Blue-violet light decreases VEGFa production in an in vitro model of AMD.
Blue light is an identified risk factor for age-related macular degeneration (AMD). The production of vascular endothelial growth factor (VEGF), leading to neovascularization, is a major complication of the wet form of this disease. We investigated how blue light affects VEGF expression and secretio...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0223839 |
| _version_ | 1819259590552846336 |
|---|---|
| author | Mélanie Marie Pauline Gondouin Delphine Pagan Coralie Barrau Thierry Villette José Sahel Serge Picaud |
| author_facet | Mélanie Marie Pauline Gondouin Delphine Pagan Coralie Barrau Thierry Villette José Sahel Serge Picaud |
| author_sort | Mélanie Marie |
| collection | DOAJ |
| description | Blue light is an identified risk factor for age-related macular degeneration (AMD). The production of vascular endothelial growth factor (VEGF), leading to neovascularization, is a major complication of the wet form of this disease. We investigated how blue light affects VEGF expression and secretion using A2E-loaded retinal pigment epithelium (RPE) cells, a cell model of AMD. Incubation of RPE cells with A2E resulted in a significant increase in VEGF mRNA and, intracellular and secreted VEGF protein levels, but not mRNA levels of VEGFR1 or VEGFR2. Blue light exposure of A2E-loaded RPE cells resulted in a decrease in VEGF mRNA and protein levels, but an increase in VEGFR1 levels. The toxicity of 440 nm light on A2E-loaded RPE cells was enhanced by VEGF supplementation. Our results suggest that age-related A2E accumulation may result in VEGF synthesis and release. This synthesis of VEGF, which enhances blue light toxicity for the RPE cells, is itself suppressed by blue light. Anti-VEGF therapy may therefore improve RPE survival in AMD. |
| first_indexed | 2024-12-23T19:12:26Z |
| format | Article |
| id | doaj.art-8c5588b18e5c4e78bc7d754fb6a2a7a1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:12:26Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8c5588b18e5c4e78bc7d754fb6a2a7a12022-12-21T17:34:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011410e022383910.1371/journal.pone.0223839Blue-violet light decreases VEGFa production in an in vitro model of AMD.Mélanie MariePauline GondouinDelphine PaganCoralie BarrauThierry VilletteJosé SahelSerge PicaudBlue light is an identified risk factor for age-related macular degeneration (AMD). The production of vascular endothelial growth factor (VEGF), leading to neovascularization, is a major complication of the wet form of this disease. We investigated how blue light affects VEGF expression and secretion using A2E-loaded retinal pigment epithelium (RPE) cells, a cell model of AMD. Incubation of RPE cells with A2E resulted in a significant increase in VEGF mRNA and, intracellular and secreted VEGF protein levels, but not mRNA levels of VEGFR1 or VEGFR2. Blue light exposure of A2E-loaded RPE cells resulted in a decrease in VEGF mRNA and protein levels, but an increase in VEGFR1 levels. The toxicity of 440 nm light on A2E-loaded RPE cells was enhanced by VEGF supplementation. Our results suggest that age-related A2E accumulation may result in VEGF synthesis and release. This synthesis of VEGF, which enhances blue light toxicity for the RPE cells, is itself suppressed by blue light. Anti-VEGF therapy may therefore improve RPE survival in AMD.https://doi.org/10.1371/journal.pone.0223839 |
| spellingShingle | Mélanie Marie Pauline Gondouin Delphine Pagan Coralie Barrau Thierry Villette José Sahel Serge Picaud Blue-violet light decreases VEGFa production in an in vitro model of AMD. PLoS ONE |
| title | Blue-violet light decreases VEGFa production in an in vitro model of AMD. |
| title_full | Blue-violet light decreases VEGFa production in an in vitro model of AMD. |
| title_fullStr | Blue-violet light decreases VEGFa production in an in vitro model of AMD. |
| title_full_unstemmed | Blue-violet light decreases VEGFa production in an in vitro model of AMD. |
| title_short | Blue-violet light decreases VEGFa production in an in vitro model of AMD. |
| title_sort | blue violet light decreases vegfa production in an in vitro model of amd |
| url | https://doi.org/10.1371/journal.pone.0223839 |
| work_keys_str_mv | AT melaniemarie bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd AT paulinegondouin bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd AT delphinepagan bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd AT coraliebarrau bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd AT thierryvillette bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd AT josesahel bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd AT sergepicaud bluevioletlightdecreasesvegfaproductioninaninvitromodelofamd |