In vitro Scolicidal Effect of Lepidium sativum Essential Oil
Background & objectives: One of the most important zoonotic parasitic diseases, hydatidosis, is caused by the larval stage of Echinococcous granulosus. Investigations have shown that plants secondary metabolites, such as essential oils have anti parasitic properties. Based on previous reports on...
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| Format: | Article |
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Ardabil University of Medical Sciences
2016-01-01
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| Series: | Journal of Ardabil University of Medical Sciences |
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| Online Access: | http://jarums.arums.ac.ir/browse.php?a_code=A-10-27-656&slc_lang=en&sid=1 |
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| author | Somaye Bahrami Mohammad hossein Razi Jalali Zahra Ramezani Mehdi Pourmehdi Boroujeni Ferial Toeimepour |
| author_facet | Somaye Bahrami Mohammad hossein Razi Jalali Zahra Ramezani Mehdi Pourmehdi Boroujeni Ferial Toeimepour |
| author_sort | Somaye Bahrami |
| collection | DOAJ |
| description | Background & objectives: One of the most important zoonotic parasitic diseases, hydatidosis, is caused by the larval stage of Echinococcous granulosus. Investigations have shown that plants secondary metabolites, such as essential oils have anti parasitic properties. Based on previous reports on antiparasitic properties of Lepidium sativum, in this study we investigated the scolicidal effects of the essential oil (EO) extracted from this plant.
Methods: Lepidium EO was obtained by hydrodistillation method. Gas chromatography-mass spectrometry (GC-MS) was employed to determine the chemical composition of the EO. Protoscolices were exposed to various concentrations of EO (1, 3, 5, 10 and 15 mg/ml) for 10, 20, 30 and 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining.
Results: A total of 19 compounds representing 95.5% of the total oil, were identified. α-Thujene (88.86%), Myrcene (2.9%) and P-cymene (1.67%) were found to be the major EO constituents. Based on the results, protoscolices mortality rates at 1, 3 and 5 mg/ml of EO didn’t have a significant relationship with the control group. While, the difference in mortality rate at a concentration of 10 mg/ml of EO in 30 and 60 min was significant. Also, the concentration of 15 mg/ml of EO at all times of incubation had significantly higher protoscolicidal effect. In the present study there was a significant relation between the amount of protoscolicidal activity of different EO concentrations and different incubation times. In other words mortality rates enhanced with increasing concentrations and incubation times.
Conclusion: The results of present study revealed that the EO of Lepidium is rich in α-Thujene and has a high scolicidal power. This plant may be used as a natural scolicidal agent |
| first_indexed | 2024-12-19T08:28:47Z |
| format | Article |
| id | doaj.art-8c3f8135e9cb471993808816c137251e |
| institution | Directory Open Access Journal |
| issn | 2228-7280 2228-7299 |
| language | fas |
| last_indexed | 2024-12-19T08:28:47Z |
| publishDate | 2016-01-01 |
| publisher | Ardabil University of Medical Sciences |
| record_format | Article |
| series | Journal of Ardabil University of Medical Sciences |
| spelling | doaj.art-8c3f8135e9cb471993808816c137251e2022-12-21T20:29:15ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992016-01-01154395403In vitro Scolicidal Effect of Lepidium sativum Essential OilSomaye Bahrami0Mohammad hossein Razi Jalali1Zahra Ramezani2Mehdi Pourmehdi Boroujeni3Ferial Toeimepour4 Background & objectives: One of the most important zoonotic parasitic diseases, hydatidosis, is caused by the larval stage of Echinococcous granulosus. Investigations have shown that plants secondary metabolites, such as essential oils have anti parasitic properties. Based on previous reports on antiparasitic properties of Lepidium sativum, in this study we investigated the scolicidal effects of the essential oil (EO) extracted from this plant. Methods: Lepidium EO was obtained by hydrodistillation method. Gas chromatography-mass spectrometry (GC-MS) was employed to determine the chemical composition of the EO. Protoscolices were exposed to various concentrations of EO (1, 3, 5, 10 and 15 mg/ml) for 10, 20, 30 and 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Results: A total of 19 compounds representing 95.5% of the total oil, were identified. α-Thujene (88.86%), Myrcene (2.9%) and P-cymene (1.67%) were found to be the major EO constituents. Based on the results, protoscolices mortality rates at 1, 3 and 5 mg/ml of EO didn’t have a significant relationship with the control group. While, the difference in mortality rate at a concentration of 10 mg/ml of EO in 30 and 60 min was significant. Also, the concentration of 15 mg/ml of EO at all times of incubation had significantly higher protoscolicidal effect. In the present study there was a significant relation between the amount of protoscolicidal activity of different EO concentrations and different incubation times. In other words mortality rates enhanced with increasing concentrations and incubation times. Conclusion: The results of present study revealed that the EO of Lepidium is rich in α-Thujene and has a high scolicidal power. This plant may be used as a natural scolicidal agenthttp://jarums.arums.ac.ir/browse.php?a_code=A-10-27-656&slc_lang=en&sid=1Lepidium sativum Essential Oil Protoscolex Hydatid Cyst In vitro |
| spellingShingle | Somaye Bahrami Mohammad hossein Razi Jalali Zahra Ramezani Mehdi Pourmehdi Boroujeni Ferial Toeimepour In vitro Scolicidal Effect of Lepidium sativum Essential Oil Journal of Ardabil University of Medical Sciences Lepidium sativum Essential Oil Protoscolex Hydatid Cyst In vitro |
| title | In vitro Scolicidal Effect of Lepidium sativum Essential Oil |
| title_full | In vitro Scolicidal Effect of Lepidium sativum Essential Oil |
| title_fullStr | In vitro Scolicidal Effect of Lepidium sativum Essential Oil |
| title_full_unstemmed | In vitro Scolicidal Effect of Lepidium sativum Essential Oil |
| title_short | In vitro Scolicidal Effect of Lepidium sativum Essential Oil |
| title_sort | in vitro scolicidal effect of lepidium sativum essential oil |
| topic | Lepidium sativum Essential Oil Protoscolex Hydatid Cyst In vitro |
| url | http://jarums.arums.ac.ir/browse.php?a_code=A-10-27-656&slc_lang=en&sid=1 |
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