In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography
Recent developments in melt electrowriting (MEW), a high‐resolution additive manufacturing technology, have led to increases in scaffold complexity. However, MEW scaffolds are currently characterized ex situ, which causes time–consuming iterations of characterization and fabrication that limit scaff...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2022-07-01
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| Series: | Advanced Photonics Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/adpr.202100274 |
| _version_ | 1828775622489407488 |
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| author | Evelyn Collier Brooke Maitland Rowan W. Sanderson Behzad Shiroud Heidari Christopher Lamb Matt S. Hepburn Paul D. Dalton Qi Fang Elena M. De-Juan-Pardo Brendan F. Kennedy |
| author_facet | Evelyn Collier Brooke Maitland Rowan W. Sanderson Behzad Shiroud Heidari Christopher Lamb Matt S. Hepburn Paul D. Dalton Qi Fang Elena M. De-Juan-Pardo Brendan F. Kennedy |
| author_sort | Evelyn Collier |
| collection | DOAJ |
| description | Recent developments in melt electrowriting (MEW), a high‐resolution additive manufacturing technology, have led to increases in scaffold complexity. However, MEW scaffolds are currently characterized ex situ, which causes time–consuming iterations of characterization and fabrication that limit scaffold throughput and more widespread use of the technology. For the first time, an in situ method to characterize the 3D microstructure of MEW scaffolds using optical coherence tomography (OCT) is described. Calculations of microstructural features are performed on OCT data using a custom algorithm, demonstrating close correspondence with scanning electron microscopy (SEM). For example, OCT calculations of fiber diameter and scaffold thickness are within an average of 0.31 and 1.79 μm, respectively, of corresponding SEM–derived calculations. Additionally, the 3D capabilities of OCT enable the nondestructive characterization of scaffolds with depth–varying microstructures, overcoming some main limitations of SEM. Finally, in situ characterization is achieved by integrating the OCT scanner within an MEW printer, enabling the scaffold microstructure to be evaluated and optimized during manufacture. This new capability represents an important step toward achieving an efficient fabrication–characterization cycle with the guaranteed scaffold quality and reproducibility required to validate the manufacturing process. |
| first_indexed | 2024-12-11T15:43:45Z |
| format | Article |
| id | doaj.art-8c4c5128ff4d412f9f424342a103b703 |
| institution | Directory Open Access Journal |
| issn | 2699-9293 |
| language | English |
| last_indexed | 2024-12-11T15:43:45Z |
| publishDate | 2022-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Photonics Research |
| spelling | doaj.art-8c4c5128ff4d412f9f424342a103b7032022-12-22T00:59:46ZengWiley-VCHAdvanced Photonics Research2699-92932022-07-0137n/an/a10.1002/adpr.202100274In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence TomographyEvelyn Collier0Brooke Maitland1Rowan W. Sanderson2Behzad Shiroud Heidari3Christopher Lamb4Matt S. Hepburn5Paul D. Dalton6Qi Fang7Elena M. De-Juan-Pardo8Brendan F. Kennedy9BRITElab Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaBRITElab Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaBRITElab Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaT3mPLATE Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaT3mPLATE Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaBRITElab Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaPhil and Penny Knight Campus for Accelerating Scientific Impact University of Oregon Eugene OR 97403 USABRITElab Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaT3mPLATE Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaBRITElab Harry Perkins Institute of Medical Research QEII Medical Centre Nedlands and Centre for Medical Research The University of Western Australia Perth WA 6009 AustraliaRecent developments in melt electrowriting (MEW), a high‐resolution additive manufacturing technology, have led to increases in scaffold complexity. However, MEW scaffolds are currently characterized ex situ, which causes time–consuming iterations of characterization and fabrication that limit scaffold throughput and more widespread use of the technology. For the first time, an in situ method to characterize the 3D microstructure of MEW scaffolds using optical coherence tomography (OCT) is described. Calculations of microstructural features are performed on OCT data using a custom algorithm, demonstrating close correspondence with scanning electron microscopy (SEM). For example, OCT calculations of fiber diameter and scaffold thickness are within an average of 0.31 and 1.79 μm, respectively, of corresponding SEM–derived calculations. Additionally, the 3D capabilities of OCT enable the nondestructive characterization of scaffolds with depth–varying microstructures, overcoming some main limitations of SEM. Finally, in situ characterization is achieved by integrating the OCT scanner within an MEW printer, enabling the scaffold microstructure to be evaluated and optimized during manufacture. This new capability represents an important step toward achieving an efficient fabrication–characterization cycle with the guaranteed scaffold quality and reproducibility required to validate the manufacturing process.https://doi.org/10.1002/adpr.202100274electrohydrodynamicsimagingin situ characterizationmelt electrospinningmelt electrowritingoptical coherence tomography |
| spellingShingle | Evelyn Collier Brooke Maitland Rowan W. Sanderson Behzad Shiroud Heidari Christopher Lamb Matt S. Hepburn Paul D. Dalton Qi Fang Elena M. De-Juan-Pardo Brendan F. Kennedy In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography Advanced Photonics Research electrohydrodynamics imaging in situ characterization melt electrospinning melt electrowriting optical coherence tomography |
| title | In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography |
| title_full | In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography |
| title_fullStr | In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography |
| title_full_unstemmed | In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography |
| title_short | In Situ Characterization of Melt–Electrowritten Scaffolds in 3D Using Optical Coherence Tomography |
| title_sort | in situ characterization of melt electrowritten scaffolds in 3d using optical coherence tomography |
| topic | electrohydrodynamics imaging in situ characterization melt electrospinning melt electrowriting optical coherence tomography |
| url | https://doi.org/10.1002/adpr.202100274 |
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