N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer
BackgroundLncRNA dysregulation and the tumor microenvironment (TME) have been shown to play a vital role in the progression and prognosis of colon cancer (CC). We aim to reveal the potential molecular mechanism from the perspective of lncRNA in the TME and provide the candidate biomarkers for CC pro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.697949/full |
| _version_ | 1819070481449353216 |
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| author | Hongliang Zhang Hongliang Zhang Hongliang Zhang Lei Zhao Songyan Li Jing Wang Cong Feng Tanshi Li Xiaohui Du |
| author_facet | Hongliang Zhang Hongliang Zhang Hongliang Zhang Lei Zhao Songyan Li Jing Wang Cong Feng Tanshi Li Xiaohui Du |
| author_sort | Hongliang Zhang |
| collection | DOAJ |
| description | BackgroundLncRNA dysregulation and the tumor microenvironment (TME) have been shown to play a vital role in the progression and prognosis of colon cancer (CC). We aim to reveal the potential molecular mechanism from the perspective of lncRNA in the TME and provide the candidate biomarkers for CC prognosis.MethodsESTIMATE analysis was used to divide the CC patients into high and low immune or stromal score groups. The expression array of lncRNA was re-annotated by Seqmap. Microenvironment-associated lncRNAs were filtered through differential analysis. The m6A-associated lncRNAs were screened by Pearson correlation analysis. Lasso Cox regression analyses were performed to construct the m6A- and tumor microenvironment-related lncRNA prognostic model (m6A-TME-LM). Survival analysis was used to assess the prognostic efficacy of candidate lncRNAs. Enrichment analyses annotated the candidate genes’ functions.ResultsWe obtained 25 common differentially expressed lncRNAs (DELs) associated with immune microenvironment and m6A-related genes for subsequent lasso analysis. Four out of these DELs were selected for the m6A-TME-LM. All the four lncRNAs were related to overall survival, and a test set testified the result. Further stratification analysis of the m6A-TME-LM retained its ability to predict OS for male and chemotherapy adjuvant patients and performed an excellent prognostic efficacy in the TNM stage III and IV subgroups. Network analysis also found the four lncRNAs mediated co-expression network was associated with tumor development.ConclusionWe constructed the m6A-TME-LM, which could provide a better prognostic prediction of CC. |
| first_indexed | 2024-12-21T17:06:37Z |
| format | Article |
| id | doaj.art-8c41996d03f44520931979b52a94e62e |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-21T17:06:37Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-8c41996d03f44520931979b52a94e62e2022-12-21T18:56:31ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-06-011110.3389/fonc.2021.697949697949N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon CancerHongliang Zhang0Hongliang Zhang1Hongliang Zhang2Lei Zhao3Songyan Li4Jing Wang5Cong Feng6Tanshi Li7Xiaohui Du8Medical School of Chinese People's Liberation Army (PLA), Beijing, ChinaDepartment of Emergency, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaThe 65651 Army of the Chinese PLA, Jinzhou, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Peking University People’s Hospital, Beijing, ChinaDepartment of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Emergency, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Emergency, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Emergency, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaBackgroundLncRNA dysregulation and the tumor microenvironment (TME) have been shown to play a vital role in the progression and prognosis of colon cancer (CC). We aim to reveal the potential molecular mechanism from the perspective of lncRNA in the TME and provide the candidate biomarkers for CC prognosis.MethodsESTIMATE analysis was used to divide the CC patients into high and low immune or stromal score groups. The expression array of lncRNA was re-annotated by Seqmap. Microenvironment-associated lncRNAs were filtered through differential analysis. The m6A-associated lncRNAs were screened by Pearson correlation analysis. Lasso Cox regression analyses were performed to construct the m6A- and tumor microenvironment-related lncRNA prognostic model (m6A-TME-LM). Survival analysis was used to assess the prognostic efficacy of candidate lncRNAs. Enrichment analyses annotated the candidate genes’ functions.ResultsWe obtained 25 common differentially expressed lncRNAs (DELs) associated with immune microenvironment and m6A-related genes for subsequent lasso analysis. Four out of these DELs were selected for the m6A-TME-LM. All the four lncRNAs were related to overall survival, and a test set testified the result. Further stratification analysis of the m6A-TME-LM retained its ability to predict OS for male and chemotherapy adjuvant patients and performed an excellent prognostic efficacy in the TNM stage III and IV subgroups. Network analysis also found the four lncRNAs mediated co-expression network was associated with tumor development.ConclusionWe constructed the m6A-TME-LM, which could provide a better prognostic prediction of CC.https://www.frontiersin.org/articles/10.3389/fonc.2021.697949/fullcolon cancertumor microenvironmentN6-methylandenosine (m6A)lncRNA - long noncoding RNAprognostic biomarker |
| spellingShingle | Hongliang Zhang Hongliang Zhang Hongliang Zhang Lei Zhao Songyan Li Jing Wang Cong Feng Tanshi Li Xiaohui Du N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer Frontiers in Oncology colon cancer tumor microenvironment N6-methylandenosine (m6A) lncRNA - long noncoding RNA prognostic biomarker |
| title | N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer |
| title_full | N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer |
| title_fullStr | N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer |
| title_full_unstemmed | N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer |
| title_short | N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer |
| title_sort | n6 methylandenosine related lncrnas in tumor microenvironment are potential prognostic biomarkers in colon cancer |
| topic | colon cancer tumor microenvironment N6-methylandenosine (m6A) lncRNA - long noncoding RNA prognostic biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.697949/full |
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