Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System
The Liposomal Encapsulation of Polysaccharides (LEPS) dual antigen vaccine carrier system was assessed across two distinct polysaccharides for encapsulation efficiency, subsequent liposomal surface adornment with protein, adjuvant addition, and size and charge metrics. The polysaccharides derive fro...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/15/3320 |
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| author | Roozbeh Nayerhoda Dongwon Park Charles Jones Elsa N. Bou Ghanem Blaine A. Pfeifer |
| author_facet | Roozbeh Nayerhoda Dongwon Park Charles Jones Elsa N. Bou Ghanem Blaine A. Pfeifer |
| author_sort | Roozbeh Nayerhoda |
| collection | DOAJ |
| description | The Liposomal Encapsulation of Polysaccharides (LEPS) dual antigen vaccine carrier system was assessed across two distinct polysaccharides for encapsulation efficiency, subsequent liposomal surface adornment with protein, adjuvant addition, and size and charge metrics. The polysaccharides derive from two different serotypes of <i>Streptococcus pneumoniae</i> and have traditionally served as the active ingredients of vaccines against pneumococcal disease. The LEPS system was designed to mimic glycoconjugate vaccines that covalently couple polysaccharides to protein carriers; however, the LEPS system uses a noncovalent co-localization mechanism through protein liposomal surface attachment. In an effort to more thoroughly characterize the LEPS system across individual vaccine components and thus support broader future utility, polysaccharides from <i>S. pneumoniae</i> serotypes 3 and 4 were systematically compared within the LEPS framework both pre- and post-surface protein attachment. For both polysaccharides, ≥85% encapsulation efficiency was achieved prior to protein surface attachment. Upon protein attachment with either a model protein (GFP) or a pneumococcal disease antigen (PncO), polysaccharide encapsulation was maintained at ≥61% encapsulation efficiency. Final LEPS carriers were also evaluated with and without alum as an included adjuvant, with encapsulation efficiency maintained at ≥30%, while protein surface attachment efficiency was maintained at ≥~50%. Finally, similar trends and distributions were observed across the different polysaccharides when assessed for liposomal zeta potential and size. |
| first_indexed | 2024-03-10T18:12:11Z |
| format | Article |
| id | doaj.art-8c2d20b5c79145a2892b040f716e696a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T18:12:11Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-8c2d20b5c79145a2892b040f716e696a2023-11-20T07:59:59ZengMDPI AGMaterials1996-19442020-07-011315332010.3390/ma13153320Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides SystemRoozbeh Nayerhoda0Dongwon Park1Charles Jones2Elsa N. Bou Ghanem3Blaine A. Pfeifer4Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USADepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USAAbcombi Biosciences Inc., 1576 Sweet Home Road, Amherst, NY 14260, USADepartment of Microbiology and Immunology, University at Buffalo, The State University of New York, Buffalo, NY 14260, USADepartment of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USAThe Liposomal Encapsulation of Polysaccharides (LEPS) dual antigen vaccine carrier system was assessed across two distinct polysaccharides for encapsulation efficiency, subsequent liposomal surface adornment with protein, adjuvant addition, and size and charge metrics. The polysaccharides derive from two different serotypes of <i>Streptococcus pneumoniae</i> and have traditionally served as the active ingredients of vaccines against pneumococcal disease. The LEPS system was designed to mimic glycoconjugate vaccines that covalently couple polysaccharides to protein carriers; however, the LEPS system uses a noncovalent co-localization mechanism through protein liposomal surface attachment. In an effort to more thoroughly characterize the LEPS system across individual vaccine components and thus support broader future utility, polysaccharides from <i>S. pneumoniae</i> serotypes 3 and 4 were systematically compared within the LEPS framework both pre- and post-surface protein attachment. For both polysaccharides, ≥85% encapsulation efficiency was achieved prior to protein surface attachment. Upon protein attachment with either a model protein (GFP) or a pneumococcal disease antigen (PncO), polysaccharide encapsulation was maintained at ≥61% encapsulation efficiency. Final LEPS carriers were also evaluated with and without alum as an included adjuvant, with encapsulation efficiency maintained at ≥30%, while protein surface attachment efficiency was maintained at ≥~50%. Finally, similar trends and distributions were observed across the different polysaccharides when assessed for liposomal zeta potential and size.https://www.mdpi.com/1996-1944/13/15/3320liposomeserotypepolysaccharide<i>Streptococcus pneumoniae</i>vaccinepneumococcal disease |
| spellingShingle | Roozbeh Nayerhoda Dongwon Park Charles Jones Elsa N. Bou Ghanem Blaine A. Pfeifer Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System Materials liposome serotype polysaccharide <i>Streptococcus pneumoniae</i> vaccine pneumococcal disease |
| title | Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System |
| title_full | Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System |
| title_fullStr | Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System |
| title_full_unstemmed | Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System |
| title_short | Extended Polysaccharide Analysis within the Liposomal Encapsulation of Polysaccharides System |
| title_sort | extended polysaccharide analysis within the liposomal encapsulation of polysaccharides system |
| topic | liposome serotype polysaccharide <i>Streptococcus pneumoniae</i> vaccine pneumococcal disease |
| url | https://www.mdpi.com/1996-1944/13/15/3320 |
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