<i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3
The urgent need to combat antibiotic resistance and develop novel antimicrobial therapies has triggered studies on novel metal-based formulations. <i>N</i>-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer and/or antimicrobial agent...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/348 |
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| author | Magdalena Piatek Cillian O’Beirne Zoe Beato Matthias Tacke Kevin Kavanagh |
| author_facet | Magdalena Piatek Cillian O’Beirne Zoe Beato Matthias Tacke Kevin Kavanagh |
| author_sort | Magdalena Piatek |
| collection | DOAJ |
| description | The urgent need to combat antibiotic resistance and develop novel antimicrobial therapies has triggered studies on novel metal-based formulations. <i>N</i>-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer and/or antimicrobial agents, with ongoing efforts being made to enhance the lipophilicity and drug stability. The lead silver(I) acetate complex, 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*) (SBC3), has previously demonstrated promising growth and biofilm-inhibiting properties. In this work, the responses of two structurally different bacteria to SBC3 using label-free quantitative proteomics were characterised. Multidrug-resistant <i>Pseudomonas aeruginosa</i> (Gram-negative) and <i>Staphylococcus aureus</i> (Gram-positive) are associated with cystic fibrosis lung colonisation and chronic wound infections, respectively. SBC3 increased the abundance of alginate biosynthesis, the secretion system and drug detoxification proteins in <i>P. aeruginosa</i>, whilst a variety of pathways, including anaerobic respiration, twitching motility and ABC transport, were decreased in abundance. This contrasted the affected pathways in <i>S. aureus</i>, where increased DNA replication/repair and cell redox homeostasis and decreased protein synthesis, lipoylation and glucose metabolism were observed. Increased abundance of cell wall/membrane proteins was indicative of the structural damage induced by SBC3 in both bacteria. These findings show the potential broad applications of SBC3 in treating Gram-positive and Gram-negative bacteria. |
| first_indexed | 2024-03-11T09:15:09Z |
| format | Article |
| id | doaj.art-8c45e4773dac4a6aaa7557794e09e788 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:15:09Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-8c45e4773dac4a6aaa7557794e09e7882023-11-16T18:43:45ZengMDPI AGAntibiotics2079-63822023-02-0112234810.3390/antibiotics12020348<i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3Magdalena Piatek0Cillian O’Beirne1Zoe Beato2Matthias Tacke3Kevin Kavanagh4SSPC Pharma Research Centre, Department of Biology, Maynooth University, W23 F2K8 Maynooth, Co. Kildare, IrelandSchool of Chemistry, University College Dublin, D04 V1W8 Belfield, Dublin 4, IrelandSchool of Chemistry, University College Dublin, D04 V1W8 Belfield, Dublin 4, IrelandSchool of Chemistry, University College Dublin, D04 V1W8 Belfield, Dublin 4, IrelandSSPC Pharma Research Centre, Department of Biology, Maynooth University, W23 F2K8 Maynooth, Co. Kildare, IrelandThe urgent need to combat antibiotic resistance and develop novel antimicrobial therapies has triggered studies on novel metal-based formulations. <i>N</i>-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer and/or antimicrobial agents, with ongoing efforts being made to enhance the lipophilicity and drug stability. The lead silver(I) acetate complex, 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*) (SBC3), has previously demonstrated promising growth and biofilm-inhibiting properties. In this work, the responses of two structurally different bacteria to SBC3 using label-free quantitative proteomics were characterised. Multidrug-resistant <i>Pseudomonas aeruginosa</i> (Gram-negative) and <i>Staphylococcus aureus</i> (Gram-positive) are associated with cystic fibrosis lung colonisation and chronic wound infections, respectively. SBC3 increased the abundance of alginate biosynthesis, the secretion system and drug detoxification proteins in <i>P. aeruginosa</i>, whilst a variety of pathways, including anaerobic respiration, twitching motility and ABC transport, were decreased in abundance. This contrasted the affected pathways in <i>S. aureus</i>, where increased DNA replication/repair and cell redox homeostasis and decreased protein synthesis, lipoylation and glucose metabolism were observed. Increased abundance of cell wall/membrane proteins was indicative of the structural damage induced by SBC3 in both bacteria. These findings show the potential broad applications of SBC3 in treating Gram-positive and Gram-negative bacteria.https://www.mdpi.com/2079-6382/12/2/348antimicrobialproteomesilver<i>Pseudomonas</i><i>Staphylococcus</i> |
| spellingShingle | Magdalena Piatek Cillian O’Beirne Zoe Beato Matthias Tacke Kevin Kavanagh <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3 Antibiotics antimicrobial proteome silver <i>Pseudomonas</i> <i>Staphylococcus</i> |
| title | <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3 |
| title_full | <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3 |
| title_fullStr | <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3 |
| title_full_unstemmed | <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3 |
| title_short | <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Display Differential Proteomic Responses to the Silver(I) Compound, SBC3 |
| title_sort | i pseudomonas aeruginosa i and i staphylococcus aureus i display differential proteomic responses to the silver i compound sbc3 |
| topic | antimicrobial proteome silver <i>Pseudomonas</i> <i>Staphylococcus</i> |
| url | https://www.mdpi.com/2079-6382/12/2/348 |
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