Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts
AIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.<p>METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescenc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2017-09-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2017/9/201709008.pdf |
| Summary: | AIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.<p>METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics. The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl. The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction(real time-qPCR)and the protein expression was detected with Western blot. <p>RESULTS:The cultured HTFs expressed TGF-β and CTGF. The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(<i>t</i>=20.042, 14.995, <i>P<</i>0.05). the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(<i>t</i>=46.058、12.452, <i>P</i><0.05)<p>CONCLUSION: The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level. LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level. LiCl has the potential to modulate wound healing for glaucoma filtration surgery. |
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| ISSN: | 1672-5123 1672-5123 |