Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity
The silver nanoparticles (AgNPs) synthesized using hot water olive leaf extracts (OLE) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against drug resistant bacterial isolates. The effect of extract concentration, contact time, pH and temperature on the react...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-12-01
|
| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535213000968 |
| _version_ | 1819207372882575360 |
|---|---|
| author | Mostafa M.H. Khalil Eman H. Ismail Khaled Z. El-Baghdady Doaa Mohamed |
| author_facet | Mostafa M.H. Khalil Eman H. Ismail Khaled Z. El-Baghdady Doaa Mohamed |
| author_sort | Mostafa M.H. Khalil |
| collection | DOAJ |
| description | The silver nanoparticles (AgNPs) synthesized using hot water olive leaf extracts (OLE) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against drug resistant bacterial isolates. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles are investigated. The data revealed that the rate of formation of the nanosilver increased significantly in the basic medium with increasing temperature. The nature of AgNPs synthesized was analyzed by UV–vis spectroscopy, X-ray diffraction, scanning electron microscopy and thermal gravimetric analysis (TGA). The silver nanoparticles were with an average size of 20–25 nm and mostly spherical. The antibacterial potential of synthesized AgNPs was compared with that of aqueous OLE by well diffusion method. The AgNPs at 0.03–0.07 mg/ml concentration significantly inhibited bacterial growth against multi drug resistant Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). This study revealed that the aqueous olive leaf extract has no effect at the concentrations used for preparation of the Ag nanoparticles. Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future. |
| first_indexed | 2024-12-23T05:22:27Z |
| format | Article |
| id | doaj.art-8c20b8d5a7114bc98bc35b9a661e449a |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-23T05:22:27Z |
| publishDate | 2014-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-8c20b8d5a7114bc98bc35b9a661e449a2022-12-21T17:58:40ZengElsevierArabian Journal of Chemistry1878-53522014-12-01761131113910.1016/j.arabjc.2013.04.007Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activityMostafa M.H. Khalil0Eman H. Ismail1Khaled Z. El-Baghdady2Doaa Mohamed3Chemistry Department, Faculty of Science, Ain Shams University, 11566 Abbassia, Cairo, EgyptChemistry Department, Faculty of Science, Ain Shams University, 11566 Abbassia, Cairo, EgyptMicrobiology Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, EgyptChemistry Department, Faculty of Science, Ain Shams University, 11566 Abbassia, Cairo, EgyptThe silver nanoparticles (AgNPs) synthesized using hot water olive leaf extracts (OLE) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against drug resistant bacterial isolates. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles are investigated. The data revealed that the rate of formation of the nanosilver increased significantly in the basic medium with increasing temperature. The nature of AgNPs synthesized was analyzed by UV–vis spectroscopy, X-ray diffraction, scanning electron microscopy and thermal gravimetric analysis (TGA). The silver nanoparticles were with an average size of 20–25 nm and mostly spherical. The antibacterial potential of synthesized AgNPs was compared with that of aqueous OLE by well diffusion method. The AgNPs at 0.03–0.07 mg/ml concentration significantly inhibited bacterial growth against multi drug resistant Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). This study revealed that the aqueous olive leaf extract has no effect at the concentrations used for preparation of the Ag nanoparticles. Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future.http://www.sciencedirect.com/science/article/pii/S1878535213000968NanosilverOlive leaf extractAntibacterial activityGreen synthesis |
| spellingShingle | Mostafa M.H. Khalil Eman H. Ismail Khaled Z. El-Baghdady Doaa Mohamed Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity Arabian Journal of Chemistry Nanosilver Olive leaf extract Antibacterial activity Green synthesis |
| title | Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity |
| title_full | Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity |
| title_fullStr | Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity |
| title_full_unstemmed | Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity |
| title_short | Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity |
| title_sort | green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity |
| topic | Nanosilver Olive leaf extract Antibacterial activity Green synthesis |
| url | http://www.sciencedirect.com/science/article/pii/S1878535213000968 |
| work_keys_str_mv | AT mostafamhkhalil greensynthesisofsilvernanoparticlesusingoliveleafextractanditsantibacterialactivity AT emanhismail greensynthesisofsilvernanoparticlesusingoliveleafextractanditsantibacterialactivity AT khaledzelbaghdady greensynthesisofsilvernanoparticlesusingoliveleafextractanditsantibacterialactivity AT doaamohamed greensynthesisofsilvernanoparticlesusingoliveleafextractanditsantibacterialactivity |