Development of high-throughput genotyping method of all 18 HR HPV based on the MALDI-TOF MS platform and compared with the Roche Cobas 4800 HPV assay using clinical specimens

Development of high-throughput genotyping method of all 18 HR HPV based on the MALDI-TOF MS platform and compared with the Roche Cobas 4800 HPV assay using clinical specimens

Abstract Background To develop a new 18 high-risk human papillomavirus (HR HPV) detection and genotyping assay, which is important to evaluate the risk degree of HR HPV for causing cancers. Methods All 18 HR HPV and β-globin relative DNA fragments were synthesized and cloned to a plasmid pUC57 to ob...

Full description

Bibliographic Details
Main Authors: Xushan Cai, Qinghua Guan, Yu Huan, Ziyu Liu, Jiehua Qi, Shichao Ge
Format: Article
Language:English
Published: BMC 2019-08-01
Series:BMC Cancer
Subjects:
Human papillomavirus
MALDI-TOF MS platform
Recombinant plasmid
The Roche Cobas 4800 HPV assay
Genotyping
Online Access:http://link.springer.com/article/10.1186/s12885-019-6036-z

Similar Items

Similar Items