Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing
Late spring frost is an important meteorological factor threatening the safe production of winter wheat in China. The young ear is the most vulnerable organ of the wheat plant to spring frost. To gain an insight into the mechanisms underpinning young wheat ears’ tolerance to freezing, we performed a...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15892 |
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| author | Weiling Wang Yuting Zhang Chang Liu Yongwen Dong Xue Jiang Can Zhao Guohui Li Ke Xu Zhongyang Huo |
| author_facet | Weiling Wang Yuting Zhang Chang Liu Yongwen Dong Xue Jiang Can Zhao Guohui Li Ke Xu Zhongyang Huo |
| author_sort | Weiling Wang |
| collection | DOAJ |
| description | Late spring frost is an important meteorological factor threatening the safe production of winter wheat in China. The young ear is the most vulnerable organ of the wheat plant to spring frost. To gain an insight into the mechanisms underpinning young wheat ears’ tolerance to freezing, we performed a comparative proteome analysis of wheat varieties Xumai33 (XM33, freezing-sensitive) and Jimai22 (JM22, freezing-tolerant) under normal and freezing conditions using label-free quantitative proteomic techniques during the anther connective tissue formation phase (ACFP). Under freezing stress, 392 and 103 differently expressed proteins (DEPs) were identified in the young ears of XM33 and JM22, respectively, and among these, 30 proteins were common in both varieties. A functional characterization analysis revealed that these DEPs were associated with antioxidant capacity, cell wall modification, protein folding, dehydration response, and plant–pathogen interactions. The young ears of JM22 showed significantly higher expression levels of antioxidant enzymes, heat shock proteins, and dehydrin under normal conditions compared to those of XM33, which might help to prepare the young ears of JM22 for freezing stress. Our results lead to new insights into understanding the mechanisms in young wheat ears’ response to freezing stress and provide pivotal potential candidate proteins required for improving young wheat ears’ tolerance to spring frost. |
| first_indexed | 2024-03-11T11:27:30Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:27:30Z |
| publishDate | 2023-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8c328e4a964248fc8832913711cff4972023-11-10T15:05:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124211589210.3390/ijms242115892Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring FreezingWeiling Wang0Yuting Zhang1Chang Liu2Yongwen Dong3Xue Jiang4Can Zhao5Guohui Li6Ke Xu7Zhongyang Huo8Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, ChinaLate spring frost is an important meteorological factor threatening the safe production of winter wheat in China. The young ear is the most vulnerable organ of the wheat plant to spring frost. To gain an insight into the mechanisms underpinning young wheat ears’ tolerance to freezing, we performed a comparative proteome analysis of wheat varieties Xumai33 (XM33, freezing-sensitive) and Jimai22 (JM22, freezing-tolerant) under normal and freezing conditions using label-free quantitative proteomic techniques during the anther connective tissue formation phase (ACFP). Under freezing stress, 392 and 103 differently expressed proteins (DEPs) were identified in the young ears of XM33 and JM22, respectively, and among these, 30 proteins were common in both varieties. A functional characterization analysis revealed that these DEPs were associated with antioxidant capacity, cell wall modification, protein folding, dehydration response, and plant–pathogen interactions. The young ears of JM22 showed significantly higher expression levels of antioxidant enzymes, heat shock proteins, and dehydrin under normal conditions compared to those of XM33, which might help to prepare the young ears of JM22 for freezing stress. Our results lead to new insights into understanding the mechanisms in young wheat ears’ response to freezing stress and provide pivotal potential candidate proteins required for improving young wheat ears’ tolerance to spring frost.https://www.mdpi.com/1422-0067/24/21/15892antioxidant capacitycell wall modificationheat shock proteindehydrindefensin |
| spellingShingle | Weiling Wang Yuting Zhang Chang Liu Yongwen Dong Xue Jiang Can Zhao Guohui Li Ke Xu Zhongyang Huo Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing International Journal of Molecular Sciences antioxidant capacity cell wall modification heat shock protein dehydrin defensin |
| title | Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing |
| title_full | Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing |
| title_fullStr | Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing |
| title_full_unstemmed | Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing |
| title_short | Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (<i>Triticum aestivum</i> L.) Ears’ Response to Spring Freezing |
| title_sort | label free quantitative proteomics reveal the mechanisms of young wheat i triticum aestivum i l ears response to spring freezing |
| topic | antioxidant capacity cell wall modification heat shock protein dehydrin defensin |
| url | https://www.mdpi.com/1422-0067/24/21/15892 |
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