The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2006-11-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700016 |
| _version_ | 1797757125732073472 |
|---|---|
| author | Claudio R Madruga Flábio R Araújo Gustavo Cavalcante-Goes Charles Martins Ingá B Pfeifer Laura R Ribeiro Raul H Kessler Cleber O Soares Midori Miguita Elaine PS Melo Robson FC Almeida Manoel MSC Lima Jr. |
| author_facet | Claudio R Madruga Flábio R Araújo Gustavo Cavalcante-Goes Charles Martins Ingá B Pfeifer Laura R Ribeiro Raul H Kessler Cleber O Soares Midori Miguita Elaine PS Melo Robson FC Almeida Manoel MSC Lima Jr. |
| author_sort | Claudio R Madruga |
| collection | DOAJ |
| description | There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction. |
| first_indexed | 2024-03-12T18:11:29Z |
| format | Article |
| id | doaj.art-8c4d7ffb7bd244efbd60a6679fcd061a |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:11:29Z |
| publishDate | 2006-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-8c4d7ffb7bd244efbd60a6679fcd061a2023-08-02T09:15:32ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602006-11-01101780180710.1590/S0074-02762006000700016The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveysClaudio R MadrugaFlábio R AraújoGustavo Cavalcante-GoesCharles MartinsIngá B PfeiferLaura R RibeiroRaul H KesslerCleber O SoaresMidori MiguitaElaine PS MeloRobson FC AlmeidaManoel MSC Lima Jr.There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700016Trypanosoma vivaxenzymelinked immunosorbent assayepidemiological surveycattlePantanalBrazil |
| spellingShingle | Claudio R Madruga Flábio R Araújo Gustavo Cavalcante-Goes Charles Martins Ingá B Pfeifer Laura R Ribeiro Raul H Kessler Cleber O Soares Midori Miguita Elaine PS Melo Robson FC Almeida Manoel MSC Lima Jr. The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys Memorias do Instituto Oswaldo Cruz Trypanosoma vivax enzyme linked immunosorbent assay epidemiological survey cattle Pantanal Brazil |
| title | The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys |
| title_full | The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys |
| title_fullStr | The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys |
| title_full_unstemmed | The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys |
| title_short | The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys |
| title_sort | development of an enzyme linked immunosorbent assay for trypanosoma vivax antibodies and its use in epidemiological surveys |
| topic | Trypanosoma vivax enzyme linked immunosorbent assay epidemiological survey cattle Pantanal Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700016 |
| work_keys_str_mv | AT claudiormadruga thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT flabioraraujo thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT gustavocavalcantegoes thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT charlesmartins thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT ingabpfeifer thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT laurarribeiro thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT raulhkessler thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT cleberosoares thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT midorimiguita thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT elainepsmelo thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT robsonfcalmeida thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT manoelmsclimajr thedevelopmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT claudiormadruga developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT flabioraraujo developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT gustavocavalcantegoes developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT charlesmartins developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT ingabpfeifer developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT laurarribeiro developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT raulhkessler developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT cleberosoares developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT midorimiguita developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT elainepsmelo developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT robsonfcalmeida developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys AT manoelmsclimajr developmentofanenzymelinkedimmunosorbentassayfortrypanosomavivaxantibodiesanditsuseinepidemiologicalsurveys |