Antioxidant Activity of Daemonorops draco Resin
Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study w...
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| Format: | Article |
| Language: | English |
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Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2019-09-01
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| Series: | Jurnal Kimia Sains dan Aplikasi |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/23016 |
| _version_ | 1798021083427766272 |
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| author | Sri Purwanti Wulan Tri Wahyuni Irmanida Batubara |
| author_facet | Sri Purwanti Wulan Tri Wahyuni Irmanida Batubara |
| author_sort | Sri Purwanti |
| collection | DOAJ |
| description | Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene. |
| first_indexed | 2024-04-11T17:08:15Z |
| format | Article |
| id | doaj.art-8c4b4a7c42274fe4b222093ad82089d2 |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-04-11T17:08:15Z |
| publishDate | 2019-09-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-8c4b4a7c42274fe4b222093ad82089d22022-12-22T04:12:59ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142019-09-0122517918310.14710/jksa.22.5.179-18315520Antioxidant Activity of Daemonorops draco ResinSri Purwanti0Wulan Tri Wahyuni1Irmanida Batubara2https://orcid.org/0000-0001-8201-7807Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, IPB University, IndonesiaJernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.https://ejournal.undip.ac.id/index.php/ksa/article/view/23016antioxidantchromatographydaemonorops dracofractionationresin |
| spellingShingle | Sri Purwanti Wulan Tri Wahyuni Irmanida Batubara Antioxidant Activity of Daemonorops draco Resin Jurnal Kimia Sains dan Aplikasi antioxidant chromatography daemonorops draco fractionation resin |
| title | Antioxidant Activity of Daemonorops draco Resin |
| title_full | Antioxidant Activity of Daemonorops draco Resin |
| title_fullStr | Antioxidant Activity of Daemonorops draco Resin |
| title_full_unstemmed | Antioxidant Activity of Daemonorops draco Resin |
| title_short | Antioxidant Activity of Daemonorops draco Resin |
| title_sort | antioxidant activity of daemonorops draco resin |
| topic | antioxidant chromatography daemonorops draco fractionation resin |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/23016 |
| work_keys_str_mv | AT sripurwanti antioxidantactivityofdaemonoropsdracoresin AT wulantriwahyuni antioxidantactivityofdaemonoropsdracoresin AT irmanidabatubara antioxidantactivityofdaemonoropsdracoresin |