Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes
Introduction: Recently we established that pro-inflammatory actions of angiotensin (Ang) II in astrocytes involved Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), and interleukin-6 (IL-6). Materials and methods: In our current study, we determined in brainstem and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2015-12-01
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| Series: | Journal of the Renin-Angiotensin-Aldosterone System |
| Online Access: | https://doi.org/10.1177/1470320314534509 |
| _version_ | 1797283737782714368 |
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| author | Umadevi Kandalam Nancy Sarmiento Dhanush Haspula Michelle A Clark |
| author_facet | Umadevi Kandalam Nancy Sarmiento Dhanush Haspula Michelle A Clark |
| author_sort | Umadevi Kandalam |
| collection | DOAJ |
| description | Introduction: Recently we established that pro-inflammatory actions of angiotensin (Ang) II in astrocytes involved Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), and interleukin-6 (IL-6). Materials and methods: In our current study, we determined in brainstem and cerebellum whether Ang III also activates STAT3 leading to IL-6 mRNA expression and astrocyte proliferation. Results: Ang III induced STAT3 phosphorylation in a concentration- and time-dependent manner. Significant STAT3 phosphorylation was rapid and was maximal within 10 min, and with 100 nM Ang III. The Ang AT 1 receptor was shown to mediate this action of Ang III. Ang III also significantly induced IL-6 mRNA expression within an hour, and maximal Ang III-mediated IL-6 mRNA expression occurred in the presence of 100 nM Ang III. Ang III-mediated IL-6 mRNA expression occurred by the interaction of the peptide with the Ang AT 1 receptor and was mediated by STAT3. In addition, STAT3 was shown to mediate Ang III astrocyte proliferation. Conclusions: These findings suggest that Ang III, similar to Ang II, has pro-inflammatory effects since it induces STAT3 leading to an induction of IL-6 mRNA expression, outcomes that lend relevance to the physiological importance of central Ang III. |
| first_indexed | 2024-03-07T17:35:13Z |
| format | Article |
| id | doaj.art-8c48ae7082be446c98bce71f865845b8 |
| institution | Directory Open Access Journal |
| issn | 1470-3203 1752-8976 |
| language | English |
| last_indexed | 2024-03-07T17:35:13Z |
| publishDate | 2015-12-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Journal of the Renin-Angiotensin-Aldosterone System |
| spelling | doaj.art-8c48ae7082be446c98bce71f865845b82024-03-02T16:57:47ZengSAGE PublicationsJournal of the Renin-Angiotensin-Aldosterone System1470-32031752-89762015-12-011610.1177/1470320314534509Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytesUmadevi Kandalam0Nancy Sarmiento1Dhanush Haspula2Michelle A Clark3Department of Pediatric Dentistry, Nova Southeastern University, USAFarquhar College of Arts and Sciences, Nova Southeastern University, USADepartment of Pharmaceutical Sciences, Nova Southeastern University, USADepartment of Pharmaceutical Sciences, Nova Southeastern University, USAIntroduction: Recently we established that pro-inflammatory actions of angiotensin (Ang) II in astrocytes involved Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), and interleukin-6 (IL-6). Materials and methods: In our current study, we determined in brainstem and cerebellum whether Ang III also activates STAT3 leading to IL-6 mRNA expression and astrocyte proliferation. Results: Ang III induced STAT3 phosphorylation in a concentration- and time-dependent manner. Significant STAT3 phosphorylation was rapid and was maximal within 10 min, and with 100 nM Ang III. The Ang AT 1 receptor was shown to mediate this action of Ang III. Ang III also significantly induced IL-6 mRNA expression within an hour, and maximal Ang III-mediated IL-6 mRNA expression occurred in the presence of 100 nM Ang III. Ang III-mediated IL-6 mRNA expression occurred by the interaction of the peptide with the Ang AT 1 receptor and was mediated by STAT3. In addition, STAT3 was shown to mediate Ang III astrocyte proliferation. Conclusions: These findings suggest that Ang III, similar to Ang II, has pro-inflammatory effects since it induces STAT3 leading to an induction of IL-6 mRNA expression, outcomes that lend relevance to the physiological importance of central Ang III.https://doi.org/10.1177/1470320314534509 |
| spellingShingle | Umadevi Kandalam Nancy Sarmiento Dhanush Haspula Michelle A Clark Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes Journal of the Renin-Angiotensin-Aldosterone System |
| title | Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes |
| title_full | Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes |
| title_fullStr | Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes |
| title_full_unstemmed | Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes |
| title_short | Angiotensin III induces signal transducer and activator of transcription 3 and interleukin-6 mRNA levels in cultured rat astrocytes |
| title_sort | angiotensin iii induces signal transducer and activator of transcription 3 and interleukin 6 mrna levels in cultured rat astrocytes |
| url | https://doi.org/10.1177/1470320314534509 |
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