Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i>
The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/1/56 |
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| author | Lorena Ruiz-Montoya Mayren Sánchez-Rosario Emiliano López-Gómez Maricela Garcia-Bautista Anahí Canedo-Texón David Haymer Pablo Liedo |
| author_facet | Lorena Ruiz-Montoya Mayren Sánchez-Rosario Emiliano López-Gómez Maricela Garcia-Bautista Anahí Canedo-Texón David Haymer Pablo Liedo |
| author_sort | Lorena Ruiz-Montoya |
| collection | DOAJ |
| description | The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, <i>Anastrepha ludens,</i> and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains. |
| first_indexed | 2024-03-08T10:46:24Z |
| format | Article |
| id | doaj.art-8c439d561a284b97915ec3f9c4a3c8cd |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-08T10:46:24Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-8c439d561a284b97915ec3f9c4a3c8cd2024-01-26T17:04:16ZengMDPI AGInsects2075-44502024-01-011515610.3390/insects15010056Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i>Lorena Ruiz-Montoya0Mayren Sánchez-Rosario1Emiliano López-Gómez2Maricela Garcia-Bautista3Anahí Canedo-Texón4David Haymer5Pablo Liedo6El Colegio de la Frontera Sur (ECOSUR), Carretera Panamericana y Periférico Sur, Barrio María Auxiliadora, San Cristóbal de las Casas 29290, Chiapas, MexicoEl Colegio de la Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto, Tapachula 30700, Chiapas, MexicoInstituto de Biociencias, Universidad Autónoma de Chiapas, Boulevard Príncipe Akishino Sin Número Colonia Solidaridad 2000, Tapachula 30798, Chiapas, MexicoEl Colegio de la Frontera Sur (ECOSUR), Carretera Panamericana y Periférico Sur, Barrio María Auxiliadora, San Cristóbal de las Casas 29290, Chiapas, MexicoEl Colegio de la Frontera Sur (ECOSUR), Carretera Panamericana y Periférico Sur, Barrio María Auxiliadora, San Cristóbal de las Casas 29290, Chiapas, MexicoDepartment of Cell and Molecular Biology, University of Hawaii, 1960 East-West Rd, Biomed T511, Honolulu, HI 96822, USAEl Colegio de la Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto, Tapachula 30700, Chiapas, MexicoThe application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, <i>Anastrepha ludens,</i> and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains.https://www.mdpi.com/2075-4450/15/1/56Tephritidaegenetic differentiationinsect pestmass-rearing adaptationmicrosatellites markerssterile insect technique |
| spellingShingle | Lorena Ruiz-Montoya Mayren Sánchez-Rosario Emiliano López-Gómez Maricela Garcia-Bautista Anahí Canedo-Texón David Haymer Pablo Liedo Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i> Insects Tephritidae genetic differentiation insect pest mass-rearing adaptation microsatellites markers sterile insect technique |
| title | Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i> |
| title_full | Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i> |
| title_fullStr | Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i> |
| title_full_unstemmed | Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i> |
| title_short | Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, <i>Anastrepha ludens (Diptera: Tephritidae)</i> |
| title_sort | mass rearing conditions do not always reduce genetic diversity the case of the mexican fruit fly i anastrepha ludens diptera tephritidae i |
| topic | Tephritidae genetic differentiation insect pest mass-rearing adaptation microsatellites markers sterile insect technique |
| url | https://www.mdpi.com/2075-4450/15/1/56 |
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