Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection
The purpose of this paper is to evaluate the spreading of HIV-1 resistant to antiretroviral drugs among Tomsk Oblast population with newly diagnosed HIV-infection.Materials and methods. It was collected 122 clinical samples of peripheral blood of HIV-infected patients from Tomsk Oblast who did not...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2020-06-01
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| Series: | Журнал инфектологии |
| Subjects: | |
| Online Access: | https://journal.niidi.ru/jofin/article/view/1052 |
| _version_ | 1826557846364356608 |
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| author | D. P. Zyryanova E. M. Astakhova M. P. Gashnikova T. N. Ismailova E. F. Bocharov A. S. Chernov A. V. Totmenin M. N. Gashnikova |
| author_facet | D. P. Zyryanova E. M. Astakhova M. P. Gashnikova T. N. Ismailova E. F. Bocharov A. S. Chernov A. V. Totmenin M. N. Gashnikova |
| author_sort | D. P. Zyryanova |
| collection | DOAJ |
| description | The purpose of this paper is to evaluate the spreading of HIV-1 resistant to antiretroviral drugs among Tomsk Oblast population with newly diagnosed HIV-infection.Materials and methods. It was collected 122 clinical samples of peripheral blood of HIV-infected patients from Tomsk Oblast who did not take antiretroviral drugs. In HIV-1 isolated from clinical samples we studied nucleotide sequence of genome fragments encoding virus protease and reverse transcriptase. Complex analyses of epidemiologic data from patients and the presence in genome HIV-1 mutations associated with resistance development to protease inhibitors and virus reverse transcriptase were carried out.Results. Analysis of HIV-1 isolated from Tomsk Oblast naïve HIV-infected population made it possible to detect HIV-1 mutations associated with a decrease of virus sensitivity to antiretroviral drugs in 9,8% of cases. Among described mutations 50% were associated with resistance to virus protease inhibitors; 33,3% were resistance mutations to nonnuclease inhibitors of reverse transcriptase, and 16.7% were resistance mutations to nucleoside inhibitors of virus reverse transcriptase. Out of 9,8% of resistant viruses 7,3% of casesincluded mutations associated with the development of potentially low level of reduction of HIV-1 sensitivity to drugs. Main HIV-1 resistance mutations of high and average levels were registered only in 2,5% of genotyped HIV-1 isolated from people who inject drugs.Conclusion. Current study detected considerably low sampling rate of HIV-1 carrying mutations associated with resistance to antiretroviral drugs among Tomsk Oblast naïve HIV-infected population. It is believed to be caused by a relatively short period of extensive application of antiretroviral therapy in that territory. Analysis of epidemiologic data resulted in detection of factors negatively affecting prediction of further development of HIV-infection epidemic in the region including prevalence of risk behavior practice contributing to resistant HIV-1 transmission both among patients via heterosexual contacts and among people who inject drugs. |
| first_indexed | 2024-03-12T03:09:25Z |
| format | Article |
| id | doaj.art-8c38be71e17646bf8be5447a9064d3f2 |
| institution | Directory Open Access Journal |
| issn | 2072-6732 |
| language | Russian |
| last_indexed | 2025-03-14T08:35:05Z |
| publishDate | 2020-06-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj.art-8c38be71e17646bf8be5447a9064d3f22025-03-02T13:00:32ZrusJournal InfectologyЖурнал инфектологии2072-67322020-06-01122889610.22625/2072-6732-2020-12-2-88-96810Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infectionD. P. Zyryanova0E. M. Astakhova1M. P. Gashnikova2T. N. Ismailova3E. F. Bocharov4A. S. Chernov5A. V. Totmenin6M. N. Gashnikova7State Research Center of Virology and Biotechnology «Vector»State Research Center of Virology and Biotechnology «Vector»State Research Center of Virology and Biotechnology «Vector»Tomsk Regional Center for Prevention and Control of AIDSState Research Center of Virology and Biotechnology «Vector»Tomsk Regional Center for Prevention and Control of AIDSState Research Center of Virology and Biotechnology «Vector»State Research Center of Virology and Biotechnology «Vector»The purpose of this paper is to evaluate the spreading of HIV-1 resistant to antiretroviral drugs among Tomsk Oblast population with newly diagnosed HIV-infection.Materials and methods. It was collected 122 clinical samples of peripheral blood of HIV-infected patients from Tomsk Oblast who did not take antiretroviral drugs. In HIV-1 isolated from clinical samples we studied nucleotide sequence of genome fragments encoding virus protease and reverse transcriptase. Complex analyses of epidemiologic data from patients and the presence in genome HIV-1 mutations associated with resistance development to protease inhibitors and virus reverse transcriptase were carried out.Results. Analysis of HIV-1 isolated from Tomsk Oblast naïve HIV-infected population made it possible to detect HIV-1 mutations associated with a decrease of virus sensitivity to antiretroviral drugs in 9,8% of cases. Among described mutations 50% were associated with resistance to virus protease inhibitors; 33,3% were resistance mutations to nonnuclease inhibitors of reverse transcriptase, and 16.7% were resistance mutations to nucleoside inhibitors of virus reverse transcriptase. Out of 9,8% of resistant viruses 7,3% of casesincluded mutations associated with the development of potentially low level of reduction of HIV-1 sensitivity to drugs. Main HIV-1 resistance mutations of high and average levels were registered only in 2,5% of genotyped HIV-1 isolated from people who inject drugs.Conclusion. Current study detected considerably low sampling rate of HIV-1 carrying mutations associated with resistance to antiretroviral drugs among Tomsk Oblast naïve HIV-infected population. It is believed to be caused by a relatively short period of extensive application of antiretroviral therapy in that territory. Analysis of epidemiologic data resulted in detection of factors negatively affecting prediction of further development of HIV-infection epidemic in the region including prevalence of risk behavior practice contributing to resistant HIV-1 transmission both among patients via heterosexual contacts and among people who inject drugs.https://journal.niidi.ru/jofin/article/view/1052hiv-1resistant hiv-1 transmissiontomsk oblast |
| spellingShingle | D. P. Zyryanova E. M. Astakhova M. P. Gashnikova T. N. Ismailova E. F. Bocharov A. S. Chernov A. V. Totmenin M. N. Gashnikova Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection Журнал инфектологии hiv-1 resistant hiv-1 transmission tomsk oblast |
| title | Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection |
| title_full | Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection |
| title_fullStr | Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection |
| title_full_unstemmed | Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection |
| title_short | Detection of HIV-1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed HIV-infection |
| title_sort | detection of hiv 1 resistant to antiretroviral drugs among tomsk oblast population with newly diagnosed hiv infection |
| topic | hiv-1 resistant hiv-1 transmission tomsk oblast |
| url | https://journal.niidi.ru/jofin/article/view/1052 |
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