Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience
New microRNA-based serum biomarkers (miRNA-367-3p, -371a-3p, -372-3p, and -373-3p) have shown great potential for the detection of testicular germ cell tumors (TGCTs), but few studies have investigated the clinical utility and performance of these tests in treatment monitoring. In this study, circul...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/3/759 |
| _version_ | 1797728559864741888 |
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| author | Nina Mørup Ewa Rajpert-De Meyts Anders Juul Gedske Daugaard Kristian Almstrup |
| author_facet | Nina Mørup Ewa Rajpert-De Meyts Anders Juul Gedske Daugaard Kristian Almstrup |
| author_sort | Nina Mørup |
| collection | DOAJ |
| description | New microRNA-based serum biomarkers (miRNA-367-3p, -371a-3p, -372-3p, and -373-3p) have shown great potential for the detection of testicular germ cell tumors (TGCTs), but few studies have investigated the clinical utility and performance of these tests in treatment monitoring. In this study, circulating miRNA levels were measured, together with serum tumor markers alpha-fetoprotein (AFP), β-subunit of human chorionic gonadotropin (β-HCG) and lactate dehydrogenase (LDH) in 406 consecutive blood samples obtained during the treatment and follow-up of 52 TGCT patients at the Copenhagen University Hospital. After testing three different methods of RNA isolation from peripheral blood and PCR quantification in a subset of samples (n = 15), the best performing setup of targeted isolation of miRNAs inside and outside exosomes was selected to analyze all samples. At primary diagnosis, the miRNAs significantly outperformed the serum tumor markers, with a sensitivity and specificity of 78% and 100% (based on 40 patients), respectively. The picture was not as clear when patient trajectories were investigated, with both positive and negative signals for miRNAs and serum tumor markers. To establish whether measuring miRNAs adds value beyond the primary diagnosis, large prospective clinical trials comparing miRNAs and classical tumor markers during the treatment and follow-up of TGCT patients are needed. |
| first_indexed | 2024-03-12T11:15:49Z |
| format | Article |
| id | doaj.art-8c3f1ded240f4e88a3398c0a8a658b46 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T11:15:49Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-8c3f1ded240f4e88a3398c0a8a658b462023-09-02T02:02:00ZengMDPI AGCancers2072-66942020-03-0112375910.3390/cancers12030759cancers12030759Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen ExperienceNina Mørup0Ewa Rajpert-De Meyts1Anders Juul2Gedske Daugaard3Kristian Almstrup4Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen 2100, DenmarkDepartment of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen 2100, DenmarkDepartment of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen 2100, DenmarkDepartment of Oncology, Rigshospitalet, University of Copenhagen, Copenhagen 2100, DenmarkDepartment of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen 2100, DenmarkNew microRNA-based serum biomarkers (miRNA-367-3p, -371a-3p, -372-3p, and -373-3p) have shown great potential for the detection of testicular germ cell tumors (TGCTs), but few studies have investigated the clinical utility and performance of these tests in treatment monitoring. In this study, circulating miRNA levels were measured, together with serum tumor markers alpha-fetoprotein (AFP), β-subunit of human chorionic gonadotropin (β-HCG) and lactate dehydrogenase (LDH) in 406 consecutive blood samples obtained during the treatment and follow-up of 52 TGCT patients at the Copenhagen University Hospital. After testing three different methods of RNA isolation from peripheral blood and PCR quantification in a subset of samples (n = 15), the best performing setup of targeted isolation of miRNAs inside and outside exosomes was selected to analyze all samples. At primary diagnosis, the miRNAs significantly outperformed the serum tumor markers, with a sensitivity and specificity of 78% and 100% (based on 40 patients), respectively. The picture was not as clear when patient trajectories were investigated, with both positive and negative signals for miRNAs and serum tumor markers. To establish whether measuring miRNAs adds value beyond the primary diagnosis, large prospective clinical trials comparing miRNAs and classical tumor markers during the treatment and follow-up of TGCT patients are needed.https://www.mdpi.com/2072-6694/12/3/759mirnamir-371aafphcgtesticular germ cell cancerchemotherapyfollow-uptgct |
| spellingShingle | Nina Mørup Ewa Rajpert-De Meyts Anders Juul Gedske Daugaard Kristian Almstrup Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience Cancers mirna mir-371a afp hcg testicular germ cell cancer chemotherapy follow-up tgct |
| title | Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience |
| title_full | Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience |
| title_fullStr | Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience |
| title_full_unstemmed | Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience |
| title_short | Evaluation of Circulating miRNA Biomarkers of Testicular Germ Cell Tumors during Therapy and Follow-up―A Copenhagen Experience |
| title_sort | evaluation of circulating mirna biomarkers of testicular germ cell tumors during therapy and follow up a copenhagen experience |
| topic | mirna mir-371a afp hcg testicular germ cell cancer chemotherapy follow-up tgct |
| url | https://www.mdpi.com/2072-6694/12/3/759 |
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