Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro

Introduction: Candidiasis, as an opportunistic infection, is caused by the Candida species. Although Candida albicans is classified in the body as an endogenic flora, it plays an important role in creating Candida related diseases. Candida vulvovaginitis in pregnant women, diabetes mellitus patients...

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Main Authors: F Katiraee, Z Farahnejad, M Riazipoor, MH Yadegari, H Zarrinfar
Format: Article
Language:fas
Published: Shahid Sadoughi University of Medical Sciences 2006-10-01
Series:Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd
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Online Access:http://jssu.ssu.ac.ir/browse.php?a_id=635&slc_lang=en&sid=1&ftxt=1
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author F Katiraee
Z Farahnejad
M Riazipoor
MH Yadegari
H Zarrinfar
author_facet F Katiraee
Z Farahnejad
M Riazipoor
MH Yadegari
H Zarrinfar
author_sort F Katiraee
collection DOAJ
description Introduction: Candidiasis, as an opportunistic infection, is caused by the Candida species. Although Candida albicans is classified in the body as an endogenic flora, it plays an important role in creating Candida related diseases. Candida vulvovaginitis in pregnant women, diabetes mellitus patients and those using multiple antibiotics and contraceptive drugs demonstrates the high resistance of the organism against conventional medication. On the other hand, recurrent vaginitis disintegrates the long-term process of treatment in majority of the patients. The present research was done with the aim of determining the optimum conditions for susceptibility testing before retreatment of patients. Methods: 10 isolates of Candida albicans obtained from 31 suspected patients suffering from recurrent Candida vaginitis were incubated with ketoconazole at two pH of 7.2 and 5.5 and two temperatures of 35ºC and 27ºC. The Microdilution broth test technique was used. The RPMI 1640 medium within the 96 well microplates with range of 12 tests was used to determine the MIC50 , MIC90 and MFC of the drug. Results: The obtained MIC50, MIC90 and MFC for ketoconazole at these conditions (T=35ºC and pH=7.2) were 0.25 to 1 µg/ml, 1 to 4 µg/ml and 64 to ≥ 512 µg/ml respectively, while these values at 27ºC, pH 5.5 were 1 to 8 µg/ml, 8 to 64 µg/ml and 512 to ≥ 512 µg/ml, at 35ºC and pH 5.5 the values were 1 to 8 µg/ml, 4 to 32 µg/ml, 256 to ≥ 512 µg/ml, while at 27ºC and pH 7.2 the values were 1 to 2 µg/ml, 8 to 32 µg/ml, 128 to ≥ 512 µg/ml, respectively. Conclusion: The obtained results confirmed that conditions with temperature of 35ºC and pH 7.2 resulted in better treatment outcomes than other conditions.
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spelling doaj.art-8c462e2aacd8438eb6d22537e794d2b22022-12-22T01:01:56ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332006-10-011435055Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole InvitroF KatiraeeZ FarahnejadM RiazipoorMH YadegariH ZarrinfarIntroduction: Candidiasis, as an opportunistic infection, is caused by the Candida species. Although Candida albicans is classified in the body as an endogenic flora, it plays an important role in creating Candida related diseases. Candida vulvovaginitis in pregnant women, diabetes mellitus patients and those using multiple antibiotics and contraceptive drugs demonstrates the high resistance of the organism against conventional medication. On the other hand, recurrent vaginitis disintegrates the long-term process of treatment in majority of the patients. The present research was done with the aim of determining the optimum conditions for susceptibility testing before retreatment of patients. Methods: 10 isolates of Candida albicans obtained from 31 suspected patients suffering from recurrent Candida vaginitis were incubated with ketoconazole at two pH of 7.2 and 5.5 and two temperatures of 35ºC and 27ºC. The Microdilution broth test technique was used. The RPMI 1640 medium within the 96 well microplates with range of 12 tests was used to determine the MIC50 , MIC90 and MFC of the drug. Results: The obtained MIC50, MIC90 and MFC for ketoconazole at these conditions (T=35ºC and pH=7.2) were 0.25 to 1 µg/ml, 1 to 4 µg/ml and 64 to ≥ 512 µg/ml respectively, while these values at 27ºC, pH 5.5 were 1 to 8 µg/ml, 8 to 64 µg/ml and 512 to ≥ 512 µg/ml, at 35ºC and pH 5.5 the values were 1 to 8 µg/ml, 4 to 32 µg/ml, 256 to ≥ 512 µg/ml, while at 27ºC and pH 7.2 the values were 1 to 2 µg/ml, 8 to 32 µg/ml, 128 to ≥ 512 µg/ml, respectively. Conclusion: The obtained results confirmed that conditions with temperature of 35ºC and pH 7.2 resulted in better treatment outcomes than other conditions.http://jssu.ssu.ac.ir/browse.php?a_id=635&slc_lang=en&sid=1&ftxt=1Candida AlbicansKetoconazoleVulvovaginitisTemperaturepH
spellingShingle F Katiraee
Z Farahnejad
M Riazipoor
MH Yadegari
H Zarrinfar
Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd
Candida Albicans
Ketoconazole
Vulvovaginitis
Temperature
pH
title Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro
title_full Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro
title_fullStr Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro
title_full_unstemmed Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro
title_short Evaluation of the Effects of Incubation Temperature and Ph On the Susceptibility of Candida Albicans Isolates to Ketoconazole Invitro
title_sort evaluation of the effects of incubation temperature and ph on the susceptibility of candida albicans isolates to ketoconazole invitro
topic Candida Albicans
Ketoconazole
Vulvovaginitis
Temperature
pH
url http://jssu.ssu.ac.ir/browse.php?a_id=635&slc_lang=en&sid=1&ftxt=1
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