MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum
The mechanism of learning and memory impairment induced by the combination of fluorine and aluminum (FA) is not fully understood. The results of our previous research demonstrated that miR-29b-3p is a differentially expressed miRNA in the hippocampi of rat offspring exposed to FA; this miRNA is rela...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321007703 |
| _version_ | 1818730254524481536 |
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| author | Zhongbi Peng Xuemei Yang Hua Zhang Mingyue Yin Yu Luo Chun Xie |
| author_facet | Zhongbi Peng Xuemei Yang Hua Zhang Mingyue Yin Yu Luo Chun Xie |
| author_sort | Zhongbi Peng |
| collection | DOAJ |
| description | The mechanism of learning and memory impairment induced by the combination of fluorine and aluminum (FA) is not fully understood. The results of our previous research demonstrated that miR-29b-3p is a differentially expressed miRNA in the hippocampi of rat offspring exposed to FA; this miRNA is related to learning and memory and apoptosis. Based on these findings, in vitro studies were designed to assess the role of miR-29b-3p in neuronal apoptosis caused by the coexistence of FA. In the present study, the viability of mouse neuroblastoma–rat glioma hybrid cell (NG108–15 cell) was analyzed using Cell Counting Kit-8 (CCK-8). Apoptosis was detected by a Novocyte Flow Cytometer. Relative mRNA and protein expression levels were evaluated by real-time fluorescence quantitative PCR (qRT-PCR) and Western blotting (WB), respectively. The results showed that FA aggravated NG108–15 cell apoptosis by inhibiting dual-specificity phosphatase-2 (Dusp2) via increased miR-29b-3p. Accordingly, a dual-luciferase reporter assay showed that miR-29b-3p modulated Dusp2 protein levels by targeting its 3′-untranslated region. These findings show, for the first time, that miR-29b-3p is involved in neuronal apoptosis triggered by FA by targeting Dusp2. |
| first_indexed | 2024-12-17T22:58:52Z |
| format | Article |
| id | doaj.art-8c51dbae3e77439bb6c16381f69f4d14 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-17T22:58:52Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-8c51dbae3e77439bb6c16381f69f4d142022-12-21T21:29:28ZengElsevierEcotoxicology and Environmental Safety0147-65132021-11-01224112658MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminumZhongbi Peng0Xuemei Yang1Hua Zhang2Mingyue Yin3Yu Luo4Chun Xie5School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaCorrespondence to: Guizhou Medical University, Guian New Area University City, Guizhou 550025, China.; School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaThe mechanism of learning and memory impairment induced by the combination of fluorine and aluminum (FA) is not fully understood. The results of our previous research demonstrated that miR-29b-3p is a differentially expressed miRNA in the hippocampi of rat offspring exposed to FA; this miRNA is related to learning and memory and apoptosis. Based on these findings, in vitro studies were designed to assess the role of miR-29b-3p in neuronal apoptosis caused by the coexistence of FA. In the present study, the viability of mouse neuroblastoma–rat glioma hybrid cell (NG108–15 cell) was analyzed using Cell Counting Kit-8 (CCK-8). Apoptosis was detected by a Novocyte Flow Cytometer. Relative mRNA and protein expression levels were evaluated by real-time fluorescence quantitative PCR (qRT-PCR) and Western blotting (WB), respectively. The results showed that FA aggravated NG108–15 cell apoptosis by inhibiting dual-specificity phosphatase-2 (Dusp2) via increased miR-29b-3p. Accordingly, a dual-luciferase reporter assay showed that miR-29b-3p modulated Dusp2 protein levels by targeting its 3′-untranslated region. These findings show, for the first time, that miR-29b-3p is involved in neuronal apoptosis triggered by FA by targeting Dusp2.http://www.sciencedirect.com/science/article/pii/S0147651321007703FluorineAluminumDusp2ApoptosisMiR-29b-3p |
| spellingShingle | Zhongbi Peng Xuemei Yang Hua Zhang Mingyue Yin Yu Luo Chun Xie MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum Ecotoxicology and Environmental Safety Fluorine Aluminum Dusp2 Apoptosis MiR-29b-3p |
| title | MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum |
| title_full | MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum |
| title_fullStr | MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum |
| title_full_unstemmed | MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum |
| title_short | MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum |
| title_sort | mir 29b 3p aggravates ng108 15 cell apoptosis triggered by fluorine combined with aluminum |
| topic | Fluorine Aluminum Dusp2 Apoptosis MiR-29b-3p |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321007703 |
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