Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales
Migration patterns of humpback whales have been monitored using 316L stainless steel (SS) satellite telemetry tags. The potential for tissue infection and necrosis is increased if the bacteria, naturally a part of the diverse microbiome on the skin of humpback whales, can adhere to and colonize the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2019.00618/full |
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| author | Ariana Tyo Sonja Welch Maureen Hennenfent Pegah Kord Fooroshani Bruce P. Lee Rupak Rajachar |
| author_facet | Ariana Tyo Sonja Welch Maureen Hennenfent Pegah Kord Fooroshani Bruce P. Lee Rupak Rajachar |
| author_sort | Ariana Tyo |
| collection | DOAJ |
| description | Migration patterns of humpback whales have been monitored using 316L stainless steel (SS) satellite telemetry tags. The potential for tissue infection and necrosis is increased if the bacteria, naturally a part of the diverse microbiome on the skin of humpback whales, can adhere to and colonize the surface of the tags. Polydopamine (pDA) has the potential to prevent the adhesion of one of the most prevalent bacterial strains on the surface of the skin of cetaceans (Psychrobacter) through the release of hydrogen peroxide. The release of hydrogen peroxide from the pDA coatings (40–100 μM) has the ability to induce a bacteriostatic response in E. coli, a commonly used bacteria strain in antimicrobial studies and potentially P. cryohalolentis, a common humpback associated bacteria. The H2O2 dose required to induce bacteriostatic conditions in E. coli is approximately 60 μM and in P. cryohalolentis is 100 μM. Bacterial adhesion on the surface of pDA coated SS coupons was measured first using E. coli. The coating successfully prevented adhesion of E. coli on the surface of SS coupons under certain conditions (60% reduction, p < 0.05) but the same was not seen with P. cryohalolentis. When coating conditions were altered (an increase in pH and temperature) the adhesion of P. cryohalolentis was reduced (80% reduction, p < 0.001). Overall, the pDA coatings have the capacity to generate varying amounts of hydrogen peroxide by altering the coating conditions and have the ability to reduce bacterial adhesion on the surface of satellite telemetry tags, and therefore the potential to increase tag functional service lifetime. |
| first_indexed | 2024-12-22T03:42:13Z |
| format | Article |
| id | doaj.art-8c266ff269bb46b4828699a4190452b9 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-22T03:42:13Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-8c266ff269bb46b4828699a4190452b92022-12-21T18:40:13ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-09-01710.3389/fchem.2019.00618473023Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback WhalesAriana Tyo0Sonja Welch1Maureen Hennenfent2Pegah Kord Fooroshani3Bruce P. Lee4Rupak Rajachar5Engineered Biomaterials Lab, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United StatesEngineered Biomaterials Lab, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United StatesEngineered Biomaterials Lab, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United StatesBiomimetics Lab, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United StatesBiomimetics Lab, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United StatesEngineered Biomaterials Lab, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United StatesMigration patterns of humpback whales have been monitored using 316L stainless steel (SS) satellite telemetry tags. The potential for tissue infection and necrosis is increased if the bacteria, naturally a part of the diverse microbiome on the skin of humpback whales, can adhere to and colonize the surface of the tags. Polydopamine (pDA) has the potential to prevent the adhesion of one of the most prevalent bacterial strains on the surface of the skin of cetaceans (Psychrobacter) through the release of hydrogen peroxide. The release of hydrogen peroxide from the pDA coatings (40–100 μM) has the ability to induce a bacteriostatic response in E. coli, a commonly used bacteria strain in antimicrobial studies and potentially P. cryohalolentis, a common humpback associated bacteria. The H2O2 dose required to induce bacteriostatic conditions in E. coli is approximately 60 μM and in P. cryohalolentis is 100 μM. Bacterial adhesion on the surface of pDA coated SS coupons was measured first using E. coli. The coating successfully prevented adhesion of E. coli on the surface of SS coupons under certain conditions (60% reduction, p < 0.05) but the same was not seen with P. cryohalolentis. When coating conditions were altered (an increase in pH and temperature) the adhesion of P. cryohalolentis was reduced (80% reduction, p < 0.001). Overall, the pDA coatings have the capacity to generate varying amounts of hydrogen peroxide by altering the coating conditions and have the ability to reduce bacterial adhesion on the surface of satellite telemetry tags, and therefore the potential to increase tag functional service lifetime.https://www.frontiersin.org/article/10.3389/fchem.2019.00618/fullpolydopamineantimicrobialstainless steelhydrogen peroxidebacteriacoating |
| spellingShingle | Ariana Tyo Sonja Welch Maureen Hennenfent Pegah Kord Fooroshani Bruce P. Lee Rupak Rajachar Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales Frontiers in Chemistry polydopamine antimicrobial stainless steel hydrogen peroxide bacteria coating |
| title | Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales |
| title_full | Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales |
| title_fullStr | Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales |
| title_full_unstemmed | Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales |
| title_short | Development and Characterization of an Antimicrobial Polydopamine Coating for Conservation of Humpback Whales |
| title_sort | development and characterization of an antimicrobial polydopamine coating for conservation of humpback whales |
| topic | polydopamine antimicrobial stainless steel hydrogen peroxide bacteria coating |
| url | https://www.frontiersin.org/article/10.3389/fchem.2019.00618/full |
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