Identification of Estrus in Sows Based on Salivary Proteomics
The estrus cycle of multiparous Large White sows was divided into three stages to solve the problems of heavy workload and low accuracy of the traditional estrus identification method in pig production. Saliva protein was extracted from the oral saliva of multiparous sows. Label-free quantitative pr...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/13/1656 |
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| author | Chenlei Li Chenglei Song Kunlong Qi Yingke Liu Yaqing Dou Xiuling Li Ruimin Qiao Kejun Wang Xuelei Han Xinjian Li |
| author_facet | Chenlei Li Chenglei Song Kunlong Qi Yingke Liu Yaqing Dou Xiuling Li Ruimin Qiao Kejun Wang Xuelei Han Xinjian Li |
| author_sort | Chenlei Li |
| collection | DOAJ |
| description | The estrus cycle of multiparous Large White sows was divided into three stages to solve the problems of heavy workload and low accuracy of the traditional estrus identification method in pig production. Saliva protein was extracted from the oral saliva of multiparous sows. Label-free quantitative proteomics was used to detect salivary proteome, and MaxQuant software was used for quality control. Results showed that 246 proteins were identified in the three stages, where 40 proteins were significantly different (<i>p</i> < 0.05). The total proteins identified were enriched by STEM software and the protein function was annotated by using the ClueGO plug-in in the Cytoscape software. The results were enriched to eight different trends. The annotated items were related to protein synthesis and processing and estrogen response. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differential proteins involved in the pathways and entries included oocyte meiosis, response to estradiol, and oogenesis. Further interaction analysis showed that an interaction occurred between P00355, F1SHL9, P28491, F1SDR7, F2Z558, F1RYY6, and F2Z5G3 proteins. The findings served as a basis for revealing the changes in salivary protein content in the sow estrus cycle and provided a reference for the development of an estrus identification kit/test strip in the next step. |
| first_indexed | 2024-03-09T22:10:29Z |
| format | Article |
| id | doaj.art-8c2389dbb1634c91ac1f668f6d332cca |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:10:29Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-8c2389dbb1634c91ac1f668f6d332cca2023-11-23T19:32:40ZengMDPI AGAnimals2076-26152022-06-011213165610.3390/ani12131656Identification of Estrus in Sows Based on Salivary ProteomicsChenlei Li0Chenglei Song1Kunlong Qi2Yingke Liu3Yaqing Dou4Xiuling Li5Ruimin Qiao6Kejun Wang7Xuelei Han8Xinjian Li9College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, ChinaThe estrus cycle of multiparous Large White sows was divided into three stages to solve the problems of heavy workload and low accuracy of the traditional estrus identification method in pig production. Saliva protein was extracted from the oral saliva of multiparous sows. Label-free quantitative proteomics was used to detect salivary proteome, and MaxQuant software was used for quality control. Results showed that 246 proteins were identified in the three stages, where 40 proteins were significantly different (<i>p</i> < 0.05). The total proteins identified were enriched by STEM software and the protein function was annotated by using the ClueGO plug-in in the Cytoscape software. The results were enriched to eight different trends. The annotated items were related to protein synthesis and processing and estrogen response. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differential proteins involved in the pathways and entries included oocyte meiosis, response to estradiol, and oogenesis. Further interaction analysis showed that an interaction occurred between P00355, F1SHL9, P28491, F1SDR7, F2Z558, F1RYY6, and F2Z5G3 proteins. The findings served as a basis for revealing the changes in salivary protein content in the sow estrus cycle and provided a reference for the development of an estrus identification kit/test strip in the next step.https://www.mdpi.com/2076-2615/12/13/1656Large White sowestrus identificationsalivary proteomicsprotein interaction |
| spellingShingle | Chenlei Li Chenglei Song Kunlong Qi Yingke Liu Yaqing Dou Xiuling Li Ruimin Qiao Kejun Wang Xuelei Han Xinjian Li Identification of Estrus in Sows Based on Salivary Proteomics Animals Large White sow estrus identification salivary proteomics protein interaction |
| title | Identification of Estrus in Sows Based on Salivary Proteomics |
| title_full | Identification of Estrus in Sows Based on Salivary Proteomics |
| title_fullStr | Identification of Estrus in Sows Based on Salivary Proteomics |
| title_full_unstemmed | Identification of Estrus in Sows Based on Salivary Proteomics |
| title_short | Identification of Estrus in Sows Based on Salivary Proteomics |
| title_sort | identification of estrus in sows based on salivary proteomics |
| topic | Large White sow estrus identification salivary proteomics protein interaction |
| url | https://www.mdpi.com/2076-2615/12/13/1656 |
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