Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species
Many recent articles feature research on the <i>Fusarium tricinctum</i> species complex (FTSC), and their authors present different ideas on how the isolates of this species complex can be identified at the species level. In previous studies, our aim was to investigate the phylogeny of F...
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MDPI AG
2022-07-01
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| author | Tapani Yli-Mattila Asmaa Abbas Olga Gavrilova Tatiana Gagkaeva |
| author_facet | Tapani Yli-Mattila Asmaa Abbas Olga Gavrilova Tatiana Gagkaeva |
| author_sort | Tapani Yli-Mattila |
| collection | DOAJ |
| description | Many recent articles feature research on the <i>Fusarium tricinctum</i> species complex (FTSC), and their authors present different ideas on how the isolates of this species complex can be identified at the species level. In previous studies, our aim was to investigate the phylogeny of FTSC strains, which researchers have morphologically identified as <i>Fusarium avenaceum</i>, <i>Fusarium arthrosporioides</i>, and <i>Fusarium anguioides.</i> In the current study, our phylogenetic maximum parsimony and likelihood analyses of the DNA sequences of the translation elongation factor 1-alpha (<i>TEF1</i>) and combined sequences of <i>TEF1</i> and beta-tubulin (<i>TUB2</i>) supported the existence of at least four main groups among these strains. Main Group I mainly contains <i>F. avenaceum</i> strains, while Main Group II contains two subgroups, one of which primarily includes <i>F. arthrosporioides</i> strains, and the other mainly includes European <i>F. anguioides</i> strains. Main Group III contains strains from different plants that originated from Asia, including two <i>F. anguioides</i> strains. <i>F. avenaceum</i> strains, which are mostly isolated from different trees, form Main Group IV. A fifth group (Main Group V) was only supported by <i>TEF1</i> sequences. The main groups previously found by us based on <i>TUB2</i> sequences could be connected to the new species of the FTSC, which were identified based on <i>TEF1</i> sequences. In addition, we found strains that significantly differ from Main Groups I-V, and we grouped some of them as single, intermediate, or sister groups. All of the main groups of the present work, and some single and intermediate strains, may represent different species of the FTSC, while the two subgroups of Main Group II constitute intraspecific variation. Regardless of whether they belonged to the main groups, all the analysed strains were able to form different enniatins and 2-amino-14,16-dimethyloctadecan-3-ol, but did not produce beauvericin. |
| first_indexed | 2024-03-09T12:00:48Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T12:00:48Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-8c52866e345443f9881b183fecced5962023-11-30T23:04:33ZengMDPI AGDiversity1424-28182022-07-0114757410.3390/d14070574Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related SpeciesTapani Yli-Mattila0Asmaa Abbas1Olga Gavrilova2Tatiana Gagkaeva3Laboratory of Molecular Plant Biology, Department of Life Technologies, University of Turku, 20014 Turku, FinlandLaboratory of Molecular Plant Biology, Department of Life Technologies, University of Turku, 20014 Turku, FinlandLaboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), 196608 St. Petersburg, RussiaLaboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), 196608 St. Petersburg, RussiaMany recent articles feature research on the <i>Fusarium tricinctum</i> species complex (FTSC), and their authors present different ideas on how the isolates of this species complex can be identified at the species level. In previous studies, our aim was to investigate the phylogeny of FTSC strains, which researchers have morphologically identified as <i>Fusarium avenaceum</i>, <i>Fusarium arthrosporioides</i>, and <i>Fusarium anguioides.</i> In the current study, our phylogenetic maximum parsimony and likelihood analyses of the DNA sequences of the translation elongation factor 1-alpha (<i>TEF1</i>) and combined sequences of <i>TEF1</i> and beta-tubulin (<i>TUB2</i>) supported the existence of at least four main groups among these strains. Main Group I mainly contains <i>F. avenaceum</i> strains, while Main Group II contains two subgroups, one of which primarily includes <i>F. arthrosporioides</i> strains, and the other mainly includes European <i>F. anguioides</i> strains. Main Group III contains strains from different plants that originated from Asia, including two <i>F. anguioides</i> strains. <i>F. avenaceum</i> strains, which are mostly isolated from different trees, form Main Group IV. A fifth group (Main Group V) was only supported by <i>TEF1</i> sequences. The main groups previously found by us based on <i>TUB2</i> sequences could be connected to the new species of the FTSC, which were identified based on <i>TEF1</i> sequences. In addition, we found strains that significantly differ from Main Groups I-V, and we grouped some of them as single, intermediate, or sister groups. All of the main groups of the present work, and some single and intermediate strains, may represent different species of the FTSC, while the two subgroups of Main Group II constitute intraspecific variation. Regardless of whether they belonged to the main groups, all the analysed strains were able to form different enniatins and 2-amino-14,16-dimethyloctadecan-3-ol, but did not produce beauvericin.https://www.mdpi.com/1424-2818/14/7/574<i>Fusarium anguioides</i><i>F. arthrosporioides</i><i>F. avenaceum</i><i>Fusarium tricinctum</i> species complexbeta-tubulinelongation factor 1-alpha |
| spellingShingle | Tapani Yli-Mattila Asmaa Abbas Olga Gavrilova Tatiana Gagkaeva Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species Diversity <i>Fusarium anguioides</i> <i>F. arthrosporioides</i> <i>F. avenaceum</i> <i>Fusarium tricinctum</i> species complex beta-tubulin elongation factor 1-alpha |
| title | Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species |
| title_full | Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species |
| title_fullStr | Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species |
| title_full_unstemmed | Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species |
| title_short | Molecular Variation and Phylogeny within <i>Fusarium avenaceum</i> and Related Species |
| title_sort | molecular variation and phylogeny within i fusarium avenaceum i and related species |
| topic | <i>Fusarium anguioides</i> <i>F. arthrosporioides</i> <i>F. avenaceum</i> <i>Fusarium tricinctum</i> species complex beta-tubulin elongation factor 1-alpha |
| url | https://www.mdpi.com/1424-2818/14/7/574 |
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