The proteomic landscape shows oncologic relevance in cystitis glandularis
Background The relationship between cystitis glandularis (CG) and bladder malignancy remains unclear. Methods We identified the oncologic significance of CG at the molecular level using liquid chromatography-tandem mass spectrometry-based proteomic analysis of 10 CG, 12 urothelial carcinoma (UC), an...
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| Format: | Article |
| Language: | English |
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Korean Society of Pathologists & the Korean Society for Cytopathology
2023-01-01
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| Series: | Journal of Pathology and Translational Medicine |
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| Online Access: | http://www.jpatholtm.org/upload/pdf/jptm-2022-10-24.pdf |
| _version_ | 1797951387067219968 |
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| author | Jun Yong Kim Dohyun Han Hyeyoon Kim Minsun Jung Han Suk Ryu |
| author_facet | Jun Yong Kim Dohyun Han Hyeyoon Kim Minsun Jung Han Suk Ryu |
| author_sort | Jun Yong Kim |
| collection | DOAJ |
| description | Background The relationship between cystitis glandularis (CG) and bladder malignancy remains unclear. Methods We identified the oncologic significance of CG at the molecular level using liquid chromatography-tandem mass spectrometry-based proteomic analysis of 10 CG, 12 urothelial carcinoma (UC), and nine normal urothelium (NU) specimens. Differentially expressed proteins (DEPs) were identified based on an analysis of variance false discovery rate < 0.05, and their functional enrichment was analyzed using a network model, Gene Set Enrichment Analysis, and Gene Ontology annotation. Results We identified 9,890 proteins across all samples and 1,139 DEPs among the three entities. A substantial number of DEPs overlapped in CG/NU, distinct from UC. Interestingly, we found that a subset of DEP clusters (n = 53, 5%) was differentially expressed in NU but similarly between CG and UC. This “UC-like signature” was enriched for reactive oxygen species (ROS) and energy metabolism, growth and DNA repair, transport, motility, epithelial-mesenchymal transition, and cell survival. Using the top 10 shortlisted DEPs, including SOD2, PRKCD, CYCS, and HCLS1, we identified functional elements related to ROS metabolism, development, and transport using network analysis. The abundance of these four molecules in UC/CG than in NU was consistent with the oncologic functions in CG. Conclusions Using a proteomic approach, we identified a predominantly non-neoplastic landscape of CG, which was closer to NU than to UC. We also confirmed a small subset of common DEPs in UC and CG, suggesting that altered ROS metabolism might imply potential cancerous risks in CG. |
| first_indexed | 2024-04-10T22:29:48Z |
| format | Article |
| id | doaj.art-8c36fbeea8c141d2b7f6b23ca2372f0b |
| institution | Directory Open Access Journal |
| issn | 2383-7837 2383-7845 |
| language | English |
| last_indexed | 2024-04-10T22:29:48Z |
| publishDate | 2023-01-01 |
| publisher | Korean Society of Pathologists & the Korean Society for Cytopathology |
| record_format | Article |
| series | Journal of Pathology and Translational Medicine |
| spelling | doaj.art-8c36fbeea8c141d2b7f6b23ca2372f0b2023-01-17T06:08:50ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452023-01-01571677410.4132/jptm.2022.10.2417052The proteomic landscape shows oncologic relevance in cystitis glandularisJun Yong Kim0Dohyun Han1Hyeyoon Kim2Minsun Jung3Han Suk Ryu4 Department of Pathology, Yonsei University College of Medicine, Seoul, Korea Transdisciplinary Department of Medicine and Advanced Technology, Seoul National University Hospital, Seoul, Korea Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea Department of Pathology, Yonsei University College of Medicine, Seoul, Korea Department of Pathology, Seoul National University College of Medicine, Seoul, KoreaBackground The relationship between cystitis glandularis (CG) and bladder malignancy remains unclear. Methods We identified the oncologic significance of CG at the molecular level using liquid chromatography-tandem mass spectrometry-based proteomic analysis of 10 CG, 12 urothelial carcinoma (UC), and nine normal urothelium (NU) specimens. Differentially expressed proteins (DEPs) were identified based on an analysis of variance false discovery rate < 0.05, and their functional enrichment was analyzed using a network model, Gene Set Enrichment Analysis, and Gene Ontology annotation. Results We identified 9,890 proteins across all samples and 1,139 DEPs among the three entities. A substantial number of DEPs overlapped in CG/NU, distinct from UC. Interestingly, we found that a subset of DEP clusters (n = 53, 5%) was differentially expressed in NU but similarly between CG and UC. This “UC-like signature” was enriched for reactive oxygen species (ROS) and energy metabolism, growth and DNA repair, transport, motility, epithelial-mesenchymal transition, and cell survival. Using the top 10 shortlisted DEPs, including SOD2, PRKCD, CYCS, and HCLS1, we identified functional elements related to ROS metabolism, development, and transport using network analysis. The abundance of these four molecules in UC/CG than in NU was consistent with the oncologic functions in CG. Conclusions Using a proteomic approach, we identified a predominantly non-neoplastic landscape of CG, which was closer to NU than to UC. We also confirmed a small subset of common DEPs in UC and CG, suggesting that altered ROS metabolism might imply potential cancerous risks in CG.http://www.jpatholtm.org/upload/pdf/jptm-2022-10-24.pdfcystitisurinary bladder neoplasmscarcinomatransitional cellproteomicstandem mass spectrometry |
| spellingShingle | Jun Yong Kim Dohyun Han Hyeyoon Kim Minsun Jung Han Suk Ryu The proteomic landscape shows oncologic relevance in cystitis glandularis Journal of Pathology and Translational Medicine cystitis urinary bladder neoplasms carcinoma transitional cell proteomics tandem mass spectrometry |
| title | The proteomic landscape shows oncologic relevance in cystitis glandularis |
| title_full | The proteomic landscape shows oncologic relevance in cystitis glandularis |
| title_fullStr | The proteomic landscape shows oncologic relevance in cystitis glandularis |
| title_full_unstemmed | The proteomic landscape shows oncologic relevance in cystitis glandularis |
| title_short | The proteomic landscape shows oncologic relevance in cystitis glandularis |
| title_sort | proteomic landscape shows oncologic relevance in cystitis glandularis |
| topic | cystitis urinary bladder neoplasms carcinoma transitional cell proteomics tandem mass spectrometry |
| url | http://www.jpatholtm.org/upload/pdf/jptm-2022-10-24.pdf |
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