Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice
The gut microbiota is important in regulating host metabolism, maintaining physiology, and protecting immune homeostasis. Gut microbiota dysbiosis affects the development of the gut microenvironment, as well as the onset of various external systemic diseases and metabolic syndromes. Cyclophosphamide...
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MDPI AG
2022-03-01
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| author | Asif Iqbal Khan Ata Ur Rehman Nabeel Ahmed Farooqui Nimra Zafar Siddiqui Qamar Ayub Muhammad Noman Ramzan Liang Wang Yi Xin |
| author_facet | Asif Iqbal Khan Ata Ur Rehman Nabeel Ahmed Farooqui Nimra Zafar Siddiqui Qamar Ayub Muhammad Noman Ramzan Liang Wang Yi Xin |
| author_sort | Asif Iqbal Khan |
| collection | DOAJ |
| description | The gut microbiota is important in regulating host metabolism, maintaining physiology, and protecting immune homeostasis. Gut microbiota dysbiosis affects the development of the gut microenvironment, as well as the onset of various external systemic diseases and metabolic syndromes. Cyclophosphamide (CTX) is a commonly used chemotherapeutic drug that suppresses the host immune system, intestinal mucosa inflammation, and dysbiosis of the intestinal flora. Immunomodulators are necessary to enhance the immune system and prevent homeostasis disbalance and cytotoxicity caused by CTX. In this study, shrimp peptide hydrolysate (SPH) was evaluated for immunomodulation, intestinal integration, and microbiota in CTX-induced immunosuppressed mice. It was observed that SPH would significantly restore goblet cells and intestinal mucosa integrity, modulate the immune system, and increase relative expression of mRNA and tight-junction associated proteins (Occludin, Zo-1, Claudin-1, and Mucin-2). It also improved gut flora and restored the intestinal microbiota ecological balance by removing harmful microbes of various taxonomic groups. This would also increase the immune organs index, serum levels of cytokines (IFN-ϒ, IL1β, TNF-α, IL-6), and immunoglobin levels (IgA, IgM). The Firmicutes/Bacteroidetes proportion was decreased in CTX-induced mice. Finally, SPH would be recommended as a functional food source with a modulatory effect not only on intestinal microbiota, but also as a potential health-promoting immune function regulator. |
| first_indexed | 2024-03-09T20:29:17Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:29:17Z |
| publishDate | 2022-03-01 |
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| series | Molecules |
| spelling | doaj.art-8c21257ec43a4ef08543b5f87a63c7d12023-11-23T23:28:45ZengMDPI AGMolecules1420-30492022-03-01275172010.3390/molecules27051720Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated MiceAsif Iqbal Khan0Ata Ur Rehman1Nabeel Ahmed Farooqui2Nimra Zafar Siddiqui3Qamar Ayub4Muhammad Noman Ramzan5Liang Wang6Yi Xin7Department of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaCollege of Clinical Laboratory Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaStem Cell Clinical Research Center, National Joint Engineering Laboratory, Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, No. 193, Lianhe Road, Shahekou District, Dalian 116011, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaThe gut microbiota is important in regulating host metabolism, maintaining physiology, and protecting immune homeostasis. Gut microbiota dysbiosis affects the development of the gut microenvironment, as well as the onset of various external systemic diseases and metabolic syndromes. Cyclophosphamide (CTX) is a commonly used chemotherapeutic drug that suppresses the host immune system, intestinal mucosa inflammation, and dysbiosis of the intestinal flora. Immunomodulators are necessary to enhance the immune system and prevent homeostasis disbalance and cytotoxicity caused by CTX. In this study, shrimp peptide hydrolysate (SPH) was evaluated for immunomodulation, intestinal integration, and microbiota in CTX-induced immunosuppressed mice. It was observed that SPH would significantly restore goblet cells and intestinal mucosa integrity, modulate the immune system, and increase relative expression of mRNA and tight-junction associated proteins (Occludin, Zo-1, Claudin-1, and Mucin-2). It also improved gut flora and restored the intestinal microbiota ecological balance by removing harmful microbes of various taxonomic groups. This would also increase the immune organs index, serum levels of cytokines (IFN-ϒ, IL1β, TNF-α, IL-6), and immunoglobin levels (IgA, IgM). The Firmicutes/Bacteroidetes proportion was decreased in CTX-induced mice. Finally, SPH would be recommended as a functional food source with a modulatory effect not only on intestinal microbiota, but also as a potential health-promoting immune function regulator.https://www.mdpi.com/1420-3049/27/5/1720shrimp (<i>Penaeus Chinensis</i>)peptidesimmunosuppression gut microbiotacyclophosphamidegut health |
| spellingShingle | Asif Iqbal Khan Ata Ur Rehman Nabeel Ahmed Farooqui Nimra Zafar Siddiqui Qamar Ayub Muhammad Noman Ramzan Liang Wang Yi Xin Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice Molecules shrimp (<i>Penaeus Chinensis</i>) peptides immunosuppression gut microbiota cyclophosphamide gut health |
| title | Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice |
| title_full | Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice |
| title_fullStr | Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice |
| title_full_unstemmed | Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice |
| title_short | Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice |
| title_sort | effects of shrimp peptide hydrolysate on intestinal microbiota restoration and immune modulation in cyclophosphamide treated mice |
| topic | shrimp (<i>Penaeus Chinensis</i>) peptides immunosuppression gut microbiota cyclophosphamide gut health |
| url | https://www.mdpi.com/1420-3049/27/5/1720 |
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