Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions
Nicotiana benthamiana is the most widely used experimental host in plant virology, due mainly to the large number of diverse plant viruses that can successfully infect it. Additionally, N. benthamiana is susceptible to a wide variety of other plant-pathogenic agents (such as bacteria, oomycetes, fun...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-8-1015 |
| _version_ | 1818757347808378880 |
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| author | Michael M. Goodin David Zaitlin Rayapati A. Naidu Steven A. Lommel |
| author_facet | Michael M. Goodin David Zaitlin Rayapati A. Naidu Steven A. Lommel |
| author_sort | Michael M. Goodin |
| collection | DOAJ |
| description | Nicotiana benthamiana is the most widely used experimental host in plant virology, due mainly to the large number of diverse plant viruses that can successfully infect it. Additionally, N. benthamiana is susceptible to a wide variety of other plant-pathogenic agents (such as bacteria, oomycetes, fungi, and so on), making this species a cornerstone of host–pathogen research, particularly in the context of innate immunity and defense signaling. Moreover, because it can be genetically transformed and regenerated with good efficiency and is amenable to facile methods for virus-induced gene silencing or transient protein expression, N. benthamiana is rapidly gaining popularity in plant biology, particularly in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. Paradoxically, despite being an indispensable research model, little is known about the origins, genetic variation, or ecology of the N. benthamiana accessions currently used by the research community. In addition to addressing these latter topics, the purpose of this review is to provide information regarding sources for tools and reagents that can be used to support research in N. benthamiana. Finally, we propose that N. benthamiana is well situated to become a premier plant cell biology model, particularly for the virology community, who as a group were the first to recognize the potential of this unique Australian native. |
| first_indexed | 2024-12-18T06:09:30Z |
| format | Article |
| id | doaj.art-8c2230cb8ef040e786924db5b4bbcff9 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:30Z |
| publishDate | 2008-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-8c2230cb8ef040e786924db5b4bbcff92022-12-21T21:18:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-08-012181015102610.1094/MPMI-21-8-1015Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen InteractionsMichael M. GoodinDavid ZaitlinRayapati A. NaiduSteven A. LommelNicotiana benthamiana is the most widely used experimental host in plant virology, due mainly to the large number of diverse plant viruses that can successfully infect it. Additionally, N. benthamiana is susceptible to a wide variety of other plant-pathogenic agents (such as bacteria, oomycetes, fungi, and so on), making this species a cornerstone of host–pathogen research, particularly in the context of innate immunity and defense signaling. Moreover, because it can be genetically transformed and regenerated with good efficiency and is amenable to facile methods for virus-induced gene silencing or transient protein expression, N. benthamiana is rapidly gaining popularity in plant biology, particularly in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. Paradoxically, despite being an indispensable research model, little is known about the origins, genetic variation, or ecology of the N. benthamiana accessions currently used by the research community. In addition to addressing these latter topics, the purpose of this review is to provide information regarding sources for tools and reagents that can be used to support research in N. benthamiana. Finally, we propose that N. benthamiana is well situated to become a premier plant cell biology model, particularly for the virology community, who as a group were the first to recognize the potential of this unique Australian native.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-8-1015AFLPagroinfiltrationArabidopsisVIGS |
| spellingShingle | Michael M. Goodin David Zaitlin Rayapati A. Naidu Steven A. Lommel Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions Molecular Plant-Microbe Interactions AFLP agroinfiltration Arabidopsis VIGS |
| title | Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions |
| title_full | Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions |
| title_fullStr | Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions |
| title_full_unstemmed | Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions |
| title_short | Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions |
| title_sort | nicotiana benthamiana its history and future as a model for plant pathogen interactions |
| topic | AFLP agroinfiltration Arabidopsis VIGS |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-8-1015 |
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