SCM) in Etawah-grade (PE) goats in Yogyakarta, Indonesia, is commonly due to Staphylococcus aureus. At present, S. aureus from SCM in PE goats in Yogyakarta has not been characterized. Therefore, this study aimed to phenotypically characterize S. aureus, which has been isolated from SCM of PE goats.
Materials and Methods: A total of 314 lactating PE goats were collected from 60 PE goat farms (e.g., Sleman, Bantul, and Kulonprogo) located in parts of Yogyakarta with an average age of 3–4 years old, three of which showed SCM based on the California mastitis test (CMT). Subclinical mastitis is confirmed in PE goats if CMT shows ++ or +++. Furthermore, S. aureus was detected by biochemical assays. Staphylococcus aureus could determine hemolysin (Hae), coagulase (Coa), clumping factor (Cf), and antibiotic susceptibility. Hemolytic bacteria were detected by culturing on blood agar plate, and Cf was detected by slide agglutination. The production of Coa was detected by tube coagulation. Staphylococcus aureus susceptibility was determined by antimicrobial agar diffusion using a paper disc.
Results: Phenotypically characterized S. aureus from PE goats with SCM in Yogyakarta, Indonesia, Coa–, Cf–, and Hae– were found to be resistant to erythromycin (ERYTHRO), ampicillin (AMP), penicillin (PEN-G), and sulfamethoxazole (SULFA).
Conclusion: The phenotypic characteristic of S. aureus, which was obtained from SCM in PE goats in Yogyakarta, consists of Coa, and Cf–. S. aureus cannot perform hemolysis of red blood cells. This phenotypic characteristic can prevent and control SCM in PE goats. Several antibiotics such as ERYTHRO, AMP, PEN-G, and SULFA were no longer effective for treating SCM in PE goats because S. aureus has developed its resistance to these antibiotics. |
| first_indexed | 2024-04-13T11:04:39Z |
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| id | doaj.art-8c1d5ea43ac2442f967c76792eec877b |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-13T11:04:39Z |
| publishDate | 2022-11-01 |
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| series | Veterinary World |
| spelling | doaj.art-8c1d5ea43ac2442f967c76792eec877b2022-12-22T02:49:19ZengVeterinary WorldVeterinary World0972-89882231-09162022-11-0115112587259210.14202/vetworld.2022.2587-2592Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, IndonesiaWidodo Suwito0https://orcid.org/0000-0002-1439-5862Widagdo Sri Nugroho1https://orcid.org/0000-0002-4984-6731Rahmat Setya Adji2https://orcid.org/0000-0002-9950-7260Andriani Andriani3https://orcid.org/0000-0002-0624-1580Eny Kusumaningtyas4https://orcid.org/0000-0002-9711-1035Tri Martini5https://orcid.org/0000-0002-2316-6033Research Center Processing Food Technology, National Research Innovation Agency (BRIN), Gunungkidul 55861, Yogyakarta, Indonesia.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Sleman 55281, Yogyakarta, Indonesia.Veterinary Research Center, National Research Innovation Agency (BRIN), Bogor 16124, West Java, Indonesia.Veterinary Research Center, National Research Innovation Agency (BRIN), Bogor 16124, West Java, Indonesia.Veterinary Research Center, National Research Innovation Agency (BRIN), Bogor 16124, West Java, Indonesia.Research Center Sustainable Production System and Life Cycle Assessment, Serpong 15311, Banten, Indonesia.Background and Aim: Subclinical mastitis (SCM) in Etawah-grade (PE) goats in Yogyakarta, Indonesia, is commonly due to Staphylococcus aureus. At present, S. aureus from SCM in PE goats in Yogyakarta has not been characterized. Therefore, this study aimed to phenotypically characterize S. aureus, which has been isolated from SCM of PE goats. Materials and Methods: A total of 314 lactating PE goats were collected from 60 PE goat farms (e.g., Sleman, Bantul, and Kulonprogo) located in parts of Yogyakarta with an average age of 3–4 years old, three of which showed SCM based on the California mastitis test (CMT). Subclinical mastitis is confirmed in PE goats if CMT shows ++ or +++. Furthermore, S. aureus was detected by biochemical assays. Staphylococcus aureus could determine hemolysin (Hae), coagulase (Coa), clumping factor (Cf), and antibiotic susceptibility. Hemolytic bacteria were detected by culturing on blood agar plate, and Cf was detected by slide agglutination. The production of Coa was detected by tube coagulation. Staphylococcus aureus susceptibility was determined by antimicrobial agar diffusion using a paper disc. Results: Phenotypically characterized S. aureus from PE goats with SCM in Yogyakarta, Indonesia, Coa–, Cf–, and Hae– were found to be resistant to erythromycin (ERYTHRO), ampicillin (AMP), penicillin (PEN-G), and sulfamethoxazole (SULFA). Conclusion: The phenotypic characteristic of S. aureus, which was obtained from SCM in PE goats in Yogyakarta, consists of Coa, and Cf–. S. aureus cannot perform hemolysis of red blood cells. This phenotypic characteristic can prevent and control SCM in PE goats. Several antibiotics such as ERYTHRO, AMP, PEN-G, and SULFA were no longer effective for treating SCM in PE goats because S. aureus has developed its resistance to these antibiotics.http://www.veterinaryworld.org/Vol.15/November-2022/10.pdfetawah-grade goatsphenotypicstaphylococcus aureussubclinical mastitis |
| spellingShingle | Widodo Suwito Widagdo Sri Nugroho Rahmat Setya Adji Andriani Andriani Eny Kusumaningtyas Tri Martini Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia Veterinary World etawah-grade goats phenotypic staphylococcus aureus subclinical mastitis |
| title | Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia |
| title_full | Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia |
| title_fullStr | Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia |
| title_full_unstemmed | Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia |
| title_short | Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia |
| title_sort | phenotypic characteristic of staphylococcus aureus from subclinical mastitis in etawah crossbreed goats in yogyakarta indonesia |
| topic | etawah-grade goats phenotypic staphylococcus aureus subclinical mastitis |
| url | http://www.veterinaryworld.org/Vol.15/November-2022/10.pdf |
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