The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection
The most used and reliable indicator of equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for the detection of EIAV antibodies as well as the potential advantages of their us...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
2015-03-01
|
| Series: | Revista Argentina de Microbiología |
| Subjects: | |
| Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412015000100005&lng=en&tlng=en |
| _version_ | 1818453335336812544 |
|---|---|
| author | Irene Alvarez Fabiana Cipolini Andrés Wigdorovitz Karina Trono Maria E Barrandeguy |
| author_facet | Irene Alvarez Fabiana Cipolini Andrés Wigdorovitz Karina Trono Maria E Barrandeguy |
| author_sort | Irene Alvarez |
| collection | DOAJ |
| description | The most used and reliable indicator of equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for the detection of EIAV antibodies as well as the potential advantages of their use as an EIAV infection screening tool were evaluated in 302 horse serum samples. Both ELISA assays showed 100% diagnostic sensitivity, and 92.3-94.3% diagnostic specificity. Discordant results were analyzed by immunoblot. The results showed that both ELISA tests are very efficient at detecting EIAV infected animals, allowing to identify a higher number of positive horse cases. Thus, ELISA assays can be useful tools in EIA control and eradication. |
| first_indexed | 2024-12-14T21:37:21Z |
| format | Article |
| id | doaj.art-8c2c022b4f104fff9cb71d7df4793bab |
| institution | Directory Open Access Journal |
| issn | 1851-7617 |
| language | English |
| last_indexed | 2024-12-14T21:37:21Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Revista Argentina de Microbiología |
| spelling | doaj.art-8c2c022b4f104fff9cb71d7df4793bab2022-12-21T22:46:33ZengElsevier EspañaRevista Argentina de Microbiología1851-76172015-03-01471252810.1016/j.ram.2014.12.001S0325-75412015000100005The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infectionIrene Alvarez0Fabiana Cipolini1Andrés Wigdorovitz2Karina Trono3Maria E Barrandeguy4INTAUniversidad Nacional del NordesteINTAINTAINTAThe most used and reliable indicator of equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for the detection of EIAV antibodies as well as the potential advantages of their use as an EIAV infection screening tool were evaluated in 302 horse serum samples. Both ELISA assays showed 100% diagnostic sensitivity, and 92.3-94.3% diagnostic specificity. Discordant results were analyzed by immunoblot. The results showed that both ELISA tests are very efficient at detecting EIAV infected animals, allowing to identify a higher number of positive horse cases. Thus, ELISA assays can be useful tools in EIA control and eradication.http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412015000100005&lng=en&tlng=enElisaEquine infectious anemiaDiagnosisElisa. Virus de la anemia infecciosa equina. Diagnóstico |
| spellingShingle | Irene Alvarez Fabiana Cipolini Andrés Wigdorovitz Karina Trono Maria E Barrandeguy The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection Revista Argentina de Microbiología Elisa Equine infectious anemia Diagnosis Elisa. Virus de la anemia infecciosa equina. Diagnóstico |
| title | The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection |
| title_full | The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection |
| title_fullStr | The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection |
| title_full_unstemmed | The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection |
| title_short | The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection |
| title_sort | efficacy of elisa commercial kits for the screening of equine infectious anemia virus infection |
| topic | Elisa Equine infectious anemia Diagnosis Elisa. Virus de la anemia infecciosa equina. Diagnóstico |
| url | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412015000100005&lng=en&tlng=en |
| work_keys_str_mv | AT irenealvarez theefficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT fabianacipolini theefficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT andreswigdorovitz theefficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT karinatrono theefficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT mariaebarrandeguy theefficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT irenealvarez efficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT fabianacipolini efficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT andreswigdorovitz efficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT karinatrono efficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection AT mariaebarrandeguy efficacyofelisacommercialkitsforthescreeningofequineinfectiousanemiavirusinfection |