Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration
MicroRNAs refer to a class of endogenous, short non-coding RNAs that mediate numerous biological functions. MicroRNAs regulate various physiological and pathological activities of peripheral nerves, including peripheral nerve repair and regeneration. Previously, using a rat sciatic nerve injury mode...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=9;spage=1651;epage=1656;aulast=Ji |
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| author | Xi-Meng Ji Shan-Shan Wang Xiao-Dong Cai Xing-Hui Wang Qian-Yan Liu Pan Wang Zhang-Chun Cheng Tian-Mei Qian |
| author_facet | Xi-Meng Ji Shan-Shan Wang Xiao-Dong Cai Xing-Hui Wang Qian-Yan Liu Pan Wang Zhang-Chun Cheng Tian-Mei Qian |
| author_sort | Xi-Meng Ji |
| collection | DOAJ |
| description | MicroRNAs refer to a class of endogenous, short non-coding RNAs that mediate numerous biological functions. MicroRNAs regulate various physiological and pathological activities of peripheral nerves, including peripheral nerve repair and regeneration. Previously, using a rat sciatic nerve injury model, we identified many functionally annotated novel microRNAs, including miR-sc14. Here, we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps. Our results show that miR-sc14 is noticeably altered following sciatic nerve injury, being up-regulated at 1 day and diminished at 7 days. EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells, while miR-sc14 inhibitor suppressed their proliferation and migration. Additionally, bioinformatic analysis examined potential target genes of miR-sc14, and found that fibroblast growth factor receptor 2 might be a potential target gene. Specifically, our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury. Appropriately, up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells. Consequently, miR-sc14 may be an intervention target to promote repair of peripheral nerve injury. The study was approved by the Jiangsu Provincial Laboratory Animal Management Committee, China on March 4, 2015 (approval No. 20150304-004). |
| first_indexed | 2024-12-22T05:23:41Z |
| format | Article |
| id | doaj.art-8c223ff13c49441291ce0ff2f4f75675 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-22T05:23:41Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-8c223ff13c49441291ce0ff2f4f756752022-12-21T18:37:39ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742019-01-011491651165610.4103/1673-5374.255996Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migrationXi-Meng JiShan-Shan WangXiao-Dong CaiXing-Hui WangQian-Yan LiuPan WangZhang-Chun ChengTian-Mei QianMicroRNAs refer to a class of endogenous, short non-coding RNAs that mediate numerous biological functions. MicroRNAs regulate various physiological and pathological activities of peripheral nerves, including peripheral nerve repair and regeneration. Previously, using a rat sciatic nerve injury model, we identified many functionally annotated novel microRNAs, including miR-sc14. Here, we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps. Our results show that miR-sc14 is noticeably altered following sciatic nerve injury, being up-regulated at 1 day and diminished at 7 days. EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells, while miR-sc14 inhibitor suppressed their proliferation and migration. Additionally, bioinformatic analysis examined potential target genes of miR-sc14, and found that fibroblast growth factor receptor 2 might be a potential target gene. Specifically, our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury. Appropriately, up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells. Consequently, miR-sc14 may be an intervention target to promote repair of peripheral nerve injury. The study was approved by the Jiangsu Provincial Laboratory Animal Management Committee, China on March 4, 2015 (approval No. 20150304-004).http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=9;spage=1651;epage=1656;aulast=Jinerve regeneration; novel microRNAs; miR-sc14; peripheral nerve injury; cell proliferation; cell migration; Schwann cells; fibroblast growth factor receptor 2; biological functions; peripheral nerve regeneration; regulatory mechanisms; neural regeneration |
| spellingShingle | Xi-Meng Ji Shan-Shan Wang Xiao-Dong Cai Xing-Hui Wang Qian-Yan Liu Pan Wang Zhang-Chun Cheng Tian-Mei Qian Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration Neural Regeneration Research nerve regeneration; novel microRNAs; miR-sc14; peripheral nerve injury; cell proliferation; cell migration; Schwann cells; fibroblast growth factor receptor 2; biological functions; peripheral nerve regeneration; regulatory mechanisms; neural regeneration |
| title | Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration |
| title_full | Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration |
| title_fullStr | Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration |
| title_full_unstemmed | Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration |
| title_short | Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration |
| title_sort | novel mirna mir sc14 promotes schwann cell proliferation and migration |
| topic | nerve regeneration; novel microRNAs; miR-sc14; peripheral nerve injury; cell proliferation; cell migration; Schwann cells; fibroblast growth factor receptor 2; biological functions; peripheral nerve regeneration; regulatory mechanisms; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=9;spage=1651;epage=1656;aulast=Ji |
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