Effects of Focused Vibrations on Human Satellite Cells
Skeletal muscle consists of long plurinucleate and contractile structures, able to regenerate and repair tissue damage by their resident stem cells: satellite cells (SCs). Reduced skeletal muscle regeneration and progressive atrophy are typical features of sarcopenia, which has important health care...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/6026 |
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| author | Silvia Sancilio Sara Nobilio Antonio Giulio Ruggiero Ester Sara Di Filippo Gianmarco Stati Stefania Fulle Rosa Grazia Bellomo Raoul Saggini Roberta Di Pietro |
| author_facet | Silvia Sancilio Sara Nobilio Antonio Giulio Ruggiero Ester Sara Di Filippo Gianmarco Stati Stefania Fulle Rosa Grazia Bellomo Raoul Saggini Roberta Di Pietro |
| author_sort | Silvia Sancilio |
| collection | DOAJ |
| description | Skeletal muscle consists of long plurinucleate and contractile structures, able to regenerate and repair tissue damage by their resident stem cells: satellite cells (SCs). Reduced skeletal muscle regeneration and progressive atrophy are typical features of sarcopenia, which has important health care implications for humans. Sarcopenia treatment is usually based on physical exercise and nutritional plans, possibly associated with rehabilitation programs, such as vibratory stimulation. Vibrations stimulate muscles and can increase postural stability, balance, and walking in aged and sarcopenic patients. However, the possible direct effect of vibration on SCs is still unclear. Here, we show the effects of focused vibrations administered at increasing time intervals on SCs, isolated from young and aged subjects and cultured in vitro. After stimulations, we found in both young and aged subjects a reduced percentage of apoptotic cells, increased cell size and percentage of aligned cells, mitotic events, and activated cells. We also found an increased number of cells only in young samples. Our results highlight for the first time the presence of direct effects of mechanical vibrations on human SCs. These effects seem to be age-dependent, consisting of a proliferative response of cells derived from young subjects vs. a differentiative response of cells from aged subjects. |
| first_indexed | 2024-03-10T01:16:14Z |
| format | Article |
| id | doaj.art-8c3ce3f9120840cda8d81c269933bfd9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:16:14Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8c3ce3f9120840cda8d81c269933bfd92023-11-23T14:08:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311602610.3390/ijms23116026Effects of Focused Vibrations on Human Satellite CellsSilvia Sancilio0Sara Nobilio1Antonio Giulio Ruggiero2Ester Sara Di Filippo3Gianmarco Stati4Stefania Fulle5Rosa Grazia Bellomo6Raoul Saggini7Roberta Di Pietro8Department of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Neuroscience Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Neuroscience Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Biomolecular Sciences, “Carlo Bo” University, Via Aurelio Saffi 2, 61029 Urbino, ItalyDepartment of Medical and Oral Sciences and Biotechnologies, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalySkeletal muscle consists of long plurinucleate and contractile structures, able to regenerate and repair tissue damage by their resident stem cells: satellite cells (SCs). Reduced skeletal muscle regeneration and progressive atrophy are typical features of sarcopenia, which has important health care implications for humans. Sarcopenia treatment is usually based on physical exercise and nutritional plans, possibly associated with rehabilitation programs, such as vibratory stimulation. Vibrations stimulate muscles and can increase postural stability, balance, and walking in aged and sarcopenic patients. However, the possible direct effect of vibration on SCs is still unclear. Here, we show the effects of focused vibrations administered at increasing time intervals on SCs, isolated from young and aged subjects and cultured in vitro. After stimulations, we found in both young and aged subjects a reduced percentage of apoptotic cells, increased cell size and percentage of aligned cells, mitotic events, and activated cells. We also found an increased number of cells only in young samples. Our results highlight for the first time the presence of direct effects of mechanical vibrations on human SCs. These effects seem to be age-dependent, consisting of a proliferative response of cells derived from young subjects vs. a differentiative response of cells from aged subjects.https://www.mdpi.com/1422-0067/23/11/6026focused mechanoacoustic vibrationhuman satellite cellsagingsarcopeniaatrophyrehabilitation |
| spellingShingle | Silvia Sancilio Sara Nobilio Antonio Giulio Ruggiero Ester Sara Di Filippo Gianmarco Stati Stefania Fulle Rosa Grazia Bellomo Raoul Saggini Roberta Di Pietro Effects of Focused Vibrations on Human Satellite Cells International Journal of Molecular Sciences focused mechanoacoustic vibration human satellite cells aging sarcopenia atrophy rehabilitation |
| title | Effects of Focused Vibrations on Human Satellite Cells |
| title_full | Effects of Focused Vibrations on Human Satellite Cells |
| title_fullStr | Effects of Focused Vibrations on Human Satellite Cells |
| title_full_unstemmed | Effects of Focused Vibrations on Human Satellite Cells |
| title_short | Effects of Focused Vibrations on Human Satellite Cells |
| title_sort | effects of focused vibrations on human satellite cells |
| topic | focused mechanoacoustic vibration human satellite cells aging sarcopenia atrophy rehabilitation |
| url | https://www.mdpi.com/1422-0067/23/11/6026 |
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