Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice
The cardiac voltage-gated sodium channel, Nav1.5, plays a central role in cardiac excitability and impulse propagation and associates with the dystrophin multiprotein complex (DMC) at the lateral membrane of cardiomyocytes. It was previously shown that Nav1.5 protein content and the sodium current (...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-03-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2013.00051/full |
| _version_ | 1811299042144026624 |
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| author | Jean-Sebastien eRougier Bruno eGavillet Hugues eAbriel |
| author_facet | Jean-Sebastien eRougier Bruno eGavillet Hugues eAbriel |
| author_sort | Jean-Sebastien eRougier |
| collection | DOAJ |
| description | The cardiac voltage-gated sodium channel, Nav1.5, plays a central role in cardiac excitability and impulse propagation and associates with the dystrophin multiprotein complex (DMC) at the lateral membrane of cardiomyocytes. It was previously shown that Nav1.5 protein content and the sodium current (INa) were both decreased in cardiomyocytes of dystrophin-deficient mdx5cv mice. In this study, wild-type (WT) and mdx5cv mice were treated for 7 days with the proteasome inhibitor MG132 (10 µg/Kg/24 h) using implanted osmotic mini pumps. MG132 rescued both the total amount of Nav1.5 protein and INa but, unlike in previous studies, de novo expression of dystrophin was not observed in skeletal or cardiac muscle. This study suggests that the reduced expression of Nav1.5 in dystrophin-deficient cells is dependent on proteasomal degradation. |
| first_indexed | 2024-04-13T06:30:13Z |
| format | Article |
| id | doaj.art-8c3afb58444d4855823ab142810b2494 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T06:30:13Z |
| publishDate | 2013-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-8c3afb58444d4855823ab142810b24942022-12-22T02:58:13ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2013-03-01410.3389/fphys.2013.0005142786Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv miceJean-Sebastien eRougier0Bruno eGavillet1Hugues eAbriel2University of Bern: UNIBEUniversity of Bern: UNIBEUniversity of Bern: UNIBEThe cardiac voltage-gated sodium channel, Nav1.5, plays a central role in cardiac excitability and impulse propagation and associates with the dystrophin multiprotein complex (DMC) at the lateral membrane of cardiomyocytes. It was previously shown that Nav1.5 protein content and the sodium current (INa) were both decreased in cardiomyocytes of dystrophin-deficient mdx5cv mice. In this study, wild-type (WT) and mdx5cv mice were treated for 7 days with the proteasome inhibitor MG132 (10 µg/Kg/24 h) using implanted osmotic mini pumps. MG132 rescued both the total amount of Nav1.5 protein and INa but, unlike in previous studies, de novo expression of dystrophin was not observed in skeletal or cardiac muscle. This study suggests that the reduced expression of Nav1.5 in dystrophin-deficient cells is dependent on proteasomal degradation.http://journal.frontiersin.org/Journal/10.3389/fphys.2013.00051/fullDystrophinElectrophysiologySodium ChannelsinhibitorsProteasome |
| spellingShingle | Jean-Sebastien eRougier Bruno eGavillet Hugues eAbriel Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice Frontiers in Physiology Dystrophin Electrophysiology Sodium Channels inhibitors Proteasome |
| title | Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice |
| title_full | Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice |
| title_fullStr | Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice |
| title_full_unstemmed | Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice |
| title_short | Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx5cv mice |
| title_sort | proteasome inhibitor mg132 rescues nav1 5 protein content and the cardiac sodium current in dystrophin deficient mdx5cv mice |
| topic | Dystrophin Electrophysiology Sodium Channels inhibitors Proteasome |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2013.00051/full |
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