Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats
Cardiovascular disease represents a vital global disease burden. This study aims to assess the possible cardioprotective effect of thymol against adrenaline-induced myocardial injury (MI) in rats. Furthermore the effect of thymol on cardiac function biomarkers, electrocardiogram (ECG) alterations, o...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020312755 |
| _version_ | 1818527901204611072 |
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| author | Salma A. El-Marasy Sally A. El Awdan Azza Hassan Heba M.I. Abdallah |
| author_facet | Salma A. El-Marasy Sally A. El Awdan Azza Hassan Heba M.I. Abdallah |
| author_sort | Salma A. El-Marasy |
| collection | DOAJ |
| description | Cardiovascular disease represents a vital global disease burden. This study aims to assess the possible cardioprotective effect of thymol against adrenaline-induced myocardial injury (MI) in rats. Furthermore the effect of thymol on cardiac function biomarkers, electrocardiogram (ECG) alterations, oxidative stress, inflammation, apoptosis and histopathological changes was assessed. MI was induced by adrenaline (2 mg/kg, s.c.) injected as a single dose for 2 consecutive days (24 h apart). Normal and control groups received the vehicle for 21 consecutive days. The other 3 groups were orally administered thymol (15, 30, 60 mg/kg) for 21 consecutive days and on day 22, adrenaline was injected as a single dose for 2 consecutive days. Then ECG examination, biochemical, histopathological, immunohistochemical analyses were carried out. Thymol reversed adrenaline-induced reduction of heart rate, prolongation of RR interval and elevation of ST interval. Thymol pretreatment significantly reduced serum aspartate dehydrogenase (AST), lactate dehydrogenase (LDH), and creatine kinase (CK) levels in MI rats. Oral pretreatment with thymol increased reduced glutathione (GSH), reduced malondialdehyde (MDA), nuclear factor-kappa B (NF-κB), and interleukin-1β (IL-1β) cardiac contents in MI rats. Additionally, thymol administration significantly decreased protein expression of caspase-3, increased Bcl-2 protein expression in cardiac tissue and ameliorated histopathological changes. This study reveals that thymol exerted cardioprotective effect against adrenaline-induced MI in rats evidenced by improving cardiac function, attenuating ECG and histopathological changes which may be partly mediated through its anti-oxidant, anti-inflammatory and anti-apoptotic effect. |
| first_indexed | 2024-12-11T06:42:29Z |
| format | Article |
| id | doaj.art-8c51d417fd454e8c9e8b57053c7b59e6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-11T06:42:29Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8c51d417fd454e8c9e8b57053c7b59e62022-12-22T01:17:11ZengElsevierHeliyon2405-84402020-07-0167e04431Cardioprotective effect of thymol against adrenaline-induced myocardial injury in ratsSalma A. El-Marasy0Sally A. El Awdan1Azza Hassan2Heba M.I. Abdallah3Department of Pharmacology, National Research Centre, Giza, Egypt; Corresponding author.Department of Pharmacology, National Research Centre, Giza, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Pharmacology, National Research Centre, Giza, EgyptCardiovascular disease represents a vital global disease burden. This study aims to assess the possible cardioprotective effect of thymol against adrenaline-induced myocardial injury (MI) in rats. Furthermore the effect of thymol on cardiac function biomarkers, electrocardiogram (ECG) alterations, oxidative stress, inflammation, apoptosis and histopathological changes was assessed. MI was induced by adrenaline (2 mg/kg, s.c.) injected as a single dose for 2 consecutive days (24 h apart). Normal and control groups received the vehicle for 21 consecutive days. The other 3 groups were orally administered thymol (15, 30, 60 mg/kg) for 21 consecutive days and on day 22, adrenaline was injected as a single dose for 2 consecutive days. Then ECG examination, biochemical, histopathological, immunohistochemical analyses were carried out. Thymol reversed adrenaline-induced reduction of heart rate, prolongation of RR interval and elevation of ST interval. Thymol pretreatment significantly reduced serum aspartate dehydrogenase (AST), lactate dehydrogenase (LDH), and creatine kinase (CK) levels in MI rats. Oral pretreatment with thymol increased reduced glutathione (GSH), reduced malondialdehyde (MDA), nuclear factor-kappa B (NF-κB), and interleukin-1β (IL-1β) cardiac contents in MI rats. Additionally, thymol administration significantly decreased protein expression of caspase-3, increased Bcl-2 protein expression in cardiac tissue and ameliorated histopathological changes. This study reveals that thymol exerted cardioprotective effect against adrenaline-induced MI in rats evidenced by improving cardiac function, attenuating ECG and histopathological changes which may be partly mediated through its anti-oxidant, anti-inflammatory and anti-apoptotic effect.http://www.sciencedirect.com/science/article/pii/S2405844020312755ThymolAdrenalineMyocardial injuryECG examinationOxidative stressInflammation |
| spellingShingle | Salma A. El-Marasy Sally A. El Awdan Azza Hassan Heba M.I. Abdallah Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats Heliyon Thymol Adrenaline Myocardial injury ECG examination Oxidative stress Inflammation |
| title | Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats |
| title_full | Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats |
| title_fullStr | Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats |
| title_full_unstemmed | Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats |
| title_short | Cardioprotective effect of thymol against adrenaline-induced myocardial injury in rats |
| title_sort | cardioprotective effect of thymol against adrenaline induced myocardial injury in rats |
| topic | Thymol Adrenaline Myocardial injury ECG examination Oxidative stress Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020312755 |
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