Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus
Summary: The pinewood nematode Bursaphelenchus xylophilus is an invasive and destructive pathogen in forestry. Serratia marcescens AHPC29 was previously found to have nematicidal activity on B. xylophilus. The effect of AHPC29 growth temperature on B. xylophilus inhibition is unknown. Here we show t...
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Elsevier
2023-07-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223011598 |
| _version_ | 1797774558530371584 |
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| author | Yanfen Zhang Xiaoting Gu Chenying Huang Jiao Zhou Juan Shi Lilin Zhao |
| author_facet | Yanfen Zhang Xiaoting Gu Chenying Huang Jiao Zhou Juan Shi Lilin Zhao |
| author_sort | Yanfen Zhang |
| collection | DOAJ |
| description | Summary: The pinewood nematode Bursaphelenchus xylophilus is an invasive and destructive pathogen in forestry. Serratia marcescens AHPC29 was previously found to have nematicidal activity on B. xylophilus. The effect of AHPC29 growth temperature on B. xylophilus inhibition is unknown. Here we show that AHPC29 cultured at 15°C or 25°C, but not 37°C, inhibited B. xylophilus reproduction. Metabolomic analysis found 31 up-regulated metabolites as potential effective substances in this temperature-related difference, with five of them were tested to be effective in inhibiting B. xylophilus reproduction. Among the five metabolites, salsolinol was further verified in bacterial cultures with effective inhibition concentrations. This study found the inhibition of S. marcescens AHPC29 on B. xylophilus reproduction was temperature regulated and the differently expressed metabolites salsolinol played roles in this temperature-regulated effect, which implies the capability of S. marcescens and its metabolites as promising new agents for the management of B. xylophilus. |
| first_indexed | 2024-03-12T22:22:48Z |
| format | Article |
| id | doaj.art-8c2852722c1943e882147a5f1e170c8f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:48Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
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| spelling | doaj.art-8c2852722c1943e882147a5f1e170c8f2023-07-23T04:55:28ZengElsevieriScience2589-00422023-07-01267107082Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilusYanfen Zhang0Xiaoting Gu1Chenying Huang2Jiao Zhou3Juan Shi4Lilin Zhao5State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, ChinaSino-France Joint Laboratory for Invasive Forest Pests in Eurasia, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, ChinaSino-France Joint Laboratory for Invasive Forest Pests in Eurasia, College of Forestry, Beijing Forestry University, Beijing 100083, China; Corresponding authorState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authorSummary: The pinewood nematode Bursaphelenchus xylophilus is an invasive and destructive pathogen in forestry. Serratia marcescens AHPC29 was previously found to have nematicidal activity on B. xylophilus. The effect of AHPC29 growth temperature on B. xylophilus inhibition is unknown. Here we show that AHPC29 cultured at 15°C or 25°C, but not 37°C, inhibited B. xylophilus reproduction. Metabolomic analysis found 31 up-regulated metabolites as potential effective substances in this temperature-related difference, with five of them were tested to be effective in inhibiting B. xylophilus reproduction. Among the five metabolites, salsolinol was further verified in bacterial cultures with effective inhibition concentrations. This study found the inhibition of S. marcescens AHPC29 on B. xylophilus reproduction was temperature regulated and the differently expressed metabolites salsolinol played roles in this temperature-regulated effect, which implies the capability of S. marcescens and its metabolites as promising new agents for the management of B. xylophilus.http://www.sciencedirect.com/science/article/pii/S2589004223011598IochemistryBiological sciencesEvolutionary biology |
| spellingShingle | Yanfen Zhang Xiaoting Gu Chenying Huang Jiao Zhou Juan Shi Lilin Zhao Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus iScience Iochemistry Biological sciences Evolutionary biology |
| title | Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus |
| title_full | Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus |
| title_fullStr | Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus |
| title_full_unstemmed | Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus |
| title_short | Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus |
| title_sort | temperature regulated metabolites of serratia marcescens inhibited reproduction of pinewood nematode bursaphelenchus xylophilus |
| topic | Iochemistry Biological sciences Evolutionary biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223011598 |
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