Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures
Abstract Hazelnut is one of the most important nut plants recently suggested as a sustainable source for paclitaxel. In the present study, the effect of the concentration and combination of PGRs, different basal medium and ultrasonic waves on callus induction and growth, physiological characteristic...
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| Format: | Article |
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SpringerOpen
2022-08-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-022-01449-z |
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| author | Roghayyeh Hazrati Nasser Zare Rasool Asghari-Zakaria Parisa Sheikhzadeh Mohammad Johari-Ahar |
| author_facet | Roghayyeh Hazrati Nasser Zare Rasool Asghari-Zakaria Parisa Sheikhzadeh Mohammad Johari-Ahar |
| author_sort | Roghayyeh Hazrati |
| collection | DOAJ |
| description | Abstract Hazelnut is one of the most important nut plants recently suggested as a sustainable source for paclitaxel. In the present study, the effect of the concentration and combination of PGRs, different basal medium and ultrasonic waves on callus induction and growth, physiological characteristics, and taxol and baccatin III production in hazelnut callus cultures were investigated. The results indicated that combining 2,4-D (2 mg/L) and Kin (0.2 mg/L) with the sonication of explants for 1 min provides an optimized condition for callus induction and growth. Hazelnut explants exhibited different callus production and biochemical and metabolic characteristics depending on the basal medium type, ultrasound treatment, and inclusion of ascorbic acid in the medium. So that, the highest percentage of callogenesis (100%) observed in ½ MS + 1 min US, ½ MS + 150 mg/L AA, B5 + 1 min US and B5 + 150 mg/L AA, and also ½ MS salt + Nitsch vitamins + 150 mg/L AA. Furthermore, the highest callus growth (7.86 g FW) was obtained from ½ MS + 1 min US. The highest amount of baccatin III production (147.98 and 147.85 mg/L) was obtained from the WPM and MS basal media; the highest taxol production (44.89 mg/L) was observed in the WPM basal medium. The cultures in the MS, WPM, and MS salts + Nitsch vitamins media, had the highest H2O2 and MDA content, antioxidant enzymes activity, and phenolic compounds. In conclusion, culture media nutrient composition and concentration not only affect the cell growth and physiological status of the cultures but also improve secondary metabolites production and accumulation. |
| first_indexed | 2024-12-10T18:51:53Z |
| format | Article |
| id | doaj.art-8c4110bddb31466aaaa23bbe77a7029c |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-10T18:51:53Z |
| publishDate | 2022-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-8c4110bddb31466aaaa23bbe77a7029c2022-12-22T01:37:17ZengSpringerOpenAMB Express2191-08552022-08-0112111610.1186/s13568-022-01449-zFactors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus culturesRoghayyeh Hazrati0Nasser Zare1Rasool Asghari-Zakaria2Parisa Sheikhzadeh3Mohammad Johari-Ahar4Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliPlant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliPlant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliPlant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliDepartment of Medicinal Chemistry, School of Pharmacy, Ardabil University of Medical SciencesAbstract Hazelnut is one of the most important nut plants recently suggested as a sustainable source for paclitaxel. In the present study, the effect of the concentration and combination of PGRs, different basal medium and ultrasonic waves on callus induction and growth, physiological characteristics, and taxol and baccatin III production in hazelnut callus cultures were investigated. The results indicated that combining 2,4-D (2 mg/L) and Kin (0.2 mg/L) with the sonication of explants for 1 min provides an optimized condition for callus induction and growth. Hazelnut explants exhibited different callus production and biochemical and metabolic characteristics depending on the basal medium type, ultrasound treatment, and inclusion of ascorbic acid in the medium. So that, the highest percentage of callogenesis (100%) observed in ½ MS + 1 min US, ½ MS + 150 mg/L AA, B5 + 1 min US and B5 + 150 mg/L AA, and also ½ MS salt + Nitsch vitamins + 150 mg/L AA. Furthermore, the highest callus growth (7.86 g FW) was obtained from ½ MS + 1 min US. The highest amount of baccatin III production (147.98 and 147.85 mg/L) was obtained from the WPM and MS basal media; the highest taxol production (44.89 mg/L) was observed in the WPM basal medium. The cultures in the MS, WPM, and MS salts + Nitsch vitamins media, had the highest H2O2 and MDA content, antioxidant enzymes activity, and phenolic compounds. In conclusion, culture media nutrient composition and concentration not only affect the cell growth and physiological status of the cultures but also improve secondary metabolites production and accumulation.https://doi.org/10.1186/s13568-022-01449-zAntioxidant enzymesBaccatin IIICallus cultureSecondary metabolitesSonicationTaxol |
| spellingShingle | Roghayyeh Hazrati Nasser Zare Rasool Asghari-Zakaria Parisa Sheikhzadeh Mohammad Johari-Ahar Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures AMB Express Antioxidant enzymes Baccatin III Callus culture Secondary metabolites Sonication Taxol |
| title | Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures |
| title_full | Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures |
| title_fullStr | Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures |
| title_full_unstemmed | Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures |
| title_short | Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures |
| title_sort | factors affecting the growth antioxidant potential and secondary metabolites production in hazel callus cultures |
| topic | Antioxidant enzymes Baccatin III Callus culture Secondary metabolites Sonication Taxol |
| url | https://doi.org/10.1186/s13568-022-01449-z |
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