Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer
Abstract We report a new approach to controllable thermal stimulation of a single living cell and its compartments. The technique is based on the use of a single polycrystalline diamond particle containing silicon-vacancy (SiV) color centers. Due to the presence of amorphous carbon at its intercryst...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35141-4 |
| _version_ | 1797817992893956096 |
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| author | Alexey M. Romshin Vadim Zeeb Evgenii Glushkov Aleksandra Radenovic Andrey G. Sinogeikin Igor I. Vlasov |
| author_facet | Alexey M. Romshin Vadim Zeeb Evgenii Glushkov Aleksandra Radenovic Andrey G. Sinogeikin Igor I. Vlasov |
| author_sort | Alexey M. Romshin |
| collection | DOAJ |
| description | Abstract We report a new approach to controllable thermal stimulation of a single living cell and its compartments. The technique is based on the use of a single polycrystalline diamond particle containing silicon-vacancy (SiV) color centers. Due to the presence of amorphous carbon at its intercrystalline boundaries, such a particle is an efficient light absorber and becomes a local heat source when illuminated by a laser. Furthermore, the temperature of such a local heater is tracked by the spectral shift of the zero-phonon line of SiV centers. Thus, the diamond particle acts simultaneously as a heater and a thermometer. In the current work, we demonstrate the ability of such a Diamond Heater-Thermometer (DHT) to locally alter the temperature, one of the numerous parameters that play a decisive role for the living organisms at the nanoscale. In particular, we show that the local heating of 11–12 °C relative to the ambient temperature (22 °C) next to individual HeLa cells and neurons, isolated from the mouse hippocampus, leads to a change in the intracellular distribution of the concentration of free calcium ions. For individual HeLa cells, a long-term (about 30 s) increase in the integral intensity of Fluo-4 NW fluorescence by about three times is observed, which characterizes an increase in the [Ca2+]cyt concentration of free calcium in the cytoplasm. Heating near mouse hippocampal neurons also caused a calcium surge—an increase in the intensity of Fluo-4 NW fluorescence by 30% and a duration of ~ 0.4 ms. |
| first_indexed | 2024-03-13T09:02:48Z |
| format | Article |
| id | doaj.art-8c341688001240a88c7bc9cbc509f359 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:02:48Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8c341688001240a88c7bc9cbc509f3592023-05-28T11:15:58ZengNature PortfolioScientific Reports2045-23222023-05-011311710.1038/s41598-023-35141-4Nanoscale thermal control of a single living cell enabled by diamond heater-thermometerAlexey M. Romshin0Vadim Zeeb1Evgenii Glushkov2Aleksandra Radenovic3Andrey G. Sinogeikin4Igor I. Vlasov5Prokhorov General Physics Institute of the Russian Academy of SciencesInstitute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesLaboratory of Nanoscale Biology, Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL)Laboratory of Nanoscale Biology, Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL)NanThermix SA, Ecole Polytechnique Federale de Lausanne (EPFL) Innovation ParkProkhorov General Physics Institute of the Russian Academy of SciencesAbstract We report a new approach to controllable thermal stimulation of a single living cell and its compartments. The technique is based on the use of a single polycrystalline diamond particle containing silicon-vacancy (SiV) color centers. Due to the presence of amorphous carbon at its intercrystalline boundaries, such a particle is an efficient light absorber and becomes a local heat source when illuminated by a laser. Furthermore, the temperature of such a local heater is tracked by the spectral shift of the zero-phonon line of SiV centers. Thus, the diamond particle acts simultaneously as a heater and a thermometer. In the current work, we demonstrate the ability of such a Diamond Heater-Thermometer (DHT) to locally alter the temperature, one of the numerous parameters that play a decisive role for the living organisms at the nanoscale. In particular, we show that the local heating of 11–12 °C relative to the ambient temperature (22 °C) next to individual HeLa cells and neurons, isolated from the mouse hippocampus, leads to a change in the intracellular distribution of the concentration of free calcium ions. For individual HeLa cells, a long-term (about 30 s) increase in the integral intensity of Fluo-4 NW fluorescence by about three times is observed, which characterizes an increase in the [Ca2+]cyt concentration of free calcium in the cytoplasm. Heating near mouse hippocampal neurons also caused a calcium surge—an increase in the intensity of Fluo-4 NW fluorescence by 30% and a duration of ~ 0.4 ms.https://doi.org/10.1038/s41598-023-35141-4 |
| spellingShingle | Alexey M. Romshin Vadim Zeeb Evgenii Glushkov Aleksandra Radenovic Andrey G. Sinogeikin Igor I. Vlasov Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer Scientific Reports |
| title | Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer |
| title_full | Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer |
| title_fullStr | Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer |
| title_full_unstemmed | Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer |
| title_short | Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer |
| title_sort | nanoscale thermal control of a single living cell enabled by diamond heater thermometer |
| url | https://doi.org/10.1038/s41598-023-35141-4 |
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