Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly
Human cells are continuously exposed to various stress factors in their physiological environment. Evidence suggests that certain mechanical stress can affect cell cycle progression and cell proliferation. However, the signaling pathways involved in this process are not well understood. To investiga...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300019S.pdf |
| _version_ | 1797393743679062016 |
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| author | Son Gwang-ic Lee Eunil Kim Mari Lee Seoeun Moon Yesol Kim Joonhee |
| author_facet | Son Gwang-ic Lee Eunil Kim Mari Lee Seoeun Moon Yesol Kim Joonhee |
| author_sort | Son Gwang-ic |
| collection | DOAJ |
| description | Human cells are continuously exposed to various stress factors in their physiological environment. Evidence suggests that certain mechanical stress can affect cell cycle progression and cell proliferation. However, the signaling pathways involved in this process are not well understood. To investigate this, we developed a pressure chamber capable of producing an elevated barometric pressure (EBP) environment of 2×atmospheric absolute pressure (ATA). We then studied the effect of EBP on cell proliferation and its underlying mechanism. Our results show that EBP inhibited cell proliferation by delaying the G2/M phase. Specifically, EBP reduced the expression levels of cell adhesion-related genes and downregulated integrin subunit genes, resulting in weaker interaction between cells and extracellular matrix proteins. In addition, Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division control protein 42 homolog (Cdc42) activity was suppressed, and actin assembly was decreased. These findings suggest that the EBP-mediated G2/M phase delay is due to attenuated cell adhesion and actin cytoskeleton assembly, leading to the inhibition of cell proliferation. Our results provide a crucial molecular mechanism for how certain pressure (changes) can negatively regulate cell proliferation. These findings could potentially be used in the future to develop a pressure therapy to inhibit cell proliferation in cancer patients. |
| first_indexed | 2024-03-09T00:07:19Z |
| format | Article |
| id | doaj.art-8c2d6e1e92444a928a072bd6135c705d |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-09T00:07:19Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-8c2d6e1e92444a928a072bd6135c705d2023-12-12T13:06:42ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175223724510.2298/ABS230313019S0354-46642300019SElevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assemblySon Gwang-ic0Lee Eunil1Kim Mari2Lee Seoeun3Moon Yesol4Kim Joonhee5Department of Preventive Medicine, College of Medicine, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea + Department of Medical Science Graduate School, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of KoreaDepartment of Preventive Medicine, College of Medicine, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea + Department of Medical Science Graduate School, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of KoreaDepartment of Preventive Medicine, College of Medicine, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea + Department of Medical Science Graduate School, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of KoreaDepartment of Preventive Medicine, College of Medicine, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea + Department of Medical Science Graduate School, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of KoreaDepartment of Preventive Medicine, College of Medicine, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea + Department of Medical Science Graduate School, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of KoreaDepartment of Preventive Medicine, College of Medicine, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea + Department of Medical Science Graduate School, Korea University, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of KoreaHuman cells are continuously exposed to various stress factors in their physiological environment. Evidence suggests that certain mechanical stress can affect cell cycle progression and cell proliferation. However, the signaling pathways involved in this process are not well understood. To investigate this, we developed a pressure chamber capable of producing an elevated barometric pressure (EBP) environment of 2×atmospheric absolute pressure (ATA). We then studied the effect of EBP on cell proliferation and its underlying mechanism. Our results show that EBP inhibited cell proliferation by delaying the G2/M phase. Specifically, EBP reduced the expression levels of cell adhesion-related genes and downregulated integrin subunit genes, resulting in weaker interaction between cells and extracellular matrix proteins. In addition, Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division control protein 42 homolog (Cdc42) activity was suppressed, and actin assembly was decreased. These findings suggest that the EBP-mediated G2/M phase delay is due to attenuated cell adhesion and actin cytoskeleton assembly, leading to the inhibition of cell proliferation. Our results provide a crucial molecular mechanism for how certain pressure (changes) can negatively regulate cell proliferation. These findings could potentially be used in the future to develop a pressure therapy to inhibit cell proliferation in cancer patients.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300019S.pdfelevated barometric pressurecell proliferationcell cyclecell adhesionactins |
| spellingShingle | Son Gwang-ic Lee Eunil Kim Mari Lee Seoeun Moon Yesol Kim Joonhee Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly Archives of Biological Sciences elevated barometric pressure cell proliferation cell cycle cell adhesion actins |
| title | Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly |
| title_full | Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly |
| title_fullStr | Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly |
| title_full_unstemmed | Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly |
| title_short | Elevated barometric pressure suppresses cell proliferation by delaying the G2/M phase and weakening integrin-mediated cell adhesion and actin assembly |
| title_sort | elevated barometric pressure suppresses cell proliferation by delaying the g2 m phase and weakening integrin mediated cell adhesion and actin assembly |
| topic | elevated barometric pressure cell proliferation cell cycle cell adhesion actins |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300019S.pdf |
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