Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts
AIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.<p>METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescenc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2017-09-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2017/9/201709008.pdf |
| _version_ | 1818155394319515648 |
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| author | Su-Su Lu Shan-Shan Liu Xiao-Jun Fan Xiao-Xiang Sun Jiang-Hua Bian Ji-Bing Wang |
| author_facet | Su-Su Lu Shan-Shan Liu Xiao-Jun Fan Xiao-Xiang Sun Jiang-Hua Bian Ji-Bing Wang |
| author_sort | Su-Su Lu |
| collection | DOAJ |
| description | AIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.<p>METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics. The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl. The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction(real time-qPCR)and the protein expression was detected with Western blot. <p>RESULTS:The cultured HTFs expressed TGF-β and CTGF. The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(<i>t</i>=20.042, 14.995, <i>P<</i>0.05). the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(<i>t</i>=46.058、12.452, <i>P</i><0.05)<p>CONCLUSION: The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level. LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level. LiCl has the potential to modulate wound healing for glaucoma filtration surgery. |
| first_indexed | 2024-12-11T14:41:42Z |
| format | Article |
| id | doaj.art-8c2926d77a4544de8044d03bced2fe5b |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-11T14:41:42Z |
| publishDate | 2017-09-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-8c2926d77a4544de8044d03bced2fe5b2022-12-22T01:01:53ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232017-09-011791639164210.3980/j.issn.1672-5123.2017.9.08Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblastsSu-Su Lu0Shan-Shan Liu1Xiao-Jun Fan2Xiao-Xiang Sun3Jiang-Hua Bian4Ji-Bing Wang5Clinical College of Weifang Medical University, Weifang 261000, Shandong Province, ChinaWeifang Eye Hospital, Weifang 261041, Shandong Province, ChinaWeifang Eye Hospital, Weifang 261041, Shandong Province, ChinaClinical College of Weifang Medical University, Weifang 261000, Shandong Province, ChinaClinical College of Weifang Medical University, Weifang 261000, Shandong Province, ChinaWeifang Eye Hospital, Weifang 261041, Shandong Province, ChinaAIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.<p>METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics. The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl. The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction(real time-qPCR)and the protein expression was detected with Western blot. <p>RESULTS:The cultured HTFs expressed TGF-β and CTGF. The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(<i>t</i>=20.042, 14.995, <i>P<</i>0.05). the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(<i>t</i>=46.058、12.452, <i>P</i><0.05)<p>CONCLUSION: The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level. LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level. LiCl has the potential to modulate wound healing for glaucoma filtration surgery.http://ies.ijo.cn/cn_publish/2017/9/201709008.pdftransforming growth factor betaconnective tissue growth factorTenon's capsulefibroblastslithium chloridelithium chloride |
| spellingShingle | Su-Su Lu Shan-Shan Liu Xiao-Jun Fan Xiao-Xiang Sun Jiang-Hua Bian Ji-Bing Wang Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts Guoji Yanke Zazhi transforming growth factor beta connective tissue growth factor Tenon's capsule fibroblasts lithium chloride lithium chloride |
| title | Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts |
| title_full | Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts |
| title_fullStr | Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts |
| title_full_unstemmed | Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts |
| title_short | Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts |
| title_sort | effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human tenon s capsule fibroblasts |
| topic | transforming growth factor beta connective tissue growth factor Tenon's capsule fibroblasts lithium chloride lithium chloride |
| url | http://ies.ijo.cn/cn_publish/2017/9/201709008.pdf |
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