Effects of microcystin-LR in isolated perfused rat kidney
Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1999-08-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008 |
| _version_ | 1818588995812065280 |
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| author | A.C.L. Nobre M.C.M. Jorge D.B. Menezes M.C. Fonteles H.S.A. Monteiro |
| author_facet | A.C.L. Nobre M.C.M. Jorge D.B. Menezes M.C. Fonteles H.S.A. Monteiro |
| author_sort | A.C.L. Nobre |
| collection | DOAJ |
| description | Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P<0.05). At 90 min there was a significant increase in perfusate pressure (C = 129.7 ± 4.81 and T = 175.0 ± 1.15 mmHg) and glomerular filtration rate (C = 0.66 ± 0.07 and T = 1.10 ± 0.04 ml g-1 min-1) and there was a significant reduction in fractional sodium tubular transport at 120 min (C = 78.6 ± 0.98 and T = 73.9 ± 0.95%). Histopathologic analysis of the perfused kidneys showed protein material in the urinary space, suggestive of renal toxicity. These data demonstrate renal vascular, glomerular and urinary effects of microcystin-LR, indicating that microcystin acts directly on the kidney by probable inhibition of protein phosphatases. |
| first_indexed | 2024-12-16T09:33:37Z |
| format | Article |
| id | doaj.art-8c3422c582d8433980953fbbaf8fafed |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-16T09:33:37Z |
| publishDate | 1999-08-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-8c3422c582d8433980953fbbaf8fafed2022-12-21T22:36:27ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1999-08-0132898598810.1590/S0100-879X1999000800008Effects of microcystin-LR in isolated perfused rat kidneyA.C.L. NobreM.C.M. JorgeD.B. MenezesM.C. FontelesH.S.A. MonteiroMicrocystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P<0.05). At 90 min there was a significant increase in perfusate pressure (C = 129.7 ± 4.81 and T = 175.0 ± 1.15 mmHg) and glomerular filtration rate (C = 0.66 ± 0.07 and T = 1.10 ± 0.04 ml g-1 min-1) and there was a significant reduction in fractional sodium tubular transport at 120 min (C = 78.6 ± 0.98 and T = 73.9 ± 0.95%). Histopathologic analysis of the perfused kidneys showed protein material in the urinary space, suggestive of renal toxicity. These data demonstrate renal vascular, glomerular and urinary effects of microcystin-LR, indicating that microcystin acts directly on the kidney by probable inhibition of protein phosphatases.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008microcystin-LRkidneyperfused kidney |
| spellingShingle | A.C.L. Nobre M.C.M. Jorge D.B. Menezes M.C. Fonteles H.S.A. Monteiro Effects of microcystin-LR in isolated perfused rat kidney Brazilian Journal of Medical and Biological Research microcystin-LR kidney perfused kidney |
| title | Effects of microcystin-LR in isolated perfused rat kidney |
| title_full | Effects of microcystin-LR in isolated perfused rat kidney |
| title_fullStr | Effects of microcystin-LR in isolated perfused rat kidney |
| title_full_unstemmed | Effects of microcystin-LR in isolated perfused rat kidney |
| title_short | Effects of microcystin-LR in isolated perfused rat kidney |
| title_sort | effects of microcystin lr in isolated perfused rat kidney |
| topic | microcystin-LR kidney perfused kidney |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008 |
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